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Signal Transduction Metabolism Plasma Membrane Channels

Anti-P Glycoprotein抗体[JSB-1] (ab3366)

  • Datasheet
  • SDS
Reviews (1)Q&A (52)References (43)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [JSB-1] (ab3366)

    Key features and details

    • Mouse monoclonal [JSB-1] to P Glycoprotein
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG1

    选择批间可重复性更高的重组抗体

    Product image
    Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)
    • 研究可靠 —— 各批次间结果一致且可重复
    • 长期批量供应 —— 采用重组技术,可实现快速生产
    • 首次实验即可成功 —— 经过大量验证确认了特异性
    • 符合伦理标准 —— 产品不含动物成分

    概述

    • 产品名称

      Anti-P Glycoprotein抗体[JSB-1]
      参阅全部 P Glycoprotein 一抗
    • 描述

      小鼠单克隆抗体[JSB-1] to P Glycoprotein
    • 宿主

      Mouse
    • 特异性

      This antibody is specific for the MDR1 isoform of P Glycoprotein which is only found in the plasma membrane. It does not cross-react with MDR3. It has been shown to cross-react with Pyruvate Carboxylase, an abundant mitochondrial enzyme (130 kDa), on both immunoblots and immunohistochemical tissue sections. Weak homogeneous, cytoplasmic, or granular patterns of reactivity may represent staining of the PC cross-reactive epitope rather than positive staining for P glycoprotein.
    • 经测试应用

      适用于: IHC-Pmore details
    • 种属反应性

      与反应: Human
    • 免疫原

      Multidrug-resistant Chinese hamster ovary cell line (CHrC5).

    • 表位

      Cytoplasmic domain present on the MDR1 isoform of P Glycoprotein. This epitope is strongly conserved in mammals.
    • 阳性对照

      • Drug-sensitive parental cell lines and their multidrug-resistant derivatives. Human liver (positive staining detected along luminal surfaces of bilecanaliculi) or human colon (positive staining localized to luminal surface of secretaoryepithelium).
    • 常规说明

      This antibody is reported to work in WB according to the literature(PubMed 22389470 and 17961285). Due to customer feedback of difficulty in WB, we are no longer able to guarantee ab3366 in WB.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • 存储溶液

      Preservative: 0.1% Sodium azide
      Constituents: PBS, 1% BSA
    • Concentration information loading...
    • 纯度

      Tissue culture supernatant
    • 克隆

      单克隆
    • 克隆编号

      JSB-1
    • 同种型

      IgG1
    • 研究领域

      • Signal Transduction
      • Metabolism
      • Plasma Membrane
      • Channels
      • Stem Cells
      • Hematopoietic Progenitors
      • Surface Molecules
      • Cancer
      • Drug resistance
      • P glycoproteins
      • Metabolism
      • Types of disease
      • Cancer
      • Neuroscience
      • Processes

    相关产品

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Isotype control

      • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab3366于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    IHC-P (1)
    1/40. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (EDTA, pH 8-9 for example). 20-60 minutes at room temperature.
    说明
    IHC-P
    1/40. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (EDTA, pH 8-9 for example). 20-60 minutes at room temperature.

    靶标

    • 功能

      Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells.
    • 组织特异性

      Expressed in liver, kidney, small intestine and brain.
    • 疾病相关

      Genetic variations in ABCB1 are associated with susceptibility to inflammatory bowel disease type 13 (IBD13) [MIM:612244]. Inflammatory bowel disease is characterized by a chronic relapsing intestinal inflammation. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may involve any part of the gastrointestinal tract, but most frequently the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints. Crohn disease and ulcerative colitis are commonly classified as autoimmune diseases.
    • 序列相似性

      Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.
      Contains 2 ABC transmembrane type-1 domains.
      Contains 2 ABC transporter domains.
    • 细胞定位

      Membrane.
    • Target information above from: UniProt accession P08183 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 5243 Human
      • Omim: 171050 Human
      • SwissProt: P08183 Human
      • Unigene: 489033 Human
      • 别名

        • ABC20 antibody
        • ABCB1 antibody
        • ATP binding cassette, sub family B (MDR/TAP), member 1 antibody
        • ATP-binding cassette sub-family B member 1 antibody
        • CD243 antibody
        • CLCS antibody
        • Colchicin sensitivity antibody
        • Doxorubicin resistance antibody
        • GP170 antibody
        • MDR1 antibody
        • MDR1_HUMAN antibody
        • Multidrug resistance 1 antibody
        • Multidrug resistance protein 1 antibody
        • P glycoprotein 1 antibody
        • P gp antibody
        • P-glycoprotein 1 antibody
        • PGY1 antibody
        see all

      图片

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [JSB-1] (ab3366)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [JSB-1] (ab3366)
        ab3366 (4µg/ml) staining p Glycoprotein in human liver, using an automated system (DAKO Autostainer Plus). Using this protocol there is a cell membrane staining of a subpopulation of hepatocytes.
        Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

      实验方案

      • Immunohistochemistry protocols

      Click here to view the general protocols

      数据表及文件

      • SDS download

      • Datasheet download

        Download

      文献 (43)

      发表研究结果有使用 ab3366?请让我们知道,以便我们可以引用本数据表中的参考文章。

      ab3366 被引用在 43 文献中.

      • Niu Y  et al. Interaction of Hepatitis B Virus X Protein with the Pregnane X Receptor Enhances the Synergistic Effects of Aflatoxin B1 and Hepatitis B Virus on Promoting Hepatocarcinogenesis. J Clin Transl Hepatol 9:466-476 (2021). PubMed: 34447675
      • Vita SM  et al. P-glycoprotein Expression Is Upregulated in a Pre-Clinical Model of Traumatic Brain Injury. Neurotrauma Rep 1:207-217 (2020). PubMed: 33274346
      • Chen L  et al. Everolimus Reverses Palbociclib Resistance in ER+ Human Breast Cancer Cells by Inhibiting Phosphatidylinositol 3-Kinase(PI3K)/Akt/Mammalian Target of Rapamycin (mTOR) Pathway. Med Sci Monit 25:77-86 (2019). PubMed: 30605443
      • Koehn LM  et al. Developmental differences in the expression of ABC transporters at rat brain barrier interfaces following chronic exposure to diallyl sulfide. Sci Rep 9:5998 (2019). PubMed: 30979952
      • Hasanovic A  et al. Targeting the multidrug transporter Patched potentiates chemotherapy efficiency on adrenocortical carcinoma in vitro and in vivo. Int J Cancer 143:199-211 (2018). PubMed: 29411361
      View all Publications for this product

      客户评价及客户问答

      Show All 评价 Q&A
      提交评价 提交问题

      1-10 of 53 Abreviews or Q&A

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-P Glycoprotein antibody [JSB-1]

      Average
      Abreviews
      Abreviews
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Human Tissue sections (liver)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: cell conditioner 1
      Permeabilization
      No
      Specification
      liver
      Fixative
      Formaldehyde
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 Apr 08 2019

      Question

      what was used to develop in this image

      Read More

      Abcam community

      Verified customer

      Asked on Mar 05 2013

      Answer

      Thank you for contacting us.

      The P glycoprotein antibody ab3366 was used for IHC as shown on the datasheeet with an anti-mouse HRP secondary, then developed with DAB to get the brown color. If you do need an HRP conjugated secondary against mouse, we have many in our catalog including ab98799.

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Read More

      Abcam Scientific Support

      回复于 Mar 05 2013

      Question

      Inquiry: What is the concentration of the antibody ab3366, Lot N. GR80263? Thanks

      Read More

      Abcam community

      Verified customer

      Asked on Dec 13 2012

      Answer

      Thank you for contacting us.
      The concentration for ab3366, lot GR80263 is 67ug/ml.
      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
      Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

      Read More

      Abcam Scientific Support

      回复于 Dec 13 2012

      Question

      Customer kindly contacted us regarding P Glycoprotein antibodies for use in WB, IP, microscopy, FC in mouse cells and rat tissue. Customer inquiring about more specific immunogen information.

      Read More

      Abcam community

      Verified customer

      Asked on Dec 05 2012

      Answer

      Thank you for contacting Abcam.

      For ab3366 (https://www.abcam.com/p-glycoprotein-antibody-jsb-1-ab3366.html), the immunogen is a multidrug-resistant Chinese hamster ovary cell line, so the specific epitope is unknown.

      ab3366 is the only anti-p glycoprotein antibody that we carry that has been tested and guaranteed to work in the applications (WB, IP, FC, ICC, ELISA, IHC-P) and species (mouse, rat) that you are interested in.

      This antibody is covered by our Abpromise guarantee. We are happy to provide scientific support at anytime. If you are using the product in a species and applications listed on the datasheet and contact us within six months of purchase, we are also happy to replace or refund the product should we not be able to help you to resolve the issue. More information on our Abpromise may be found at the following link:
      https://www.abcam.com/index.html?pageconfig=abpromise

      Our other anti-p glycoprotein antibodies are listed in the link below:
      https://www.abcam.com/index.html?t=11041&pt=1

      For untested species or applications, we have established a testing discount program. Here is a brief description of how it works:

      The testing discount program is for customers who like to use an antibody/protein on an untested species/application. You would purchase the antibody at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount equal to the value of the product tested. The terms and conditions of this offer can be found here: https://www.abcam.com/AbTrial

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

      Read More

      Abcam Scientific Support

      回复于 Dec 05 2012

      Question

      Hi,

      I hope you have been well

      Our customer has responded with the following;

      There was a conference last week so apologies for the delay). xxxxx (student) tried a western blot last week using a primary ABCB1 antibody concentration of 1:50. It’s not possible to use 1:10 as there’s only 250uL antibody and we need 3mL for the blot. Again there was absolutely nothing, so we’d like to take up the offer of trying a different antibody to the same protein – whichever one Abcam feels would work best. I’ve been in contact with someone who works on ABC transporters and she’s said that the positive control that we have is the best one to use (they have their own antibody, so she had no recommendations of a good commercial antibody!).

      Thanks very much for your help.

      Hoping to hear back from you soon.

      Read More

      Abcam community

      Verified customer

      Asked on Dec 05 2012

      Answer

      Thank you for passing on that reply.

      I am sorry to hear that the customer has still not observed the expected signal with the anti-P Glycoprotein antibody (ab3366).

      As I mentioned in my previous email, I would be happy to provide the customer with a different antibody to try. I would suggest that they have a look at the rabbit polyclonal ab129450 or the mouse monoclonal ab80594. Both of these have been used previously and shown to detect the P Glycoprotein in Western blotting.

      The use of the mouse monoclonal ab80594 has been described in the following references:

      Chu TM et al. Detection of soluble P-glycoprotein in culture media and extracellular fluids. Biochem Biophys Res Commun 203:506-12 (1994). PubMed: 7915520
      Chu TM et al. Characterization of a new monoclonal antibody F4 detecting cell surface epitope and P-glycoprotein in drug-resistant human tumor cell lines. Hybridoma 12:417-29 (1993). PubMed: 7503940



      Whilst the results observed with the rabbit polyclonal ab129450 can be observed on the datasheet of the antibody:

      https://www.abcam.com/ab129450

      Although the Western blot for ab129450 is picking up some background, the band of interest is observed strongly and it may well be that with further optimisation of the blocking conditions etc. that the blot would look much cleaner.

      Both of these antibodies would again be covered by the Abpromise guarantee were the customer to have any further problems.

      I look forward to receiving your reply.

      Read More

      Abcam Scientific Support

      回复于 Dec 05 2012

      Question

      Product code: 3366
      Lot number: GR87957-1

      Inquiry: Hi Technical, We have a customer who is experiencing problems with this antibody. Kindly find attached a questionnaire that the customer completed. Our xxxx dated 19 Sep 2012.

      Read More

      Abcam community

      Verified customer

      Asked on Nov 20 2012

      Answer

      Thank you for taking time to complete our questionnaire and for contacting us. I am sorry to hear this antibody is not providing satisfactory results.

      The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality. I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been successful.

      I have been in contact with the lab concerning this complaint and they have suggested that the antibody may need to be used at a higher concentration in order to see a signal in your Western blot. It may therefore be worthwhile to increase the concentration to 1/10 in order to see if a signal is detected.

      However, I can understand your concerns and appreciate the time you have spent in trying to optimise this antibody. If you would like, I can provide you with a different antibody such as the rabbit polyclonal ab129450 or the mouse monoclonal ab80594 to try? Alternatively, if you would prefer, I can arrange for a credit note to be issued.

      Thank you for your cooperation. I look forward to hearing from you with details of how you would like to proceed.

      Read More

      Abcam Scientific Support

      回复于 Nov 20 2012

      Question

      We have ordered Anti-P Glycoprotein antibody [JSB-1] (ab3366) and received
      it through Abcam's official representative in Turkey, namely from Kimera
      Medical Laboratory Supplies.
      We would like to learn the concentration of the product. It is only
      written 250 ul for the concentration of this product on Abcam's web-site
      (https://www.abcam.com/P-Glycoprotein-antibody-JSB-1-ab3366.html).
      We would be glad if you could inform us on the concentration of this product.
      Sincerely,

      Read More

      Abcam community

      Verified customer

      Asked on Nov 01 2012

      Answer

      Thank you for contacting us.

      The concentration of this product is 67ug/ml.

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Use our products? Submit an Abreview. Earn rewards!
      https://www.abcam.com/abreviews

      Read More

      Abcam Scientific Support

      回复于 Nov 01 2012

      Question


      Thanks for your prompt response. Lot of the product that we have is
      different. It is GR87957-2. Is it possible for you to specify the
      concentration of P Glycoprotein antibody [JSB-1] with this lot number?
      Thanks in advance.
      Best regards,

      Read More

      Abcam community

      Verified customer

      Asked on Oct 31 2012

      Answer

      Thanks for your reply.

      I can confirm this is the same batch number. The full number is 1396975 GR87957-2.

      I hope this will be helpful. Please do not hesitate to let me know if you need anything further.

      Read More

      Abcam Scientific Support

      回复于 Oct 31 2012

      Question

      Dear Abcam Member,
      We have ordered Anti-P Glycoprotein antibody [JSB-1] (ab3366) and received
      it through Abcam's official representative in Turkey, namely from Kimera
      Medical Laboratory Supplies.
      We would like to learn the concentration of the product. It is only
      written 250 ul for the concentration of this product on Abcam's web-site
      (https://www.abcam.com/P-Glycoprotein-antibody-JSB-1-ab3366.html).
      We would be glad if you could inform us on the concentration of this product.
      Sincerely,

      Read More

      Abcam community

      Verified customer

      Asked on Oct 31 2012

      Answer

      Thank you for your enquiry,

      I can confirm that the concentration of P Glycoprotein antibody [JSB-1] is lot dependent.

      I am sorry I am not sure which batch number you have. The last order we sent to Turkey of this antibody had lot number 1396975 . This has a concentration of 67 ug/ml.

      I hope this will be helpful to you. If you check the vial and find you have a different lot number, please do not hesitate to let me know and I will be pleased to find the relevant concentration for you.

      Read More

      Abcam Scientific Support

      回复于 Oct 31 2012

      Question

      xxxx is the PO number that was used to purchase the antibody ab3366.  Please let me know if you need any more details.  Thanks!

      Read More

      Abcam community

      Verified customer

      Asked on Oct 29 2012

      Answer

      Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement of ab3377 with the order number xxxx. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.

      Read More

      Abcam Scientific Support

      回复于 Oct 29 2012

      1-10 of 53 Abreviews or Q&A

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