AB131165

重组Anti-ORP1抗体[EPR8646]

Anti-ORP1 antibody [EPR8646]

RabMAb
Recombinant
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(13 Publications)

Rabbit Recombinant Monoclonal ORP1 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 13 publications.

查看别名

ORP1, OSBP8, OSBPL1, OSBPL1B, OSBPL1A, Oxysterol-binding protein-related protein 1, ORP-1, OSBP-related protein 1

7 Images

Immunocytochemistry/ Immunofluorescence - Anti-ORP1 antibody [EPR8646] (AB131165)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-ORP1 antibody [EPR8646] (AB131165)

ab131165 (unpurified) staining ORP1 in U87-MG (human glioblastoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 100% methanol. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000.

DAPI was used as a nuclear counterstain and the negative control was PBS only.

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ORP1 antibody [EPR8646] (AB131165)

Immunocytochemistry/ Immunofluorescence analysis of A549 (Human lung carcinoma epithelial cell) cells labeling ORP1 with purified ab131165 at 1 : 200 dilution (2.8 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1 : 200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1 : 1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

Flow Cytometry (Intracellular) - Anti-ORP1 antibody [EPR8646] (AB131165)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-ORP1 antibody [EPR8646] (AB131165)

ab131165 (unpurified) at 1/10 dilution staining ORP1 in permeabilized A549 cells by intracellular flow cytometry (red). Rabbit IgG negative control (green).

Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-ORP1 antibody [EPR8646] (AB131165)

Intracellular Flow Cytometry analysis of A549 (Human lung carcinoma epithelial cell) cells labeling ORP1 with purified ab131165 at 1/60 dilution (10 μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

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Western blot - Anti-ORP1 antibody [EPR8646] (AB131165)

All lanes:

Western blot - Anti-ORP1 antibody [EPR8646] (ab131165) at 1/1000 dilution

Lane 1:

A549 cell lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Lane 3:

U87-MG cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 108 kDa

Observed band size: 50-108 kDa

false

Lab

Western blot - Anti-ORP1 antibody [EPR8646] (AB131165)

Two mRNA variants produce a 108 kDa long ORP1 (ORP1L) and a 50 KDa short ORP1 (ORP1S) separately, which is consistent with what has been described in PMID : 12631712.

All lanes:

Western blot - Anti-ORP1 antibody [EPR8646] (ab131165) at 1/1000 dilution

All lanes:

293T (Human embryonic kidney epithelial cell) whole cell lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 108 kDa

Observed band size: 108 kDa,50 kDa

false

Lab

Western blot - Anti-ORP1 antibody [EPR8646] (AB131165)

Two mRNA variants produce a 108 kDa long ORP1 (ORP1L) and a 50 KDa short ORP1 (ORP1S) separately, which is consistent with what has been described in PMID : 12631712.

All lanes:

Western blot - Anti-ORP1 antibody [EPR8646] (ab131165) at 1/10000 dilution

Lane 1:

A549 (Human lung carcinoma epithelial cell) whole cell lysates at 20 µg

Lane 2:

U-87 MG (Human glioblastoma-astrocytoma epithelial cell) whole cell lysates at 20 µg

Lane 3:

Mouse brain lysates at 20 µg

Lane 4:

Mouse heart lysates at 20 µg

Lane 5:

Rat brain lysates at 20 µg

Lane 6:

Rat heart lysates at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 108 kDa

Observed band size: 108 kDa,50 kDa

false

不同偶联物与剂型 (1)

Carrier free

Anti-ORP1 antibody [EPR8646] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR8646

亚型

IgG

不含载体蛋白

反应种属

Mouse, Rat, Human

应用

ICC/IF, WB, Flow Cyt (Intra)

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

ORP1 also known as oxysterol-binding protein-related protein 1 is a member of the OSBP (oxysterol-binding protein) family. It functions as a lipid transfer protein involved in the regulation of intracellular lipid dynamics. ORP1 weighs approximately 108 kDa and is ubiquitously expressed in various tissues with higher levels in the liver and brain. The protein interacts with lipids and plays a role in the control of cholesterol and phosphoinositide levels by transporting them between cellular membranes.

Biological function summary

ORP1 engages in intracellular lipid transport influencing lipid homeostasis and vesicular trafficking. It is part of a protein complex that associates with late endosomes and the endoplasmic reticulum membrane. ORP1 contributes to the regulation of endosomal positioning and transport by modulating the activity of motor proteins on endosomal surfaces. This protein interaction assists in maintaining an appropriate balance of cholesterol and other sterols within cells impacting overall cellular health.

Pathways

Several cellular mechanisms involve ORP1 including the sterol regulatory element-binding protein (SREBP) pathway and the PI3K/Akt signaling pathway. ORP1 interacts with NPC1 a protein critical for cholesterol transport within the SREBP pathway to facilitate the regulation of lipid metabolism. Furthermore ORP1 influences the PI3K/Akt signaling pathway by interacting with other lipid-related proteins coordinating cellular responses to lipid levels and adapting to changes in nutritional states.

Abnormalities related to ORP1 function have connections to neurodegenerative diseases and metabolic syndromes. In neurodegenerative conditions like Niemann-Pick disease ORP1 interacts with the NPC1 and NPC2 proteins both of which are important for normal cholesterol trafficking. In metabolic disorders this protein’s role in lipid regulation links it to obesity and associated metabolic syndrome where dysregulation of lipid storage and transport is common. These connections render ORP1 a potential target for therapeutic intervention in these diseases.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Binds phospholipids; exhibits strong binding to phosphatidic acid and weak binding to phosphatidylinositol 3-phosphate (By similarity). Stabilizes GTP-bound RAB7A on late endosomes/lysosomes and alters functional properties of late endocytic compartments via its interaction with RAB7A (PubMed : 16176980). Binds 25-hydroxycholesterol and cholesterol (PubMed : 17428193).

See full target information OSBPL1A

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文献 (13)

Recent publications for all applications. Explore the full list and refine your search

Life science alliance 7: PubMed38499328

2024

A genetic screen to uncover mechanisms underlying lipid transfer protein function at membrane contact sites.

Applications

Unspecified application

Species

Unspecified reactive species

Shirish Mishra,Vaishnavi Manohar,Shabnam Chandel,Tejaswini Manoj,Subhodeep Bhattacharya,Nidhi Hegde,Vaisaly R Nath,Harini Krishnan,Corinne Wendling,Thomas Di Mattia,Arthur Martinet,Prasanth Chimata,Fabien Alpy,Padinjat Raghu

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2306298 PubMed38064101

2023

BHLHE40 Inhibits Ferroptosis in Pancreatic Cancer Cells via Upregulating SREBF1.

Applications

Unspecified application

Species

Unspecified reactive species

Yizhi Cao,Xuelong Wang,Yang Liu,Pengyi Liu,Jiejie Qin,Youwei Zhu,Shuyu Zhai,Yongsheng Jiang,Yihao Liu,Lijie Han,Jiaxin Luo,Ronghao Zhang,Minmin Shi,Liwen Wang,Xiaomei Tang,Meilin Xue,Jia Liu,Weishen Wang,Chenlei Wen,Xiaxing Deng,Chenghong Peng,Hao Chen,Dongfeng Cheng,Lingxi Jiang,Baiyong Shen

mSphere 8:e0010423 PubMed37017523

2023

Host Lipid Transport Protein ORP1 Is Necessary for Coxiella burnetii Growth and Vacuole Expansion in Macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Baleigh Schuler,Margaret Sladek,Stacey D Gilk

Nature communications 12:5354 PubMed34504082

2021

ORP1L mediated PI(4)P signaling at ER-lysosome-mitochondrion three-way contact contributes to mitochondrial division.

Applications

Unspecified application

Species

Unspecified reactive species

Maxime Boutry,Peter K Kim

Scientific reports 10:8663 PubMed32457374

2020

2-Hydroxypropyl-gamma-cyclodextrin overcomes NPC1 deficiency by enhancing lysosome-ER association and autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Ashutosh Singhal,Evan S Krystofiak,W Gray Jerome,Byeongwoon Song

Molecular biology of the cell 31:793-802 PubMed32023146

2020

Oxysterol-binding protein-related protein 1 variants have opposing cholesterol transport activities from the endolysosomes.

Applications

Unspecified application

Species

Unspecified reactive species

Kexin Zhao,Jason Foster,Neale D Ridgway

Nature communications 10:829 PubMed30783101

2019

Allosteric enhancement of ORP1-mediated cholesterol transport by PI(4,5)P/PI(3,4)P.

Applications

Unspecified application

Species

Unspecified reactive species

Jiangqing Dong,Ximing Du,Huan Wang,Jue Wang,Chang Lu,Xiang Chen,Zhiwen Zhu,Zhipu Luo,Li Yu,Andrew J Brown,Hongyuan Yang,Jia-Wei Wu

Cell reports 25:3180-3193.e7 PubMed30540949

2018

IP Receptors Preferentially Associate with ER-Lysosome Contact Sites and Selectively Deliver Ca to Lysosomes.

Applications

Unspecified application

Species

Unspecified reactive species

Peace Atakpa,Nagendra Babu Thillaiappan,Stefania Mataragka,David L Prole,Colin W Taylor

EMBO reports 19: PubMed29858488

2018

Identification of MOSPD2, a novel scaffold for endoplasmic reticulum membrane contact sites.

Applications

Unspecified application

Species

Unspecified reactive species

Thomas Di Mattia,Léa P Wilhelm,Souade Ikhlef,Corinne Wendling,Danièle Spehner,Yves Nominé,Francesca Giordano,Carole Mathelin,Guillaume Drin,Catherine Tomasetto,Fabien Alpy

Cell reports 19:1807-1818 PubMed28564600

2017

Oxysterol-Binding Protein-Related Protein 1L Regulates Cholesterol Egress from the Endo-Lysosomal System.

Applications

Unspecified application

Species

Unspecified reactive species

Kexin Zhao,Neale D Ridgway

View all publications

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重组Anti-ORP1抗体[EPR8646]