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Anti-ORP8抗体[mAbcam 60110] (ab60110)

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Western blot - Anti-ORP8 antibody [mAbcam 60110] (ab60110)
  • Western blot - Anti-ORP8 antibody [mAbcam 60110] (ab60110)
  • Flow Cytometry (Intracellular) - Anti-ORP8 antibody [mAbcam 60110] (ab60110)

Key features and details

  • Mouse monoclonal [mAbcam 60110] to ORP8
  • Suitable for: Flow Cyt (Intra), WB
  • Reacts with: Human
  • Isotype: IgG1

概述

  • 产品名称

    Anti-ORP8抗体[mAbcam 60110]
    参阅全部 ORP8 一抗

  • 描述

    小鼠单克隆抗体[mAbcam 60110] to ORP8

  • 宿主

    Mouse

  • 经测试应用

    适用于: Flow Cyt (Intra), WBmore details

  • 种属反应性

    与反应: Human
    预测可用于: Mouse, Horse, Dog, Chimpanzee

  • 免疫原

    Synthetic peptide corresponding to Human ORP8 aa 1-100 (N terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab75267)

  • 阳性对照

    • This antibody gave a positive signal in human kidney tissue lysate and in the following whole cell lysates: HEK293; THP1.

  • 常规说明

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab60110于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (Intra)
Use 1-2µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB (1)
Use a concentration of 5 µg/ml. Detects a band of approximately 101 kDa (predicted molecular weight: 101 kDa).

We recommend using 3% milk as the blocking agent in Western Blot.
说明
Flow Cyt (Intra)
Use 1-2µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB
Use a concentration of 5 µg/ml. Detects a band of approximately 101 kDa (predicted molecular weight: 101 kDa).

We recommend using 3% milk as the blocking agent in Western Blot.

靶标

  • 相关性

    ORP8 is a member of the oxysterol-binding protein related protein family. In humans, the gene family consists of 12 members, and extensive splice variation increases the number of encoded protein products substantially. The ORPs have been implicated as sterol sensors that regulate a number of cellular functions including sterol and neutral lipid metabolism, intracellular lipid transport, membrane trafficking and cell signaling. ORP8 has been recently shown to act as a sterol sensor that affects the reverse cholesterol transport process via modulation of ABCA1 expression and macrophage cholesterol efflux.

  • 细胞定位

    Endoplasmic reticulum

  • 数据库链接

    • 别名

      • DKFZp686A11164 antibody
      • KIAA1451 antibody
      • MGC126578 antibody
      • MGC133203 antibody
      • MST120 antibody
      • MSTP120 antibody
      • ORP 8 antibody
      • OSBP related protein 8 antibody
      • OSBP10 antibody
      • OSBPL8 antibody
      • Oxysterol binding protein like 8 antibody
      • Oxysterol binding protein like protein 8 antibody
      • Oxysterol binding protein related protein 8 antibody
      see all

    图片

    • Western blot - Anti-ORP8 antibody [mAbcam 60110] (ab60110)
      Western blot - Anti-ORP8 antibody [mAbcam 60110] (ab60110)
      All lanes : Anti-ORP8 antibody [mAbcam 60110] (ab60110) at 5 µg/ml

      Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
      Lane 2 : THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate
      Lane 3 : Human kidney tissue lysate - total protein (ab30203)

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/10000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 101 kDa
      Observed band size: 101 kDa


      Exposure time: 8 minutes


      We recommend using 3% milk as the blocking agent in Western Blot.

    • Western blot - Anti-ORP8 antibody [mAbcam 60110] (ab60110)
      Western blot - Anti-ORP8 antibody [mAbcam 60110] (ab60110)
      All lanes : Anti-ORP8 antibody [mAbcam 60110] (ab60110) at 5 µg/ml

      Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
      Lane 2 : THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate
      Lane 3 : Human kidney tissue lysate - total protein (ab30203)

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 101 kDa
      Observed band size: 101 kDa
      Additional bands at: 44 kDa, 55 kDa, 80 kDa, 85 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 30 seconds


      1% BSA was used as a blocking agent.

    • Flow Cytometry (Intracellular) - Anti-ORP8 antibody [mAbcam 60110] (ab60110)
      Flow Cytometry (Intracellular) - Anti-ORP8 antibody [mAbcam 60110] (ab60110)
      Overlay histogram showing HEK293 cells stained with ab60110 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab60110, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    文献 (0)

    发表研究结果有使用 ab60110？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab60110 尚未被引用在任何文献中。

    客户评价及客户问答

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    Western blot abreview for Anti-ORP8 antibody [mAbcam 60110]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa, Mel202, A431)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    30 µg
    Specification
    HeLa, Mel202, A431
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jul 22 2016

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