Anti-P Glycoprotein抗体[JSB-1] (ab3366)
Key features and details
- Mouse monoclonal [JSB-1] to P Glycoprotein
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG1
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-P Glycoprotein抗体[JSB-1]
参阅全部 P Glycoprotein 一抗 -
描述
小鼠单克隆抗体[JSB-1] to P Glycoprotein -
宿主
Mouse -
特异性
This antibody is specific for the MDR1 isoform of P Glycoprotein which is only found in the plasma membrane. It does not cross-react with MDR3. It has been shown to cross-react with Pyruvate Carboxylase, an abundant mitochondrial enzyme (130 kDa), on both immunoblots and immunohistochemical tissue sections. Weak homogeneous, cytoplasmic, or granular patterns of reactivity may represent staining of the PC cross-reactive epitope rather than positive staining for P glycoprotein. -
经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Multidrug-resistant Chinese hamster ovary cell line (CHrC5).
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表位
Cytoplasmic domain present on the MDR1 isoform of P Glycoprotein. This epitope is strongly conserved in mammals. -
阳性对照
- Drug-sensitive parental cell lines and their multidrug-resistant derivatives. Human liver (positive staining detected along luminal surfaces of bilecanaliculi) or human colon (positive staining localized to luminal surface of secretaoryepithelium).
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常规说明
This antibody is reported to work in WB according to the literature(PubMed 22389470 and 17961285). Due to customer feedback of difficulty in WB, we are no longer able to guarantee ab3366 in WB.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
JSB-1 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P | (1) |
1/40. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (EDTA, pH 8-9 for example). 20-60 minutes at room temperature.
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说明 |
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IHC-P
1/40. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (EDTA, pH 8-9 for example). 20-60 minutes at room temperature. |
靶标
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功能
Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells. -
组织特异性
Expressed in liver, kidney, small intestine and brain. -
疾病相关
Genetic variations in ABCB1 are associated with susceptibility to inflammatory bowel disease type 13 (IBD13) [MIM:612244]. Inflammatory bowel disease is characterized by a chronic relapsing intestinal inflammation. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may involve any part of the gastrointestinal tract, but most frequently the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints. Crohn disease and ulcerative colitis are commonly classified as autoimmune diseases. -
序列相似性
Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.
Contains 2 ABC transmembrane type-1 domains.
Contains 2 ABC transporter domains. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 5243 Human
- Omim: 171050 Human
- SwissProt: P08183 Human
- Unigene: 489033 Human
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别名
- ABC20 antibody
- ABCB1 antibody
- ATP binding cassette, sub family B (MDR/TAP), member 1 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [JSB-1] (ab3366)ab3366 (4µg/ml) staining p Glycoprotein in human liver, using an automated system (DAKO Autostainer Plus). Using this protocol there is a cell membrane staining of a subpopulation of hepatocytes.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
数据表及文件
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SDS download
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Datasheet download
文献 (45)
ab3366 被引用在 45 文献中.
- Niu Y et al. Interaction of Hepatitis B Virus X Protein with the Pregnane X Receptor Enhances the Synergistic Effects of Aflatoxin B1 and Hepatitis B Virus on Promoting Hepatocarcinogenesis. J Clin Transl Hepatol 9:466-476 (2021). PubMed: 34447675
- Jin R et al. MRI-Visible Nanovehicle for Efficient siRNA Delivery. Methods Mol Biol 2282:195-208 (2021). PubMed: 33928578
- Vita SM et al. P-glycoprotein Expression Is Upregulated in a Pre-Clinical Model of Traumatic Brain Injury. Neurotrauma Rep 1:207-217 (2020). PubMed: 33274346
- Chen L et al. Everolimus Reverses Palbociclib Resistance in ER+ Human Breast Cancer Cells by Inhibiting Phosphatidylinositol 3-Kinase(PI3K)/Akt/Mammalian Target of Rapamycin (mTOR) Pathway. Med Sci Monit 25:77-86 (2019). PubMed: 30605443
- Koehn LM et al. Developmental differences in the expression of ABC transporters at rat brain barrier interfaces following chronic exposure to diallyl sulfide. Sci Rep 9:5998 (2019). PubMed: 30979952