Anti-Osteopontin抗体(ab8448)
Key features and details
- Rabbit polyclonal to Osteopontin
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Osteopontin抗体
参阅全部 Osteopontin 一抗 -
描述
兔多克隆抗体to Osteopontin -
宿主
Rabbit -
特异性
Osteopontin is directed against human osteopontin. The antibody recognizes the full-length osteopontin protein (which runs at 66 kDa on westerns), as well as the C-terminal fragments of both thrombin and MMP-cleaved OPN. The 32 kDa MMP-cleaved C-fragment is recognized, but not the 40 kDa N-terminal fragment. Reactivity is reported to occur with osteopontin from swine, dog, mouse and rat.
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经测试应用
适用于: IHC-Pmore details
不适用于: ICC/IF or WB -
种属反应性
与反应: Human -
免疫原
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Preservative: 0.1% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Immunohistochemistry kits
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Isotype control
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Related Products
应用
靶标
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功能
Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.
Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity. -
组织特异性
Bone. Found in plasma. -
序列相似性
Belongs to the osteopontin family. -
翻译后修饰
Extensively phosphorylated on clustered serine residues.
N- and O-glycosylated.
Phosphorylation sites are present in the extracelllular medium. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 6696 Human
- Omim: 166490 Human
- SwissProt: P10451 Human
- Unigene: 313 Human
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别名
- BNSP antibody
- Bone sialoprotein 1 antibody
- Bone sialoprotein I antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Osteopontin antibody (ab8448)
Human Breast tumour section. Osteopontin is a normal component of elastic fibers in the breast (shown heavily stained in this section). There is also weak staining of the extracellular matrix. Osteopontin is not believed to be expressed inside breast tumour cells, and there is no staining in the intracellular region of the breast cells in this section.
Osteopontin antibody (ab8448) used at 1:100-1:300. No antigen retrieval is required.
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Schematic Diagram - Anti-Osteopontin antibody (ab8448)This image is courtesy of Agnihotri et al, JBC 2001OPN is cleaved by MMP to yield 2 fragments, which migrate at 40kD(N terminal) and 32kD (C terminal). The C terminal fragment can undergo further cleavage by both of these MMPs (see Agnihotri et al, JBC 2001 for further details). The epitope recognised by ab8448 is shown in violet. This antibody detects the full length OPN and the 32kD fragment. It does not recognise the 40kD fragment.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (444)
ab8448 被引用在 444 文献中.
- Lu Y et al. Impairment of Autophagy Mediates the Uric-Acid-Induced Phenotypic Transformation of Vascular Smooth Muscle Cells. Pharmacology 109:34-42 (2024). PubMed: 38011839
- Kim K et al. Cell Competition Shapes Metastatic Latency and Relapse. Cancer Discov 13:85-97 (2023). PubMed: 36098678
- Abramov A et al. Bioprosthetic heart valve structural degeneration associated with metabolic syndrome: Mitigation with polyoxazoline modification. Proc Natl Acad Sci U S A 120:e2219054120 (2023). PubMed: 36574676
- Bai Q et al. Microglia-Derived Spp1 Promotes Pathological Retinal Neovascularization via Activating Endothelial Kit/Akt/mTOR Signaling. J Pers Med 13:N/A (2023). PubMed: 36675807
- Xie W et al. Herpud1 deficiency alleviates homocysteine-induced aortic valve calcification. Cell Biol Toxicol 39:2665-2684 (2023). PubMed: 36746840