Anti-P Glycoprotein抗体[JSB-1] (ab3366)

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The P glycoprotein antibody ab3366 was used for IHC as shown on the datasheeet with an anti-mouse HRP secondary, then developed with DAB to get the brown color. If you do need an HRP conjugated secondary against mouse, we have many in our catalog including ab98799.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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The concentration for ab3366, lot GR80263 is 67ug/ml.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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For ab3366 (https://www.abcam.com/p-glycoprotein-antibody-jsb-1-ab3366.html), the immunogen is a multidrug-resistant Chinese hamster ovary cell line, so the specific epitope is unknown.

ab3366 is the only anti-p glycoprotein antibody that we carry that has been tested and guaranteed to work in the applications (WB, IP, FC, ICC, ELISA, IHC-P) and species (mouse, rat) that you are interested in.

This antibody is covered by our Abpromise guarantee. We are happy to provide scientific support at anytime. If you are using the product in a species and applications listed on the datasheet and contact us within six months of purchase, we are also happy to replace or refund the product should we not be able to help you to resolve the issue. More information on our Abpromise may be found at the following link:
https://www.abcam.com/index.html?pageconfig=abpromise

Our other anti-p glycoprotein antibodies are listed in the link below:
https://www.abcam.com/index.html?t=11041&pt=1

For untested species or applications, we have established a testing discount program. Here is a brief description of how it works:

The testing discount program is for customers who like to use an antibody/protein on an untested species/application. You would purchase the antibody at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount equal to the value of the product tested. The terms and conditions of this offer can be found here: https://www.abcam.com/AbTrial

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

Thank you for passing on that reply.

I am sorry to hear that the customer has still not observed the expected signal with the anti-P Glycoprotein antibody (ab3366).

As I mentioned in my previous email, I would be happy to provide the customer with a different antibody to try. I would suggest that they have a look at the rabbit polyclonal ab129450 or the mouse monoclonal ab80594. Both of these have been used previously and shown to detect the P Glycoprotein in Western blotting.

The use of the mouse monoclonal ab80594 has been described in the following references:

Chu TM et al. Detection of soluble P-glycoprotein in culture media and extracellular fluids. Biochem Biophys Res Commun 203:506-12 (1994). PubMed: 7915520
Chu TM et al. Characterization of a new monoclonal antibody F4 detecting cell surface epitope and P-glycoprotein in drug-resistant human tumor cell lines. Hybridoma 12:417-29 (1993). PubMed: 7503940



Whilst the results observed with the rabbit polyclonal ab129450 can be observed on the datasheet of the antibody:

https://www.abcam.com/ab129450

Although the Western blot for ab129450 is picking up some background, the band of interest is observed strongly and it may well be that with further optimisation of the blocking conditions etc. that the blot would look much cleaner.

Both of these antibodies would again be covered by the Abpromise guarantee were the customer to have any further problems.

I look forward to receiving your reply.

Thank you for taking time to complete our questionnaire and for contacting us. I am sorry to hear this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality. I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been successful.

I have been in contact with the lab concerning this complaint and they have suggested that the antibody may need to be used at a higher concentration in order to see a signal in your Western blot. It may therefore be worthwhile to increase the concentration to 1/10 in order to see if a signal is detected.

However, I can understand your concerns and appreciate the time you have spent in trying to optimise this antibody. If you would like, I can provide you with a different antibody such as the rabbit polyclonal ab129450 or the mouse monoclonal ab80594 to try? Alternatively, if you would prefer, I can arrange for a credit note to be issued.

Thank you for your cooperation. I look forward to hearing from you with details of how you would like to proceed.

Thank you for contacting us.

The concentration of this product is 67ug/ml.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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https://www.abcam.com/abreviews

Thanks for your reply.

I can confirm this is the same batch number. The full number is 1396975 GR87957-2.

I hope this will be helpful. Please do not hesitate to let me know if you need anything further.

Thank you for your enquiry,

I can confirm that the concentration of P Glycoprotein antibody [JSB-1] is lot dependent.

I am sorry I am not sure which batch number you have. The last order we sent to Turkey of this antibody had lot number 1396975 . This has a concentration of 67 ug/ml.

I hope this will be helpful to you. If you check the vial and find you have a different lot number, please do not hesitate to let me know and I will be pleased to find the relevant concentration for you.

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement of ab3377 with the order number xxxx. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.