Anti-OTUB1抗体(ab82154)
Key features and details
- Goat polyclonal to OTUB1
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
概述
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产品名称
Anti-OTUB1抗体
参阅全部 OTUB1 一抗 -
描述
山羊多克隆抗体to OTUB1 -
宿主
Goat -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse
预测可用于: Rat, Cow, Cynomolgus monkey
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免疫原
Synthetic peptide: C-
YKEYAEDDNIYQQK
corresponding to internal sequence amino acids 58-71 of human OTUB1 (NP_060140.2). -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab82154于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (2) |
Use a concentration of 0.03 - 0.1 µg/ml. Predicted molecular weight: 31 kDa.
1 hour primary incubation is recommended for this product. |
| 说明 |
|---|
|
WB
Use a concentration of 0.03 - 0.1 µg/ml. Predicted molecular weight: 31 kDa. 1 hour primary incubation is recommended for this product. |
靶标
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功能
Hydrolase that can remove conjugated ubiquitin from proteins and plays an important regulatory role at the level of protein turnover by preventing degradation. Regulator of T-cell anergy, a phenomenon that occurs when T-cells are rendered unresponsive to antigen rechallenge and no longer respond to their cognate antigen. Acts via its interaction with RNF128/GRAIL, a crucial inductor of CD4 T-cell anergy. Isoform 1 destabilizes RNF128, leading to prevent anergy. In contrast, isoform 2 stabilizes RNF128 and promotes anergy. Surprisingly, it regulates RNF128-mediated ubiquitination, but does not deubiquitinate polyubiquitinated RNF128. Deubiquitinates estrogen receptor alpha (ESR1). Mediates deubiquitination of 'Lys-48'-linked polyubiquitin chains, but not 'Lys-63'-linked polyubiquitin chains. Not able to cleave di-ubiquitin. Also capable of removing NEDD8 from NEDD8 conjugates, but with a nuch lower preference compared to 'Lys-48'-linked ubiquitin. -
组织特异性
Isoform 1 is ubiquitous. Isoform 2 is expressed only in lymphoid tissues such as tonsils, lymph nodes and spleen, as well as peripheral blood mononuclear cells. -
序列相似性
Belongs to the peptidase C65 family.
Contains 1 OTU domain. -
结构域
In addition to ubiquitin-binding at the Cys-91 active site, a proximal ubiquitin-binding site is also present at Cys-23 Occupancy of the active site is needed to enable tight binding to the second site. Distinct binding sites for the ubiquitins may allow to discriminate among different isopeptide linkages (i.e. 'Lys-48'-, 'Lys-63'-linked polyubiquitin) in polyubiquitin substrates and achieve linkage-specific deubiquitination. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 540984 Cow
- Entrez Gene: 107260 Mouse
- Entrez Gene: 293705 Rat
- SwissProt: Q7TQI3 Mouse
- SwissProt: B2RYG6 Rat
- Unigene: 203921 Mouse
- Unigene: 9218 Rat
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别名
- Deubiquitinating enzyme OTUB1 antibody
- hOTU1 antibody
- HSPC263 antibody
see all
图片
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Western blot - Anti-OTUB1 antibody (ab82154)Image courtesy of an Anonymous review
Anti-OTUB1 antibody (ab82154) reactivity with reduced HEK293 cell lysate after transient transfection (48h) of control siRNA (lane 1-3) or siRNA targeting OTUB1 (lane 4 and 5). After SDS-PAGE, membranes were blocked in 5% milk TBS + 0.1% Tween for 1h at 25°C before incubation with ab82154 (1:1000 dilution in 5% milk TBS + 0.1% Tween) for 16h at 4ºC. Blots was then incubated with an anti-Goat HRP-conjugated secondary antibody before developing with ECL. Anti-tubulin blot included as a loading control.
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Western blot - Anti-OTUB1 antibody (ab82154)Anti-OTUB1 antibody (ab82154) at 0.5 µg/ml + mouse brain lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 31 kDa
Observed band size: 31 kDa
Exposure time: 1 hour
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab82154 尚未被引用在任何文献中。