Anti-Osteoprotegerin抗体(ab9986)
Key features and details
- Rabbit polyclonal to Osteoprotegerin
- Suitable for: WB
- Reacts with: Human
- Isotype: unknown
概述
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产品名称
Anti-Osteoprotegerin抗体
参阅全部 Osteoprotegerin 一抗 -
描述
兔多克隆抗体to Osteoprotegerin -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment corresponding to Human Osteoprotegerin.
Database link: O00300 -
常规说明
This product is no longer batch tested in IHC, for an IHC validated antibody please see ab124820
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Lyophilized:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary. -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
No preservative, sterile filtered -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
unknown -
轻链类型
unknown -
研究领域
相关产品
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Compatible Secondaries
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 46 kDa).
To detect hOPG by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hOPG is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
| 说明 |
|---|
|
WB
Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 46 kDa). To detect hOPG by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hOPG is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
靶标
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功能
Acts as decoy receptor for RANKL and thereby neutralizes its function in osteoclastogenesis. Inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro. Bone homeostasis seems to depend on the local RANKL/OPG ratio. May also play a role in preventing arterial calcification. May act as decoy receptor for TRAIL and protect against apoptosis. TRAIL binding blocks the inhibition of osteoclastogenesis. -
组织特异性
Highly expressed in adult lung, heart, kidney, liver, spleen, thymus, prostate, ovary, small intestine, thyroid, lymph node, trachea, adrenal gland, testis, and bone marrow. Detected at very low levels in brain, placenta and skeletal muscle. Highly expressed in fetal kidney, liver and lung. -
疾病相关
Defects in TNFRSF11B are the cause of juvenile Paget disease (JPD) [MIM:239000]; also known as hyperostosis corticalis deformans juvenilis or hereditary hyperphosphatasia or chronic congenital idiopathic hyperphosphatasia. JPD is a rare autosomal recessive osteopathy that presents in infancy or early childhood. The disorder is characterized by rapidly remodeling woven bone, osteopenia, debilitating fractures, and deformities due to a markedly accelerated rate of bone remodeling throughout the skeleton. Approximately 40 cases of JPD have been reported worldwide. Unless it is treated with drugs that block osteoclast-mediated skeletal resorption, the disease can be fatal. -
序列相似性
Contains 2 death domains.
Contains 4 TNFR-Cys repeats. -
翻译后修饰
N-glycosylated. Contains sialic acid residues.
The N-terminus is blocked. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 4982 Human
- Omim: 602643 Human
- SwissProt: O00300 Human
- Unigene: 81791 Human
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别名
- MGC29565 antibody
- OCIF antibody
- OPG antibody
see all
图片
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Western blot - Anti-Osteoprotegerin antibody (ab9986)All lanes : Anti-Osteoprotegerin antibody (ab9986) at 1 µg/ml
Lane 1 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 46 kDa
Observed band size: 56 kDa why is the actual band size different from the predicted?
Additional bands at: 32 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 150 seconds
Osteoprotegerin contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
实验方案
数据表及文件
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Datasheet download
文献 (21)
ab9986 被引用在 21 文献中.
- Li Y et al. Melatonin promotes the restoration of bone defects via enhancement of miR-335-5p combined with inhibition of TNFα/NF-κB signaling. FASEB J 37:e22711 (2023). PubMed: 36520091
- Qin H & Cai J Effect of periostin on bone metabolic and autophagy factors during tooth eruption in mice. Open Life Sci 18:20220663 (2023). PubMed: 37589010
- Xu Y et al. Expression of spleen macrophages in a mouse model of alveolar bone resorption periodontitis. Cell Mol Biol (Noisy-le-grand) 69:125-131 (2023). PubMed: 37605579
- Lima IFP et al. RANK, RANKL, and OPG in Dentigerous Cyst, Odontogenic Keratocyst, and Ameloblastoma: A Meta-Analysis. Braz Dent J 32:16-25 (2021). PubMed: 33913997
- Zhang JR et al. TNFRSF11B Suppresses Memory CD4+ T Cell Infiltration in the Colon Cancer Microenvironment: A Multiomics Integrative Analysis. Front Immunol 12:742358 (2021). PubMed: 34938284
