重组Anti-Osteocalcin抗体[EPR3690] (ab133612)
![Western blot - Anti-Osteocalcin antibody [EPR3690] (ab133612)](https://www.abcam.cn/ps/products/133/ab133612/Images/ab133612-314274-anti-osteocalcin-antibody-epr3690-western-blot.jpg "Western blot - Anti-Osteocalcin antibody [EPR3690] (ab133612)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3690] to Osteocalcin
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
概述
-
产品名称
Anti-Osteocalcin抗体[EPR3690]
参阅全部 Osteocalcin 一抗 -
描述
兔单克隆抗体[EPR3690] to Osteocalcin -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human Osteocalcin aa 50-150. The exact sequence is proprietary.
Database link: P02818 -
阳性对照
- WB: Human Osteocalcin recombinant protein, Human articular cartilage tissue lysate
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR3690 -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133612于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 11 kDa.
|
| 说明 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 11 kDa. |
靶标
-
功能
Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium. -
序列相似性
Belongs to the osteocalcin/matrix Gla protein family.
Contains 1 Gla (gamma-carboxy-glutamate) domain. -
翻译后修饰
Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium. -
细胞定位
Secreted. - Information by UniProt
-
数据库链接
- Entrez Gene: 632 Human
- Omim: 112260 Human
- SwissProt: P02818 Human
- Unigene: 654541 Human
-
别名
- BGLAP antibody
- BGP antibody
- Bone gamma carboxyglutamate (gla) protein antibody
see all
图片
-
Western blot - Anti-Osteocalcin antibody [EPR3690] (ab133612)Anti-Osteocalcin antibody [EPR3690] (ab133612) at 1.2 µg/ml (purified) + Human osteocalcin recombinant protein at 0.015 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 11 kDaBlocking and diluting buffer: 5% NFDM/TBST.
-
![Western blot - Anti-Osteocalcin antibody [EPR3690] (ab133612)](https://www.abcam.cn/ps/products/133/ab133612/Images/ab133612-506191-anti-osteocalcin-antibody-epr3690-western-blot-cartilage-tissue-lysate-human.jpg)
Western blot - Anti-Osteocalcin antibody [EPR3690] (ab133612)Anti-Osteocalcin antibody [EPR3690] (ab133612) at 1/1000 dilution + Human articular cartilage tissue lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 11 kDa
Observed band size: 19 kDa why is the actual band size different from the predicted?
Exposure time: 158 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.
-
![Western blot - Anti-Osteocalcin antibody [EPR3690] (ab133612)](https://www.abcam.cn/ps/products/133/ab133612/Images/ab133612-147467-anti-osteocalcin-antibody-epr3690-western-blot.jpg)
Western blot - Anti-Osteocalcin antibody [EPR3690] (ab133612)Anti-Osteocalcin antibody [EPR3690] (ab133612) at 1/1000 dilution (Unpurified) + Human Osteocalcin recombinant protein at 0.01 µg
Predicted band size: 11 kDa -
![Anti-Osteocalcin antibody [EPR3690] (ab133612)](https://www.abcam.cn/ps/products/133/ab133612/Images/ab133612-3-benefits-of-recombinant-antibodies.png)
Anti-Osteocalcin antibody [EPR3690] (ab133612)
数据表及文件
-
SDS download
-
Datasheet download
文献 (71)
ab133612 被引用在 71 文献中.
- Lin H et al. Low-intensity pulsed ultrasound enhances immunomodulation and facilitates osteogenesis of human periodontal ligament stem cells by inhibiting the NF-κB pathway. Cell Tissue Bank 24:45-58 (2023). PubMed: 35644018
- Yu L et al. Carboxymethyl chitosan-alginate enhances bone repair effects of magnesium phosphate bone cement by activating the FAK-Wnt pathway. Bioact Mater 20:598-609 (2023). PubMed: 35846837
- Sun B et al. Irisin reduces bone fracture by facilitating osteogenesis and antagonizing TGF-β/Smad signaling in a growing mouse model of osteogenesis imperfecta. J Orthop Translat 38:175-189 (2023). PubMed: 36439629
- Ye Y et al. Integrative Analysis of ceRNA Networks in human periodontal ligament stem cells under hypoxia. Oral Dis 29:1197-1213 (2023). PubMed: 34874587
- Zhang D et al. Casticin promotes osteogenic differentiation of bone marrow stromal cells and improves osteoporosis in rats by regulating nuclear factor-κB/mitogen-activated protein kinase. Int J Rheum Dis 26:80-87 (2023). PubMed: 36195975