JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB235275

Anti-p130抗体

Anti-p130 antibody

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Polyclonal p130 antibody. Suitable for WB and reacts with Human samples.

查看别名

RB2, RBL2, Retinoblastoma-like protein 2, 130 kDa retinoblastoma-associated protein, Retinoblastoma-related protein 2, pRb2, p130, RBR-2

1 Images
Western blot - Anti-p130 antibody (AB235275)
  • WB

Supplier Data

Western blot - Anti-p130 antibody (AB235275)

Human Protein Atlas : Cell line RNA database 22.9 NX = MOLT4.

IP experiment was performed but did not successfully pull-down p130 in MOLT4 lysate. Antibody is not recommended for IP under these conditions.

All lanes:

Western blot - Anti-p130 antibody (ab235275) at 1/1000 dilution

Lane 1:

MOLT4 whole cell lysate at 1 µg

Lane 2:

MOLT4 whole cell lysate at 10 µg

Lane 3:

Human eye tissue at 1 µg

Lane 4:

Human eye tissue at 10 µg

Secondary

All lanes:

Goat anti-rabbit IgG peroxidase conjugated at 1/10000 dilution

Predicted band size: 128 kDa

Observed band size: 125 kDa

false

Exposure time: 15s

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Human

应用

WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

替代产品

Primary Antibodies

AB76234

Anti-p130 antibody [EP2141Y]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p130 antibody [EP2141Y] (AB76234)

  • 0
  • 0
Primary Antibodies

AB76255

Anti-p130 (phospho S672) antibody [EP2126Y]

RabMAb

Immunoprecipitation - Anti-p130 (phospho S672) antibody [EP2126Y] (AB76255)

  • 0
  • 0
Primary Antibodies

AB211928

Anti-p130 (phospho T986) antibody [EPR2389(2)]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-p130 (phospho T986) antibody [EPR2389(2)] (AB211928)

  • 0
  • 0
Primary Antibodies

AB251534

Anti-p130 (phospho T986) antibody [EPR2389(2)] - BSA and Azide free

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-p130 (phospho T986) antibody [EPR2389(2)] - BSA and Azide free (AB251534)

  • 0
  • 0
Primary Antibodies

AB247453

Anti-p130 antibody [EP2141Y] - BSA and Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p130 antibody [EP2141Y] - BSA and Azide free (AB247453)

  • 0
  • 0

搭配使用产品

Primary Antibodies

AB133531

Anti-p57 Kip2 antibody [EP2718(2)]

20ul selling size|BOND RX™ Validated|RabMAb|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p57 Kip2 antibody [EP2718(2)] (AB133531)

  • 5
  • 2
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB4070

Anti-E2F1 antibody [KH95]

KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E2F1 antibody [KH95] (AB4070)

  • 1
  • 1
Primary Antibodies

AB179450

Anti-FOXO1A antibody [EPR12020]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO1A antibody [EPR12020] (AB179450)

  • 0
  • 0
style
left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p>Recommended for human samples only.</p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
style
left-column
style
left-column

性能和储存信息

形式
Liquid
存储溶液
Preservative: 0.01% Sodium azide Constituents: PBS
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
style
left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

P130 also known as RBL2 (retinoblastoma-like 2) is a member of the retinoblastoma (Rb) protein family which includes pRb and p107. This protein has a molecular weight of about 130 kDa. It plays roles in regulating cell cycle by binding to E2F transcription factors consequently suppressing cell-cycle progression from G1 to S phase. p130 is ubiquitously expressed in various tissues but it shows higher levels in tissues with a low proliferation rate.
Biological function summary

The retinoblastoma-like 2 (p130) functions as an important regulator within the cell cycle checkpoint machinery. p130 forms a complex with E2F transcription factors selectively inhibiting their transcriptional activity. This inhibition plays an integral role in controlling cellular proliferation and ensuring that DNA replication does not occur unchecked. It also associates with cyclin-dependent kinase inhibitors further influencing the progression of the cell cycle.

Pathways

P130 is implicated in the control of the G1/S transition within the cell cycle pathway. Its function is closely linked with the Rb/E2F pathway which is critical for the regulation of cell proliferation. p130 collaborates with Rb and p107 within this pathway ensuring proper control of cell cycle transitions and maintenance of cellular growth.

Retinoblastoma-like 2 (p130) shows significant connections to cancer and neurodegenerative disorders. Dysregulation of p130 along with its pathway partners like Rb can lead to unregulated cell growth contributing to oncogenesis. Additionally alterations in p130 function have implications in the development of Alzheimer's disease where cell cycle dysregulation occurs. Understanding p130's roles more deeply could provide insight into therapeutic targets for these conditions.
style
left-column
style
left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

靶点信息

Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation, associates preferentially with E2F5. Binds to cyclins A and E. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. May act as a tumor suppressor.
See full target information RBL2
style
left-column
style
left-column
style
right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com