重组Anti-Osteoprotegerin抗体[EPR3592] (ab124820)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Osteoprotegerin antibody [EPR3592] (ab124820)](https://www.abcam.cn/ps/products/124/ab124820/Images/ab124820-138743-anti-osteoprotegerin-antibody-epr3592-immunohistochemistry.gif "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Osteoprotegerin antibody [EPR3592] (ab124820)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3592] to Osteoprotegerin
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Osteoprotegerin抗体[EPR3592]
参阅全部 Osteoprotegerin 一抗 -
描述
兔单克隆抗体[EPR3592] to Osteoprotegerin -
宿主
Rabbit -
经测试应用
适用于: IHC-P, ICC/IFmore details
不适用于: Flow Cyt,IP or WB -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human Osteoprotegerin aa 50-150. The exact sequence is proprietary.
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阳性对照
- Fetal kidney lysate. Human kidney tissue. Human heart tissue. HeLa cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR3592 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab124820于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | (1) |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
| ICC/IF |
1/100 - 1/250.
|
| 说明 |
|---|
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
|
ICC/IF
1/100 - 1/250. |
应用说明
Is unsuitable for Flow Cyt,IP or WB.
靶标
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功能
Acts as decoy receptor for RANKL and thereby neutralizes its function in osteoclastogenesis. Inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro. Bone homeostasis seems to depend on the local RANKL/OPG ratio. May also play a role in preventing arterial calcification. May act as decoy receptor for TRAIL and protect against apoptosis. TRAIL binding blocks the inhibition of osteoclastogenesis. -
组织特异性
Highly expressed in adult lung, heart, kidney, liver, spleen, thymus, prostate, ovary, small intestine, thyroid, lymph node, trachea, adrenal gland, testis, and bone marrow. Detected at very low levels in brain, placenta and skeletal muscle. Highly expressed in fetal kidney, liver and lung. -
疾病相关
Defects in TNFRSF11B are the cause of juvenile Paget disease (JPD) [MIM:239000]; also known as hyperostosis corticalis deformans juvenilis or hereditary hyperphosphatasia or chronic congenital idiopathic hyperphosphatasia. JPD is a rare autosomal recessive osteopathy that presents in infancy or early childhood. The disorder is characterized by rapidly remodeling woven bone, osteopenia, debilitating fractures, and deformities due to a markedly accelerated rate of bone remodeling throughout the skeleton. Approximately 40 cases of JPD have been reported worldwide. Unless it is treated with drugs that block osteoclast-mediated skeletal resorption, the disease can be fatal. -
序列相似性
Contains 2 death domains.
Contains 4 TNFR-Cys repeats. -
翻译后修饰
N-glycosylated. Contains sialic acid residues.
The N-terminus is blocked. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 4982 Human
- Omim: 602643 Human
- SwissProt: O00300 Human
- Unigene: 81791 Human
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别名
- MGC29565 antibody
- OCIF antibody
- OPG antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Osteoprotegerin antibody [EPR3592] (ab124820)
ab124820 at 1/100 dilution, staining TNFRSF11B in Formalin fixed Paraffin-embedded Human kidney tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Osteoprotegerin antibody [EPR3592] (ab124820)](https://www.abcam.cn/ps/products/124/ab124820/Images/ab124820-138741-anti-osteoprotegerin-antibody-epr3592-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Osteoprotegerin antibody [EPR3592] (ab124820)ab124820 at 1/100 dilution, staining TNFRSF11B in Formalin fixed Paraffin-embedded Human heart tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-Osteoprotegerin antibody [EPR3592] (ab124820)](https://www.abcam.cn/ps/products/124/ab124820/Images/ab124820-138740-anti-osteoprotegerin-antibody-epr3592-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Osteoprotegerin antibody [EPR3592] (ab124820)ab124820, at 1/100 dilution, staining TNFRSF11B in HeLa cells. -
![Anti-Osteoprotegerin antibody [EPR3592] (ab124820)](https://www.abcam.cn/ps/products/124/ab124820/Images/ab124820-6-benefits-of-recombinant-antibodies.png)
Anti-Osteoprotegerin antibody [EPR3592] (ab124820)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab124820 被引用在 4 文献中.
- Lv XF et al. Nano Sized Hydroxyapatite-Polylactic Acid-Vancomycin in Alleviation of Chronic Osteomyelitis. Drug Des Devel Ther 16:1983-1993 (2022). PubMed: 35783197
- Deng B et al. Exendin-4 promotes osteogenic differentiation of adipose-derived stem cells and facilitates bone repair. Mol Med Rep 20:4933-4942 (2019). PubMed: 31661134
- De Toni EN et al. Correlation Between Baseline Osteoprotegerin Serum Levels and Prognosis of Advanced-Stage Colorectal Cancer Patients. Cell Physiol Biochem 45:605-613 (2018). PubMed: 29402836
- Ramos YF et al. A gain of function mutation in TNFRSF11B encoding osteoprotegerin causes osteoarthritis with chondrocalcinosis. Ann Rheum Dis N/A:N/A (2014). PubMed: 24743232