Anti-Osteopontin抗体(ab11503)
Key features and details
- Goat polyclonal to Osteopontin
- Suitable for: Indirect ELISA, ICC/IF, Blocking, IHC-Fr, WB, Neutralising
- Reacts with: Mouse, Rat
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Osteopontin抗体
参阅全部 Osteopontin 一抗 -
描述
山羊多克隆抗体to Osteopontin -
宿主
Goat -
经测试应用
适用于: Indirect ELISA, ICC/IF, Blocking, IHC-Fr, WB, Neutralisingmore details -
种属反应性
与反应: Mouse, Rat -
免疫原
Recombinant full length protein (Mouse) expressed in NSO cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Constituents: PBS, 5% Trehalose -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab11503于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Indirect ELISA |
Use at an assay dependent concentration.
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| ICC/IF | (1) |
Use at an assay dependent concentration.
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| Blocking |
Use at an assay dependent concentration.
Blocks osteopontin in smooth muscle cell samples. |
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| IHC-Fr |
Use at an assay dependent concentration. PubMed: 17224478
|
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| WB |
Use at an assay dependent concentration.
|
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| Neutralising |
Use at an assay dependent concentration.
The Neutralization Dose50 (ND50) for anti-mouse osteopontin is approximately 1 to 3 µg/ml in the presence of 2 µg/ml of recombinant mouse osteopontin using 293 cells. |
| 说明 |
|---|
|
Indirect ELISA
Use at an assay dependent concentration. |
|
ICC/IF
Use at an assay dependent concentration. |
|
Blocking
Use at an assay dependent concentration. Blocks osteopontin in smooth muscle cell samples. |
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IHC-Fr
Use at an assay dependent concentration. PubMed: 17224478 |
|
WB
Use at an assay dependent concentration. |
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Neutralising
Use at an assay dependent concentration. The Neutralization Dose50 (ND50) for anti-mouse osteopontin is approximately 1 to 3 µg/ml in the presence of 2 µg/ml of recombinant mouse osteopontin using 293 cells. |
靶标
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功能
Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.
Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity. -
组织特异性
Bone. Found in plasma. -
序列相似性
Belongs to the osteopontin family. -
翻译后修饰
Extensively phosphorylated on clustered serine residues.
N- and O-glycosylated.
Phosphorylation sites are present in the extracelllular medium. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 20750 Mouse
- Entrez Gene: 25353 Rat
- SwissProt: P10923 Mouse
- SwissProt: P08721 Rat
- Unigene: 288474 Mouse
- Unigene: 8871 Rat
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别名
- BNSP antibody
- Bone sialoprotein 1 antibody
- Bone sialoprotein I antibody
see all
实验方案
数据表及文件
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Datasheet download
文献 (16)
ab11503 被引用在 16 文献中.
- Zhang XZ et al. Application of metabolomics in urolithiasis: the discovery and usage of succinate. Signal Transduct Target Ther 8:41 (2023). PubMed: 36681678
- Gai C et al. Mechanistic Insights into the Role of OPN in Mediating Brain Damage via Triggering Lysosomal Damage in Microglia/Macrophage. Cells 12:N/A (2023). PubMed: 36980197
- Guo R et al. Biomimetic composite hydrogel promotes new bone formation in rat bone defects through regulation of miR-19b-3p/WWP1 axis by loaded extracellular vesicles. J Nanobiotechnology 21:459 (2023). PubMed: 38037135
- Zhang S et al. Induction of ferroptosis promotes vascular smooth muscle cell phenotypic switching and aggravates neointimal hyperplasia in mice. Mol Med 28:121 (2022). PubMed: 36192693
- Liao H et al. Osteopontin-integrin signaling positively regulates neuroplasticity through enhancing neural autophagy in the peri-infarct area after ischemic stroke. Am J Transl Res 14:7726-7743 (2022). PubMed: 36505285
