Anti-p114RhoGEF抗体(ab10152)
Key features and details
- Goat polyclonal to p114RhoGEF
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-p114RhoGEF抗体
参阅全部 p114RhoGEF 一抗 -
描述
山羊多克隆抗体to p114RhoGEF -
宿主
Goat -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide: SAKEDASKEDVIFF, corresponding to C terminal amino acids 1037-1050 of Human p114RhoGEF.
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常规说明
Rho GTPases are GTP binding proteins that regulate a wide spectrum of cellular functions. These cellular processes include cytoskeletal rearrangements, gene transcription and cell growth and motility. Activation of Rho GTPases is under the direct control of guanine nucleotide exchange factors (GEFs). p114RhoGEF is a guanine nucleotide exchange factor and belongs to the Rho GTPase GFE family. Family members share a common feature, a Dbl (DH) homology domain followed by a pleckstrin (PH) homology domain.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Primary antibody说明
Rho GTPases are GTP binding proteins that regulate a wide spectrum of cellular functions. These cellular processes include cytoskeletal rearrangements, gene transcription and cell growth and motility. Activation of Rho GTPases is under the direct control of guanine nucleotide exchange factors (GEFs). p114RhoGEF is a guanine nucleotide exchange factor and belongs to the Rho GTPase GFE family. Family members share a common feature, a Dbl (DH) homology domain followed by a pleckstrin (PH) homology domain. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab10152于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use at an assay dependent concentration.
1 hour primary incubation is recommended for this product. |
| 说明 |
|---|
|
WB
Use at an assay dependent concentration. 1 hour primary incubation is recommended for this product. |
靶标
- Information by UniProt
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数据库链接
- Entrez Gene: 23370 Human
- SwissProt: Q6ZSZ5 Human
- Unigene: 465761 Human
- Unigene: 736818 Human
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别名
- 114 kDa Rho specific guanine nucleotide exchange factor antibody
- 114 kDa Rho-specific guanine nucleotide exchange factor antibody
- ARHGEF 18 antibody
see all
图片
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Western blot - Anti-p114RhoGEF antibody (ab10152)All lanes : Anti-p114RhoGEF antibody (ab10152) at 0.5 µg/ml
Lane 1 : Daudi cell lysate (protein in RIPA buffer)
Lane 2 : Caco-2 cell lysate (protein in RIPA buffer)
Lysates/proteins at 35 µg per lane.Detected by chemiluminescence.
数据表及文件
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Datasheet download
文献 (2)
ab10152 被引用在 2 文献中.
- Acharya BR et al. A Mechanosensitive RhoA Pathway that Protects Epithelia against Acute Tensile Stress. Dev Cell 47:439-452.e6 (2018). PubMed: 30318244
- Tsuji T et al. Involvement of p114-RhoGEF and Lfc in Wnt-3a- and dishevelled-induced RhoA activation and neurite retraction in N1E-115 mouse neuroblastoma cells. Mol Biol Cell 21:3590-600 (2010). PubMed: 20810787
