AB236374

Anti-p114RhoGEF抗体[OTI6D10]

Name: p114RhoGEF抗体[OTI6D10] (ab236374)| Abcam中文官网
Brand: Abcam Limited
SKU: AB236374
Price: 4864 CNY
Availability: InStock

Anti-p114RhoGEF antibody [OTI6D10]

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Mouse Monoclonal p114RhoGEF antibody. Suitable for WB, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ARHGEF18 aa 100-250.

查看别名

KIAA0521, ARHGEF18, Rho guanine nucleotide exchange factor 18, 114 kDa Rho-specific guanine nucleotide exchange factor, Septin-associated RhoGEF, p114-Rho-GEF, p114RhoGEF, SA-RhoGEF

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p114RhoGEF antibody [OTI6D10] (AB236374)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p114RhoGEF antibody [OTI6D10] (AB236374)

Paraffin-embedded human thyroid carcinoma tissue stained for p114RhoGEF with ab236374 at a 1/500 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p114RhoGEF antibody [OTI6D10] (AB236374)

Paraffin-embedded human breast tissue stained for p114RhoGEF with ab236374 at a 1/500 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p114RhoGEF antibody [OTI6D10] (AB236374)

Paraffin-embedded human lymph node tissue stained for p114RhoGEF with ab236374 at a 1/500 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-p114RhoGEF antibody [OTI6D10] (AB236374)

All lanes:

Western blot - Anti-p114RhoGEF antibody [OTI6D10] (ab236374) at 1/500 dilution

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell extracts at 35 µg

Lane 2:

HepG2 (human liver hepatocellular carcinoma cell line) cell extracts at 35 µg

Predicted band size: 131 kDa

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Supplier Data

Western blot - Anti-p114RhoGEF antibody [OTI6D10] (AB236374)

Cells transfected for 48 hours then lysed.

All lanes:

Western blot - Anti-p114RhoGEF antibody [OTI6D10] (ab236374) at 1/2000 dilution

Lane 1:

pCMV6-ENTRY control transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate at 5 µg

Lane 2:

pCMV6-ENTRY p114RhoGEF transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate at 5 µg

Predicted band size: 131 kDa

false

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

OTI6D10

亚型

IgG1

不含载体蛋白

反应种属

Human

应用

IHC-P, WB

applications

免疫原

Recombinant Fragment Protein within Human ARHGEF18 aa 100-250. The exact immunogen used to generate this antibody is proprietary information.

Q6ZSZ5

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification

纯化说明

Purified from cell culture supernatant.

存储溶液

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

P114RhoGEF also known as ARHGEF18 is a guanine nucleotide exchange factor (GEF) specific for Rho GTPases. It functions by facilitating the exchange of GDP for GTP on RhoA a process important for activating RhoA. p114RhoGEF is approximately 114 kDa in mass. It is expressed in various tissues including vascular endothelial cells and neurons indicating a diverse range of functional roles across different systems.

Biological function summary

This protein plays an important role in the regulation of the actin cytoskeleton dynamics and cell migration. p114RhoGEF associates with other proteins to form complexes that regulate cell shape and motility. It binds with G-proteins and other signaling molecules influencing cellular responses to environmental cues such as changes in cell adhesion or polarity.

Pathways

P114RhoGEF is involved in the Rho signaling pathway which is essential for maintaining cell structure and function. Within this pathway it interacts closely with proteins like ZO-1 and Gα12/13 to control stress fiber formation and focal adhesion. It also features in pathways regulating epithelial cell junctions and endothelial barrier integrity which are critical for tissue homeostasis and vascular function.

P114RhoGEF plays a notable role in cancer progression and cardiovascular diseases. Disruptions in its function can lead to aberrant cell migration and invasion factors linked to tumor metastasis. Additionally changes in its activity might influence endothelial barrier function contributing to vascular leakiness associated with inflammatory conditions. Related proteins such as RhoA and ZO-1 also partake in these pathological processes highlighting the significance of p114RhoGEF in health and disease contexts.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Acts as a guanine nucleotide exchange factor (GEF) for RhoA GTPases. Its activation induces formation of actin stress fibers. Also acts as a GEF for RAC1, inducing production of reactive oxygen species (ROS). Does not act as a GEF for CDC42. The G protein beta-gamma (Gbetagamma) subunits of heterotrimeric G proteins act as activators, explaining the integrated effects of LPA and other G-protein coupled receptor agonists on actin stress fiber formation, cell shape change and ROS production. Required for EPB41L4B-mediated regulation of the circumferential actomyosin belt in epithelial cells (PubMed : 22006950).

See full target information ARHGEF18

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Anti-p114RhoGEF抗体[OTI6D10]

Anti-p114RhoGEF antibody [OTI6D10]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p114RhoGEF antibody [OTI6D10] (AB236374)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p114RhoGEF antibody [OTI6D10] (AB236374)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p114RhoGEF antibody [OTI6D10] (AB236374)

Western blot - Anti-p114RhoGEF antibody [OTI6D10] (AB236374)

Western blot - Anti-p114RhoGEF antibody [OTI6D10] (AB236374)

关键信息

宿主种属

克隆

克隆号

亚型

不含载体蛋白

反应种属

应用

免疫原