AB192998

Anti-ORP-3 抗体

Name: ORP-3抗体 (ab192998)| Abcam中文官网
Brand: Abcam Limited
SKU: AB192998
Price: 4514 CNY
Availability: InStock

Anti-ORP-3 antibody

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(1 Publication)

Rabbit Polyclonal ORP-3 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human OSBPL3 aa 450-550.

查看别名

KIAA0704, ORP3, OSBP3, OSBPL3, Oxysterol-binding protein-related protein 3, ORP-3, OSBP-related protein 3

2 Images

Supplier Data

Immunoprecipitation - Anti-ORP-3 antibody (AB192998)

Detection of ORP-3 in immunoprecipitates of 293T whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) using ab192998 at 6 μg/mg lysate for IP and at 1 μg/ml for subsequent Western blot detection (lane 1). Lane 2 represents control IgG IP.
Detection : Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-ORP-3 antibody (ab192998)

Predicted band size: 101 kDa

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Supplier Data

Western blot - Anti-ORP-3 antibody (AB192998)

Lysates prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-ORP-3 antibody (ab192998) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 50 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Lane 4:

TCMK1 whole cell lysate at 50 µg

Lane 5:

NIH 3T3 whole cell lysate at 50 µg

Predicted band size: 101 kDa

true

Exposure time: 3min

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Mouse, Human

应用

IP, WB

applications

免疫原

Synthetic Peptide within Human OSBPL3 aa 450-550. The exact immunogen used to generate this antibody is proprietary information.

Q9H4L5

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

纯化说明

ab192998 was affinity purified using an epitope specific to ORP-3 immobilized on solid support.

存储溶液

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

ORP-3 also known as Oxysterol-binding protein-related protein 3 is a member of the oxysterol-binding protein family. It has a mass of approximately 100 kDa and is broadly expressed in a variety of tissues including the liver and spleen. ORP-3 contains a pleckstrin homology domain and an oxysterol-binding domain enabling its interactions with lipids and membranes. These structural aspects allow ORP-3 to have a role in lipid transport and cellular signaling.

Biological function summary

The protein contributes significantly to the regulation of lipid metabolism and cellular homeostasis. It is part of a complex that modulates lipid transport and signaling pathways affecting cellular growth and proliferation. ORP-3's interaction with phosphoinositides is particularly important for maintaining membrane integrity and function. These activities can influence cell movement and positioning which have implications in cellular responses to external stimuli.

Pathways

The protein interacts with several key pathways including the phosphoinositide signaling pathway and the sterol regulatory element-binding protein (SREBP) pathway. In the phosphoinositide pathway ORP-3 interacts with proteins like phosphatidylinositol 4-phosphate to facilitate lipid transport processes. In the SREBP pathway it indirectly influences cholesterol and fatty acid metabolism. These pathways position ORP-3 as an integral component in lipid homeostasis and signaling networks.

Disruptions in ORP-3 function link to lipid-related diseases such as atherosclerosis and certain cancers. In atherosclerosis ORP-3 contributes to cholesterol metabolism potentially affecting plaque formation. Its role in cancers involves its impact on cell proliferation and migration with connections to proteins such as Akt involved in cellular survival pathways. Understanding these associations helps in targeting ORP-3 for therapeutic interventions in these conditions.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Phosphoinositide-binding protein which associates with both cell and endoplasmic reticulum (ER) membranes (PubMed : 16143324). Can bind to the ER membrane protein VAPA and recruit VAPA to plasma membrane sites, thus linking these intracellular compartments (PubMed : 25447204). The ORP3-VAPA complex stimulates RRAS signaling which in turn attenuates integrin beta-1 (ITGB1) activation at the cell surface (PubMed : 18270267, PubMed : 25447204). With VAPA, may regulate ER morphology (PubMed : 16143324). Has a role in regulation of the actin cytoskeleton, cell polarity and cell adhesion (PubMed : 18270267). Binds to phosphoinositides with preference for PI(3,4)P2 and PI(3,4,5)P3 (PubMed : 16143324). Also binds 25-hydroxycholesterol and cholesterol (PubMed : 17428193).

See full target information OSBPL3

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of experimental & clinical cancer research : CR 43:29 PubMed38263248

2024

HIF-2α/LINC02609/APOL1-mediated lipid storage promotes endoplasmic reticulum homeostasis and regulates tumor progression in clear-cell renal cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Haibing Xiao,Yan Qu,Haolin Li,Yi Zhang,Mintian Fei,Chaozhao Liang,Hongmei Yang,Xiaoping Zhang

View all publications

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Anti-ORP-3 抗体

Anti-ORP-3 antibody

Immunoprecipitation - Anti-ORP-3 antibody (AB192998)

Western blot - Anti-ORP-3 antibody (AB192998)