重组Anti-p23抗体[EPR3846] (ab92503)
![Western blot - Anti-p23 antibody [EPR3846] (ab92503)](https://www.abcam.cn/ps/products/92/ab92503/Images/ab92503-412707-anti-p23-antibody-epr3846-western-blot-human-knockout-lysate.jpg "Western blot - Anti-p23 antibody [EPR3846] (ab92503)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3846] to p23
- Suitable for: WB, ICC/IF
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-p23抗体[EPR3846]
参阅全部 p23 一抗 -
描述
兔单克隆抗体[EPR3846] to p23 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details
不适用于: Flow Cyt or IHC-P -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide within Human p23 aa 50-150. The exact sequence is proprietary.
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阳性对照
- WB: HEK-293T, HeLa, Human spleen, Jurkat and HepG2 cell lysates ICC/IF: HeLa cells
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR3846 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab92503于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
1/10000 - 1/50000. Predicted molecular weight: 19 kDa.
|
| ICC/IF |
1/100 - 1/250.
|
| 说明 |
|---|
|
WB
1/10000 - 1/50000. Predicted molecular weight: 19 kDa. |
|
ICC/IF
1/100 - 1/250. |
应用说明
Is unsuitable for Flow Cyt or IHC-P.
靶标
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功能
Molecular chaperone that localizes to genomic response elements in a hormone-dependent manner and disrupts receptor-mediated transcriptional activation, by promoting disassembly of transcriptional regulatory complexes. -
通路
Lipid metabolism; prostaglandin biosynthesis. -
序列相似性
Belongs to the p23/wos2 family.
Contains 1 CS domain. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 10728 Human
- Entrez Gene: 100043508 Mouse
- Entrez Gene: 100048119 Mouse
- Entrez Gene: 56351 Mouse
- Entrez Gene: 362809 Rat
- Omim: 607061 Human
- SwissProt: Q15185 Human
- SwissProt: Q9R0Q7 Mouse
see all -
别名
- Co chaperone p23 antibody
- cPGES antibody
- Cytosolic prostaglandin E synthase antibody
see all
图片
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Western blot - Anti-p23 antibody [EPR3846] (ab92503)All lanes : Anti-p23 antibody [EPR3846] (ab92503) at 1/1000 dilution
Lane 1 : Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : PTGES3 knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 19 kDa
Observed band size: 23 kDa why is the actual band size different from the predicted?Lanes 1-4: Merged signal (red and green). Green - ab92503 observed at 23 kDa. Red - loading control ab8245 observed at 36 kDa.
ab92503 Anti-p23 antibody [EPR3846] was shown to specifically react with p23 in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266791 (knockout cell lysate ab258151) was used. Wild-type and p23 knockout samples were subjected to SDS-PAGE. ab92503 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-p23 antibody [EPR3846] (ab92503)](https://www.abcam.cn/ps/products/92/ab92503/Images/ab92503-107560-anti-p23-antibody-epr3846-western-blot.jpg)
Western blot - Anti-p23 antibody [EPR3846] (ab92503)All lanes : Anti-p23 antibody [EPR3846] (ab92503) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Human spleen lysate
Lane 3 : Jurkat cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-labeled goat anti-rabbit at 1/2000 dilution
Predicted band size: 19 kDa
Observed band size: 23 kDa why is the actual band size different from the predicted? -
![Immunocytochemistry/ Immunofluorescence - Anti-p23 antibody [EPR3846] (ab92503)](https://www.abcam.cn/ps/products/92/ab92503/Images/ab92503-107559-anti-p23-antibody-epr3846-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-p23 antibody [EPR3846] (ab92503)Immunofluorescent staining of HeLa cells, using ab92503 at a dilution of 1/100. -
![Anti-p23 antibody [EPR3846] (ab92503)](https://www.abcam.cn/ps/products/92/ab92503/Images/ab92503-4-benefits-of-recombinant-antibodies.png)
Anti-p23 antibody [EPR3846] (ab92503)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab92503 被引用在 7 文献中.
- Hu W et al. Silencing DTX3L Inhibits the Progression of Cervical Carcinoma by Regulating PI3K/AKT/mTOR Signaling Pathway. Int J Mol Sci 24:N/A (2023). PubMed: 36614304
- Qi X et al. HDN-1 induces cell differentiation toward apoptosis in promyelocytic leukemia cells depending on its selective effect on client proteins of Hsp90. Toxicol Appl Pharmacol 417:115459 (2021). PubMed: 33609515
- Takahashi N et al. Up-regulation of cytosolic prostaglandin E synthase in fetal-membrane and amniotic prostaglandin E2 accumulation in labor. PLoS One 16:e0250638 (2021). PubMed: 33891661
- Kowalczyk-Zieba I et al. Hypothyroidism Affects Uterine Function via the Modulation of Prostaglandin Signaling. Animals (Basel) 11:N/A (2021). PubMed: 34573602
- Song HH et al. High mobility group box 1 mediates inflammatory response of astrocytes via cyclooxygenase 2/prostaglandin E2 signaling following spinal cord injury. Neural Regen Res 16:1848-1855 (2021). PubMed: 33510092
- Boruszewska D et al. Expression of enzymes involved in the synthesis of prostaglandin E2 in early- and late-cleaved bovine embryos at different stages of preimplantation development. Theriogenology 133:45-55 (2019). PubMed: 31059928
- He Y et al. Ailanthone targets p23 to overcome MDV3100 resistance in castration-resistant prostate cancer. Nat Commun 7:13122 (2016). PubMed: 27959342