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Anti-Otoferlin抗体[13A9] (ab53233)

Reviews (3)Q&A (4)References (33)
Flow Cytometry - Anti-Otoferlin antibody [13A9] (ab53233)
  • Immunocytochemistry/ Immunofluorescence - Anti-Otoferlin antibody [13A9] (ab53233)

Key features and details

  • Mouse monoclonal [13A9] to Otoferlin
  • Suitable for: Flow Cyt, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG1

概述

  • 产品名称

    Anti-Otoferlin抗体[13A9]
    参阅全部 Otoferlin 一抗

  • 描述

    小鼠单克隆抗体[13A9] to Otoferlin

  • 宿主

    Mouse

  • 特异性

    This antibody reacts specifically with human Otoferlin protein (220 kDa).

  • 经测试应用

    适用于: Flow Cyt, ICC/IFmore details

  • 种属反应性

    与反应: Mouse, Human

  • 免疫原

    Fusion protein corresponding to Human Otoferlin aa 1-400.
    Database link: Q9HC10

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab53233于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF (1)
1/100 - 1/500.
说明
Flow Cyt
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF
1/100 - 1/500.

靶标

  • 功能

    Key calcium ion sensor involved in the Ca(2+)-triggered synaptic vesicle-plasma membrane fusion and in the control of neurotransmitter release at these output synapses. Interacts in a calcium-dependent manner to the presynaptic SNARE proteins at ribbon synapses of cochlear inner hair cells (IHCs) to trigger exocytosis of neurotransmitter. Also essential to synaptic exocytosis in immature outer hair cells (OHCs). May also play a role within the recycling of endosomes.

  • 组织特异性

    Isoform 1 and isoform 3 are found in adult brain. Isoform 2 is expressed in the fetus and in adult brain, heart, placenta, skeletal muscle and kidney.

  • 疾病相关

    Defects in OTOF are the cause of deafness autosomal recessive type 9 (DFNB9) [MIM:601071]. DFNB9 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.
    Defects in OTOF are a cause of non-syndromic auditory neuropathy autosomal recessive (NSRAN) [MIM:601071]. NSRAN is a form of sensorineural hearing impairment with absent or severely abnormal auditory brainstem response but normal otoacoustic emissions. Auditory neuropathies result from a lesion in the area including the inner hair cells, connections between the inner hair cells and the cochlear branch of the auditory nerve, the auditory nerve itself and auditory pathways of the brainstem. In some cases NSRAN phenotype can be temperature sensitive.

  • 序列相似性

    Belongs to the ferlin family.
    Contains 4 C2 domains.

  • 细胞定位

    Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Basolateral cell membrane. Endoplasmic reticulum membrane. Cell membrane. Detected at basolateral cell membrane with synaptic vesicles surrounding the ribbon and at the presynaptic plasma membrane in the inner hair cells (IHCs). Colocalizes with GPR25 and RAB8B in inner hair cells.
  • Information by UniProt

  • 数据库链接

    • 别名

      • AUNB1 antibody
      • Deafness, autosomal recessive 9 antibody
      • DFNB6 antibody
      • DFNB9 antibody
      • Fer 1 like protein 2 antibody
      • Fer-1-like protein 2 antibody
      • FER1L2 antibody
      • NSRD9 antibody
      • Otof antibody
      • OTOF_HUMAN antibody
      • Otoferlin antibody
      see all

    图片

    • Flow Cytometry - Anti-Otoferlin antibody [13A9] (ab53233)
      Flow Cytometry - Anti-Otoferlin antibody [13A9] (ab53233)
      Overlay histogram showing SHSY-5Y cells stained with ab53233 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab53233, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SHSY-5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    • Immunocytochemistry/ Immunofluorescence - Anti-Otoferlin antibody [13A9] (ab53233)
      Immunocytochemistry/ Immunofluorescence - Anti-Otoferlin antibody [13A9] (ab53233)

      ICC/IF analysis of mouse inner ear tissue labeling Otoferlin with ab181781. At 30 days postnatal, Otoferlin is detected in the hair cells of the crista ampullaris (CA) , utricular macula (UM) and saccularmacula (SM).

    文献 (33)

    发表研究结果有使用 ab53233？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab53233 被引用在 33 文献中.

    • Ikäheimo K  et al. MANF supports the inner hair cell synapse and the outer hair cell stereocilia bundle in the cochlea. Life Sci Alliance 5:N/A (2022). PubMed: 34815294
    • Guo R  et al. Complete Elimination of Peripheral Auditory Input Before Onset of Hearing Causes Long-Lasting Impaired Social Memory in Mice. Front Neurosci 15:723658 (2021). PubMed: 34385906
    • Wang G  et al. Mosaic CRISPR-stop enables rapid phenotyping of nonsense mutations in essential genes. Development 148:N/A (2021). PubMed: 33558388
    • Hosoya M  et al. Neuronal development in the cochlea of a nonhuman primate model, the common marmoset. Dev Neurobiol 81:905-938 (2021). PubMed: 34545999
    • Kroll J  et al. AP180 promotes release site clearance and clathrin-dependent vesicle reformation in mouse cochlear inner hair cells. J Cell Sci 133:N/A (2020). PubMed: 31843760
    View all Publications for this product

    客户评价及客户问答

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    提交评价 提交问题

    1-7 of 7 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Otoferlin antibody [13A9]

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Head)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris-EDTA buffer pH 7.8
    Permeabilization
    No
    Specification
    Head
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 23°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Aug 24 2018

    IHC - Wholemount abreview for Anti-Otoferlin antibody [13A9]

     Inconclusive
    Abreviews
    abreview image
    Application
    IHC - Wholemount
    Sample
    (Paddlefish) Polyodon spathula Tissue (hair cells in otic vesicle)
    Specification
    hair cells in otic vesicle
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jun 22 2014

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Otoferlin antibody [13A9]

     Good
    Abreviews
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Zebrafish Cell (Whole organism)
    Specification
    Whole organism
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - triton
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Colin Johnson

    Verified customer

    提交于 Jun 20 2012

    Question

    vielen Dank für die Info bezüglich des webinars zum Thema Immunocytochemistry.
    Hätte auf jeden auch Interesse an einem Immunohisto webinars. bzw. uns ist ein Buch empfohlen worden :
    'Immunohistochemistry' (edited by S. Renshaw), ISBN: 978-1-904842-03-3.
    Hätten Sie das noch auf Lager?
    Könnten dann nämlich unseren OTOferlin Ak ab53233 und den Cy3 donkey anti rabbit den wir von Ihnen haben nochmal ausprobieren.Beim 1.Mal ist etwas schief gegangen und die Fbg. war leider nicht so gut geworden.

    Read More
    Answer

    Vielen Dank fürIhre Anfrage.

    Ein IHC Webinar wurde bereits im Juni 2011 gehalten. Sie koennen es sich auf unserer Webseite unter https://www.abcam.com/blog/ oder https://www.abcam.com/blog/index.cfm/Webinars anschauen.

    Das Buch "Immunohistochemistry" ist verfuegbar als ab94964 fuer ein Preis von €50.00 + Lieferkosten.

    Falls Sie weitere Fragen haben oder der Antikoerper ab53233 Ihnen weiterhin Probleme bereitet, sagen Sie mir bitte Bescheid.

    Read More

    Question

    Hi,
    I would like to take yo up on your offer to purchase and try the otoferlin antibody on zebrafish and obtain the discount code.

    Read More
    Answer

    DISCOUNT CODE: x


    Expiration date: dd mm 2012

    I am very pleased to hear you would like to accept our offer and test ab53233 in zebrafish. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for zebrafish and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount

    Read More

    Question

    Hello,
    We are interested in a anti-otoferlin antibody for use in immunohistochemistry in z
    ebrafish. Do you know if your anti-otoferlin monoclonal ab53233 works in zebrafish? I
    f not, would it be possible to test a small sample on our zebrafish tissue
    before purchasing it ?

    Read More
    Answer

    Thank you for contacting us. Because we carry over 80,000 products, it isn't feasible for us to keep small sample sizes of our products.

    We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase.

    Asthe product is to be used in an untested species or application, youare eligible for our testing discount program though:

    To our knowledge, ab53233 has not been tested in zebrafish yet. However, the immunogen reveals a sequencesimilarity of 73% and therefore this antibody might cross-react.Thus, I can offer a discount off a future purchase if you buy ab53233 now, test it in zebrafish and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody.

    If you are interested in this offer, please follow these steps:

    1. Reply to this e-mail to let me know that you would like to proceed and test ab53233 in zebrafish. I will then send a discount code. This code must be issued before purchasing ab53233 so please wait for my reply before ordering.

    2. Purchase ab53233 either by phone, fax, or online (www.abcam.com).

    3. Test it in zebrafish.

    4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out more about our Abreview system, please visit: https://www.abcam.com/abreviews.

    5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue.

    We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab53233 turns out to be unsuitable for zebrafish, you will still receive the discount on your next purchase after your Abreview has been submitted.

    Also, please find on our website (https://www.abcam.com/zebrafish) and attached a list of all our antibodies which we guarantee to work with zebrafish.

    Please let me know if you have any questions about this offer and I would be happy to help you further.

    The Terms and Conditions of this offer can be found at: https://www.abcam.com/collaborationdiscount

    Read More

    Question

    Dir Sir or Madame,   I was looking for the amino acid sequence of the epitope your otoferlin antibody (ab53233) is directed against and so far I were not successful on your homepage or in the data sheet. We use your ab a lot and for an urgent experiment, we would need this information. Thank you very much for your help.   Kind regards,     Dr. Christoph Franz Tübingen Hearing Research Centre -THRC ENT University Clinic  Molecular Physiology of Hearing AG Prof. Dr. M. Knipper Elfriede-Aulhorn-Str. 5 72076 Tübingen Germany c.franz@uni-tuebingen.de Tel.:  +49 (0)7071 29 88246 Fax.: +49 (0)707129 4950  

    Read More
    Answer

    Thank you for your inquiry. The immunogen this antibody was raised against is a tagged fusion protein, corresponding to amino acids 1-395 of Human Otoferlin. (Immunogen section on the datasheet). We are not aware that the epitope that is recognized by this antibody was specifically mapped or further narrowed down. I am sorry that I could not provide you with the requested information on this occasion.  

    Read More

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