Anti-OSBPL5/ORP5抗体(ab59016)
Key features and details
- Goat polyclonal to OSBPL5/ORP5
- Suitable for: IHC-P, WB, ICC/IF, Flow Cyt
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-OSBPL5/ORP5抗体
参阅全部 OSBPL5/ORP5 一抗 -
描述
山羊多克隆抗体to OSBPL5/ORP5 -
宿主
Goat -
特异性
This antibody is expected to recognise both reported isoforms (NP_065947.1; NP_663613.1). -
经测试应用
适用于: IHC-P, WB, ICC/IF, Flow Cytmore details -
种属反应性
与反应: Human
预测可用于: Mouse
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Jurkat and rat spleen cell lysates. ICC/IF: HeLa and U2OS cells. IHC-P: Human kidney and liver tissues. Flow-Cyt: HeLa cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab59016于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB |
Use a concentration of 0.03 - 0.3 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 99 kDa).
1 hour primary incubation is recommended for this product. |
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| ICC/IF | (1) |
Use a concentration of 10 µg/ml.
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| Flow Cyt |
Use a concentration of 10 µg/ml.
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| 说明 |
|---|
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IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
WB
Use a concentration of 0.03 - 0.3 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 99 kDa). 1 hour primary incubation is recommended for this product. |
|
ICC/IF
Use a concentration of 10 µg/ml. |
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Flow Cyt
Use a concentration of 10 µg/ml. |
靶标
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功能
Lipid transporter involved in lipid countertransport between the endoplasmic reticulum and the plasma membrane: specifically exchanges phosphatidylserine with phosphatidylinositol 4-phosphate (PI4P), delivering phosphatidylserine to the plasma membrane in exchange for PI4P, which is degraded by the SAC1/SACM1L phosphatase in the endoplasmic reticulum. Binds phosphatidylserine and PI4P in a mutually exclusive manner (PubMed:23934110, PubMed:26206935). May cooperate with NPC1 to mediate the exit of cholesterol from endosomes/lysosomes (PubMed:21220512). Binds 25-hydroxycholesterol and cholesterol (PubMed:17428193). -
组织特异性
Ubiquitously expressed. -
序列相似性
Belongs to the OSBP family.
Contains 1 PH domain. -
细胞定位
Endoplasmic reticulum membrane. Localizes to the cortical endoplasmic reticulum at the endoplasmic reticulum-plasma membrane contact sites. - Information by UniProt
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数据库链接
- Entrez Gene: 114879 Human
- Entrez Gene: 79196 Mouse
- Omim: 606733 Human
- SwissProt: Q9H0X9 Human
- SwissProt: Q9ER64 Mouse
- Unigene: 436166 Human
- Unigene: 21199 Mouse
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别名
- DKFZp586H1719 antibody
- FLJ31948 antibody
- KIAA1534 antibody
see all
图片
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Western blot - Anti-OSBPL5/ORP5 antibody (ab59016)Anti-OSBPL5/ORP5 antibody (ab59016) at 0.3 µg/ml + Rat Spleen lysate in RIPA buffer at 35 µg
Predicted band size: 99 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?Detected by chemiluminescence.
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Immunocytochemistry/ Immunofluorescence - Anti-OSBPL5/ORP5 antibody (ab59016)ab59016 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OSBPL5/ORP5 antibody (ab59016)ab59016 (5µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Flow Cytometry - Anti-OSBPL5/ORP5 antibody (ab59016)ab59016 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Immunocytochemistry/ Immunofluorescence - Anti-OSBPL5/ORP5 antibody (ab59016)ab59016 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OSBPL5/ORP5 antibody (ab59016)ab59016 (5µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Western blot - Anti-OSBPL5/ORP5 antibody (ab59016)Anti-OSBPL5/ORP5 antibody (ab59016) at 0.1 µg/ml + Jurkat cell lysate in RIPA buffer at 35 µg
Predicted band size: 99 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?Detected by chemiluminescence.
数据表及文件
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Datasheet download
文献 (6)
ab59016 被引用在 6 文献中.
- Hu R et al. The LMCD1-AS1/miR-526b-3p/OSBPL5 axis promotes cell proliferation, migration and invasion in non-small cell lung cancer. BMC Pulm Med 22:30 (2022). PubMed: 35000595
- Song L et al. ORP5 promotes tumor metastasis via stabilizing c-Met in renal cell carcinoma. Cell Death Discov 8:219 (2022). PubMed: 35449154
- Xu M et al. OSBP-Related Protein 5L Maintains Intracellular IP3/Ca2+ Signaling and Proliferation in T Cells by Facilitating PIP2 Hydrolysis. J Immunol 204:1134-1145 (2020). PubMed: 31953353
- Sohn M et al. PI(4,5)P2 controls plasma membrane PI4P and PS levels via ORP5/8 recruitment to ER-PM contact sites. J Cell Biol 217:1797-1813 (2018). PubMed: 29472386
- Du X et al. Oxysterol-binding protein-related protein 5 (ORP5) promotes cell proliferation by activation of mTORC1 signaling. J Biol Chem 293:3806-3818 (2018). PubMed: 29358326
- Ghai R et al. ORP5 and ORP8 bind phosphatidylinositol-4, 5-biphosphate (PtdIns(4,5)P2) and regulate its level at the plasma membrane. Nat Commun 8:757 (2017). PubMed: 28970484
