重组Anti-p27 KIP 1 (phospho S10)抗体[EP233(2)Y] (ab62364)
![Western blot - Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] (ab62364)](https://www.abcam.cn/ps/products/62/ab62364/Images/ab62364-450825-anti-p27-kip-1-phospho-s10-antibody-ep233-2-y-westernblot-human-mouse-rat.jpg "Western blot - Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] (ab62364)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP233(2)Y] to p27 KIP 1 (phospho S10)
- Suitable for: Dot blot, WB, IP, IHC-P
- Reacts with: Mouse, Rat, Human, Monkey
Related conjugates and formulations
概述
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产品名称
Anti-p27 KIP 1 (phospho S10)抗体[EP233(2)Y]
参阅全部 p27 KIP 1 一抗 -
描述
兔单克隆抗体[EP233(2)Y] to p27 KIP 1 (phospho S10) -
宿主
Rabbit -
特异性
This antibody detects p27 KIP 1 phosphorylated at Serine 10.
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
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经测试应用
适用于: Dot blot, WB, IP, IHC-Pmore details
不适用于: Flow Cyt or ICC/IF -
种属反应性
与反应: Mouse, Rat, Human, Monkey -
免疫原
Synthetic peptide within Human p27 KIP 1 aa 1-100 (phospho S10) (Cysteine residue). The exact sequence is proprietary.
Database link: P46527 -
阳性对照
- IP: MCF7 cell lysate; IHC: Human colon cancer; WB: HeLa, NIH/3T3, and C6 whole cell lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP233(2)Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab62364于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Dot blot |
1/1000.
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| WB | (1) |
1/500 - 1/20000. Detects a band of approximately 27 kDa (predicted molecular weight: 22 kDa).
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| IP |
1/50.
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| IHC-P | (4) |
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
See IHC antigen retrieval protocols. For unpurified use at 1/200 - 1/1500 The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. |
| 说明 |
|---|
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Dot blot
1/1000. |
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WB
1/500 - 1/20000. Detects a band of approximately 27 kDa (predicted molecular weight: 22 kDa). |
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IP
1/50. |
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IHC-P
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/200 - 1/1500 The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. |
靶标
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功能
Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry. -
组织特异性
Expressed in all tissues tested. Highest levels in skeletal muscle, lowest in liver and kidney. -
疾病相关
Defects in CDKN1B are the cause of multiple endocrine neoplasia type 4 (MEN4) [MIM:610755]. Multiple endocrine neoplasia (MEN) syndromes are inherited cancer syndromes of the thyroid. MEN4 is a MEN-like syndrome with a phenotypic overlap of both MEN1 and MEN2. -
序列相似性
Belongs to the CDI family. -
结构域
A peptide sequence containing only AA 28-79 retains substantial Kip1 cyclin A/CDK2 inhibitory activity. -
翻译后修饰
Phosphorylated; phosphorylation occurs on serine, threonine and tyrosine residues. Phosphorylation on Ser-10 is the major site of phosphorylation in resting cells, takes place at the G(0)-G(1) phase and leads to protein stability. Phosphorylation on other sites is greatly enhanced by mitogens, growth factors, cMYC and in certain cancer cell lines. The phosphorylated form found in the cytoplasm is inactivate. Phosphorylation on Thr-198 is required for interaction with 14-3-3 proteins. Phosphorylation on Thr-187, by CDK2 leads to protein ubiquitination and proteasomal degradation. Tyrosine phosphorylation promotes this process. Phosphorylation by PKB/AKT1 can be suppressed by LY294002, an inhibitor of the catalytic subunit of PI3K. Phosphorylation on Tyr-88 and Tyr-89 has no effect on binding CDK2, but is required for binding CDK4. Dephosphorylated on tyrosine residues by G-CSF.
Ubiquitinated; in the cytoplasm by the KPC complex (composed of RNF123/KPC1 and UBAC1/KPC2) and, in the nucleus, by SCF(SKP2). The latter requires prior phosphorylation on Thr-187. Ubiquitinated; by a TRIM21-containing SCF(SKP2)-like complex; leads to its degradation.
Subject to degradation in the lysosome. Interaction with SNX6 promotes lysosomal degradation. -
细胞定位
Nucleus. Cytoplasm. Endosome. Nuclear and cytoplasmic in quiescent cells. AKT-or RSK-mediated phosphorylation on Thr-198, binds 14-3-3, translocates to the cytoplasm and promotes cell cycle progression. Mitogen-activated UHMK1 phosphorylation on Ser-10 also results in translocation to the cytoplasm and cell cycle progression. Phosphorylation on Ser-10 facilitates nuclear export. Translocates to the nucleus on phosphorylation of Tyr-88 and Tyr-89. Colocalizes at the endosome with SNX6 and this leads to lysosomal degradation. - Information by UniProt
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数据库链接
- Entrez Gene: 1027 Human
- Entrez Gene: 12576 Mouse
- Entrez Gene: 83571 Rat
- Omim: 600778 Human
- SwissProt: P46527 Human
- SwissProt: P46414 Mouse
- Unigene: 238990 Human
- Unigene: 2958 Mouse
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别名
- AA408329 antibody
- AI843786 antibody
- Cdki1b antibody
see all
图片
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Western blot - Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] (ab62364)All lanes : Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] (ab62364) at 1/1000 dilution (Purified)
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate, membrane treated with Alkaline Phosphatase for 1 hour
Lane 3 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
Lane 4 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate, membrane treated with Alkaline Phosphatase for 1 hour
Lane 5 : C6 (Rat glial tumor glial cell) whole cell lysate
Lane 6 : C6 (Rat glial tumor glial cell) whole cell lysate, membrane treated with Alkaline Phosphatase for 1 hour
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 22 kDa
Observed band size: 27 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] (ab62364)](https://www.abcam.cn/ps/products/62/ab62364/Images/ab62364-10-anti-p27-kip-1-phospho-s10-antibody-ep233-2-y-ihc-colon-cancer-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] (ab62364)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon cancer tissue sections labeling p27 KIP 1 with Purified ab62364 at 1:100 dilution (4.19 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain. -
![Immunoprecipitation - Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] (ab62364)](https://www.abcam.cn/ps/products/62/ab62364/Images/ab62364-9-anti-p27-kip-1-phospho-s10-antibody-ep233-2-y-immunoprecipitation-mcf7-human.jpg)
Immunoprecipitation - Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] (ab62364)Purified ab62364 at 1/50 dilution (2µg) immunoprecipitating p27 KIP 1 in MCF7 whole cell lysate.
Lane 1 (input): MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate 10µg
Lane 2 (+): ab62364 + MCF7 whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab62364 in MCF7 whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 27 kDa -
![Dot Blot - Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] (ab62364)](https://www.abcam.cn/ps/products/62/ab62364/Images/ab62364-269884-3.jpg)
Dot Blot - Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] (ab62364)Dot blot analysis of p27 KIP 1 (pS10) phospho peptide (Lane 1) and p27 KIP 1 non-phospho peptide (Lane 2) using ab62364 at 1/1000 dilution followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution.
Blocking and Diluting buffer and concentration: 5% NFDM /TBST.
Exposure time: 3 minutes.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (41)
ab62364 被引用在 41 文献中.
- Hamdin CD et al. Dual-Specificity Phosphatase 6 Deficiency Attenuates Arterial-Injury-Induced Intimal Hyperplasia in Mice. Int J Mol Sci 24:N/A (2023). PubMed: 38138967
- Wu Q et al. Lnc-hipk1 inhibits mouse adipocyte apoptosis as a sponge of miR-497. Biofactors 48:135-147 (2022). PubMed: 34856026
- Zhou H et al. LRP5 regulates cardiomyocyte proliferation and neonatal heart regeneration by the AKT/P21 pathway. J Cell Mol Med 26:2981-2994 (2022). PubMed: 35429093
- Yu Z et al. Protective effect of hepatocyte-enriched lncRNA-Mir122hg by promoting hepatocyte proliferation in acute liver injury. Exp Mol Med 54:2022-2035 (2022). PubMed: 36424455
- Yuan P et al. Proteomics reveals the potential mechanism of Tanshinone IIA in promoting the Ex Vivo expansion of human bone marrow mesenchymal stem cells. Regen Ther 21:560-573 (2022). PubMed: 36475023