AB10152

Anti-p114RhoGEF抗体

Name: p114RhoGEF抗体 (ab10152)| Abcam中文官网
Brand: Abcam Limited
SKU: AB10152
Price: 4606 CNY
Availability: InStock

Anti-p114RhoGEF antibody

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(2 Publications)

Goat Polyclonal p114RhoGEF antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human ARHGEF18 aa 1000-1050.

查看别名

KIAA0521, ARHGEF18, Rho guanine nucleotide exchange factor 18, 114 kDa Rho-specific guanine nucleotide exchange factor, Septin-associated RhoGEF, p114-Rho-GEF, p114RhoGEF, SA-RhoGEF

1 Images

Supplier Data

Western blot - Anti-p114RhoGEF antibody (AB10152)

Detected by chemiluminescence.

All lanes:

Western blot - Anti-p114RhoGEF antibody (ab10152) at 0.5 µg/mL

Lane 1:

Daudi cell lysate (protein in RIPA buffer) at 35 µg

Lane 2:

Caco-2 cell lysate (protein in RIPA buffer) at 35 µg

Predicted band size: 131 kDa,50 kDa,77 kDa

false

关键信息

宿主种属

Goat

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Human

应用

applications

免疫原

Synthetic Peptide within Human ARHGEF18 aa 1000-1050. The exact immunogen used to generate this antibody is proprietary information.

Q6ZSZ5

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>" } } }

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产品详情

Rho GTPases are GTP binding proteins that regulate a wide spectrum of cellular functions. These cellular processes include cytoskeletal rearrangements, gene transcription and cell growth and motility. Activation of Rho GTPases is under the direct control of guanine nucleotide exchange factors (GEFs). p114RhoGEF is a guanine nucleotide exchange factor and belongs to the Rho GTPase GFE family. Family members share a common feature, a Dbl (DH) homology domain followed by a pleckstrin (PH) homology domain.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

纯化说明

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

存储溶液

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

P114RhoGEF also known as ARHGEF18 is a guanine nucleotide exchange factor (GEF) specific for Rho GTPases. It functions by facilitating the exchange of GDP for GTP on RhoA a process important for activating RhoA. p114RhoGEF is approximately 114 kDa in mass. It is expressed in various tissues including vascular endothelial cells and neurons indicating a diverse range of functional roles across different systems.

Biological function summary

This protein plays an important role in the regulation of the actin cytoskeleton dynamics and cell migration. p114RhoGEF associates with other proteins to form complexes that regulate cell shape and motility. It binds with G-proteins and other signaling molecules influencing cellular responses to environmental cues such as changes in cell adhesion or polarity.

Pathways

P114RhoGEF is involved in the Rho signaling pathway which is essential for maintaining cell structure and function. Within this pathway it interacts closely with proteins like ZO-1 and Gα12/13 to control stress fiber formation and focal adhesion. It also features in pathways regulating epithelial cell junctions and endothelial barrier integrity which are critical for tissue homeostasis and vascular function.

P114RhoGEF plays a notable role in cancer progression and cardiovascular diseases. Disruptions in its function can lead to aberrant cell migration and invasion factors linked to tumor metastasis. Additionally changes in its activity might influence endothelial barrier function contributing to vascular leakiness associated with inflammatory conditions. Related proteins such as RhoA and ZO-1 also partake in these pathological processes highlighting the significance of p114RhoGEF in health and disease contexts.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Acts as a guanine nucleotide exchange factor (GEF) for RhoA GTPases. Its activation induces formation of actin stress fibers. Also acts as a GEF for RAC1, inducing production of reactive oxygen species (ROS). Does not act as a GEF for CDC42. The G protein beta-gamma (Gbetagamma) subunits of heterotrimeric G proteins act as activators, explaining the integrated effects of LPA and other G-protein coupled receptor agonists on actin stress fiber formation, cell shape change and ROS production. Required for EPB41L4B-mediated regulation of the circumferential actomyosin belt in epithelial cells (PubMed : 22006950).

See full target information ARHGEF18

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Developmental cell 47:439-452.e6 PubMed30318244

2018

A Mechanosensitive RhoA Pathway that Protects Epithelia against Acute Tensile Stress.

Applications

Unspecified application

Species

Unspecified reactive species

Bipul R Acharya,Alexander Nestor-Bergmann,Xuan Liang,Shafali Gupta,Kinga Duszyc,Estelle Gauquelin,Guillermo A Gomez,Srikanth Budnar,Philippe Marcq,Oliver E Jensen,Zev Bryant,Alpha S Yap

Molecular biology of the cell 21:3590-600 PubMed20810787

2010

Involvement of p114-RhoGEF and Lfc in Wnt-3a- and dishevelled-induced RhoA activation and neurite retraction in N1E-115 mouse neuroblastoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Takuji Tsuji,Yusaku Ohta,Yuya Kanno,Kenzo Hirose,Kazumasa Ohashi,Kensaku Mizuno

View all publications

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Anti-p114RhoGEF抗体

Anti-p114RhoGEF antibody

Western blot - Anti-p114RhoGEF antibody (AB10152)