重组Anti-ORP1抗体[EPR8646] (ab131165)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8646] to ORP1
- Suitable for: Flow Cyt (Intra), ICC/IF, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-ORP1抗体[EPR8646]
参阅全部 ORP1 一抗 -
描述
兔单克隆抗体[EPR8646] to ORP1 -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), ICC/IF, WBmore details
不适用于: IP -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: A549, 293T, U87-MG cell lysates; Mouse brain and heart lysates; Rat brain lysates Flow Cyt (intra): A549 cells ICC/Flow Cyt (intra): A549 and U87-MG cells
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR8646 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab131165于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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ICC/IF |
1/200.
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WB |
1/1000 - 1/10000. Detects a band of approximately 50-108 kDa (predicted molecular weight: 108 kDa).
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说明 |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ICC/IF
1/200. |
WB
1/1000 - 1/10000. Detects a band of approximately 50-108 kDa (predicted molecular weight: 108 kDa). |
靶标
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功能
Binds phospholipids; exhibits strong binding to phosphatidic acid and weak binding to phosphatidylinositol 3-phosphate. -
序列相似性
Belongs to the OSBP family.
Contains 3 ANK repeats.
Contains 1 PH domain. - Information by UniProt
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数据库链接
- Entrez Gene: 114876 Human
- Entrez Gene: 64291 Mouse
- Entrez Gene: 259221 Rat
- Omim: 606730 Human
- SwissProt: Q9BXW6 Human
- SwissProt: Q91XL9 Mouse
- SwissProt: Q8K4M9 Rat
- Unigene: 370725 Human
see all -
别名
- FLJ10217 antibody
- ORP 1 antibody
- ORP-1 antibody
see all
图片
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All lanes : Anti-ORP1 antibody [EPR8646] (ab131165) at 1/10000 dilution (Purified)
Lane 1 : A549 (Human lung carcinoma epithelial cell) whole cell lysates
Lane 2 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell) whole cell lysates
Lane 3 : Mouse brain lysates
Lane 4 : Mouse heart lysates
Lane 5 : Rat brain lysates
Lane 6 : Rat heart lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 108 kDa
Observed band size: 108,50 kDa why is the actual band size different from the predicted?Two mRNA variants produce a 108 kDa long ORP1 (ORP1L) and a 50 KDa short ORP1 (ORP1S) separately, which is consistent with what has been described in PMID: 12631712.
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Anti-ORP1 antibody [EPR8646] (ab131165) at 1/1000 dilution + 293T (Human embryonic kidney epithelial cell) whole cell lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 108 kDa
Observed band size: 108,50 kDa why is the actual band size different from the predicted?Two mRNA variants produce a 108 kDa long ORP1 (ORP1L) and a 50 KDa short ORP1 (ORP1S) separately, which is consistent with what has been described in PMID: 12631712.
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Intracellular Flow Cytometry analysis of A549 (Human lung carcinoma epithelial cell) cells labeling ORP1 with purified ab131165 at 1/60 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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Immunocytochemistry/ Immunofluorescence analysis of A549 (Human lung carcinoma epithelial cell) cells labeling ORP1 with purified ab131165 at 1:200 dilution (2.8 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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All lanes : Anti-ORP1 antibody [EPR8646] (ab131165) at 1/1000 dilution (unpurified)
Lane 1 : A549 cell lysate
Lane 2 : 293T cell lysate
Lane 3 : U87-MG cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 108 kDa
Observed band size: 50-108 kDa why is the actual band size different from the predicted? -
ab131165 (unpurified) staining ORP1 in U87-MG (human glioblastoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 100% methanol. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000.
DAPI was used as a nuclear counterstain and the negative control was PBS only.
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ab131165 (unpurified) at 1/10 dilution staining ORP1 in permeabilized A549 cells by intracellular flow cytometry (red). Rabbit IgG negative control (green).
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (11)
ab131165 被引用在 11 文献中.
- Schuler B et al. Host Lipid Transport Protein ORP1 Is Necessary for Coxiella burnetii Growth and Vacuole Expansion in Macrophages. mSphere 8:e0010423 (2023). PubMed: 37017523
- Boutry M & Kim PK ORP1L mediated PI(4)P signaling at ER-lysosome-mitochondrion three-way contact contributes to mitochondrial division. Nat Commun 12:5354 (2021). PubMed: 34504082
- Singhal A et al. 2-Hydroxypropyl-gamma-cyclodextrin overcomes NPC1 deficiency by enhancing lysosome-ER association and autophagy. Sci Rep 10:8663 (2020). PubMed: 32457374
- Zhao K et al. Oxysterol-binding protein-related protein 1 variants have opposing cholesterol transport activities from the endolysosomes. Mol Biol Cell 31:793-802 (2020). PubMed: 32023146
- Dong J et al. Allosteric enhancement of ORP1-mediated cholesterol transport by PI(4,5)P2/PI(3,4)P2. Nat Commun 10:829 (2019). PubMed: 30783101