Anti-OSBPL5/ORP5抗体(ab127171)
Key features and details
- Rabbit polyclonal to OSBPL5/ORP5
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-OSBPL5/ORP5抗体
参阅全部 OSBPL5/ORP5 一抗 -
描述
兔多克隆抗体to OSBPL5/ORP5 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment corresponding to Human OSBPL5/ORP5 aa 242-458 (internal sequence).
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阳性对照
- HeLa and A549 whole cell lysate Breast cancer tissue
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab127171于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
1/100 - 1/1000.
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| WB |
1/500 - 1/3000. Predicted molecular weight: 99 kDa.
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| IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively Tris-EDTA buffer ( pH8.0) may be used.
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| 说明 |
|---|
|
ICC/IF
1/100 - 1/1000. |
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WB
1/500 - 1/3000. Predicted molecular weight: 99 kDa. |
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IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively Tris-EDTA buffer ( pH8.0) may be used. |
靶标
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功能
Lipid transporter involved in lipid countertransport between the endoplasmic reticulum and the plasma membrane: specifically exchanges phosphatidylserine with phosphatidylinositol 4-phosphate (PI4P), delivering phosphatidylserine to the plasma membrane in exchange for PI4P, which is degraded by the SAC1/SACM1L phosphatase in the endoplasmic reticulum. Binds phosphatidylserine and PI4P in a mutually exclusive manner (PubMed:23934110, PubMed:26206935). May cooperate with NPC1 to mediate the exit of cholesterol from endosomes/lysosomes (PubMed:21220512). Binds 25-hydroxycholesterol and cholesterol (PubMed:17428193). -
组织特异性
Ubiquitously expressed. -
序列相似性
Belongs to the OSBP family.
Contains 1 PH domain. -
细胞定位
Endoplasmic reticulum membrane. Localizes to the cortical endoplasmic reticulum at the endoplasmic reticulum-plasma membrane contact sites. - Information by UniProt
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数据库链接
- Entrez Gene: 114879 Human
- Omim: 606733 Human
- SwissProt: Q9H0X9 Human
- Unigene: 436166 Human
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别名
- DKFZp586H1719 antibody
- FLJ31948 antibody
- KIAA1534 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OSBPL5/ORP5 antibody (ab127171)
ab127171, at 1/500 dilution, staining OSBPL5/ORP5 in paraffin-embedded breast cancer tissue by immunohistochemistry
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Immunocytochemistry/ Immunofluorescence - Anti-OSBPL5/ORP5 antibody (ab127171)Paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling OSBPL5/ORP5 using ab127171 at 1/500 dilution (green) in ICC/IF.
The nuclear counter stain is Hoechst 33342 (blue).
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Western blot - Anti-OSBPL5/ORP5 antibody (ab127171)Anti-OSBPL5/ORP5 antibody (ab127171) at 1/500 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 99 kDa
7.5% SDS PAGE
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab127171 被引用在 1 文献中.
- Li J et al. Oxysterol binding protein-related protein 8 mediates the cytotoxicity of 25-hydroxycholesterol. J Lipid Res 57:1845-1853 (2016). PubMed: 27530118