Anti-OS9抗体(ab225915)
Key features and details
- Rabbit polyclonal to OS9
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-OS9抗体
参阅全部 OS9 一抗 -
描述
兔多克隆抗体to OS9 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment corresponding to Human OS9 aa 232-413.
Database link: Q13438 -
阳性对照
- IHC-P: Human testis tissue and lung cancer tissues. WB: HepG2 and HEK-293 whole cell lysates. ICC/IF: HeLa cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
纯度
Protein G purified -
纯化说明
Purity >95%. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab225915于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/20 - 1/200.
|
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| WB |
1/500 - 1/5000. Detects a band of approximately 76 kDa (predicted molecular weight: 76 kDa).
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| ICC/IF |
1/50 - 1/200.
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| 说明 |
|---|
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IHC-P
1/20 - 1/200. |
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WB
1/500 - 1/5000. Detects a band of approximately 76 kDa (predicted molecular weight: 76 kDa). |
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ICC/IF
1/50 - 1/200. |
靶标
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功能
Lectin which functions in endoplasmic reticulum (ER) quality control and ER-associated degradation (ERAD). May bind terminally misfolded non-glycosylated proteins as well as improperly folded glycoproteins, retain them in the ER, and possibly transfer them to the ubiquitination machinery and promote their degradation. Possible targets include TRPV4. -
组织特异性
Ubiquitously expressed. Found as well in all tumor cell lines analyzed, amplified in sarcomas. Highly expressed in osteosarcoma OSA-CL and rhabdomyosarcoma RH30 cell lines. Isoform 2 is the major isoform detected in all cell types examined. -
序列相似性
Belongs to the OS-9 family.
Contains 1 PRKCSH domain. -
翻译后修饰
Intramolecular disulfide bonds.
Isoform 1 and isoform 2 are N-glycosylated. -
细胞定位
Endoplasmic reticulum lumen. - Information by UniProt
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数据库链接
- Entrez Gene: 10956 Human
- Omim: 609677 Human
- SwissProt: Q13438 Human
- Unigene: 527861 Human
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别名
- Amplified in osteosarcoma 9 antibody
- amplified in osteosarcoma antibody
- ERLEC2 antibody
see all
图片
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Western blot - Anti-OS9 antibody (ab225915)All lanes : Anti-OS9 antibody (ab225915) at 1/500 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 76 kDa
Observed band size: 76 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-OS9 antibody (ab225915)HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for OS9 (green) using ab225915 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OS9 antibody (ab225915)Paraffin-embedded human testis tissue stained for OS9 using ab225915 at 1/100 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OS9 antibody (ab225915)Paraffin-embedded human lung cancer tissue stained for OS9 using ab225915 at 1/100 dilution in immunohistochemical analysis.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab225915 尚未被引用在任何文献中。