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AB262865

Anti-Ornithine Carbamoyltransferase/OTC抗体[CL4046]

Anti-Ornithine Carbamoyltransferase/OTC antibody [CL4046]

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Mouse Monoclonal Ornithine Carbamoyltransferase/OTC antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Ornithine transcarbamylase, mitochondrial aa 150-300.

查看别名

OTCase, OTC

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ornithine Carbamoyltransferase/OTC antibody [CL4046] (AB262865)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ornithine Carbamoyltransferase/OTC antibody [CL4046] (AB262865)

Paraffin-embedded human small intestine tissue stained for Ornithine Carbamoyltransferase/OTC using ab262865 at 1/5000 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ornithine Carbamoyltransferase/OTC antibody [CL4046] (AB262865)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ornithine Carbamoyltransferase/OTC antibody [CL4046] (AB262865)

Paraffin-embedded human endometrium tissue stained for Ornithine Carbamoyltransferase/OTC using ab262865 at 1/5000 dilution in immunohistochemical analysis. No expression, as expected.

Immunocytochemistry/ Immunofluorescence - Anti-Ornithine Carbamoyltransferase/OTC antibody [CL4046] (AB262865)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Ornithine Carbamoyltransferase/OTC antibody [CL4046] (AB262865)

PFA-fixed, Triton X-100 permeabilized RH-30 cells stained for Ornithine Carbamoyltransferase/OTC (green) using ab262865 at 4 μg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ornithine Carbamoyltransferase/OTC antibody [CL4046] (AB262865)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ornithine Carbamoyltransferase/OTC antibody [CL4046] (AB262865)

Paraffin-embedded human liver tissue stained for Ornithine Carbamoyltransferase/OTC using ab262865 at 1/5000 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ornithine Carbamoyltransferase/OTC antibody [CL4046] (AB262865)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ornithine Carbamoyltransferase/OTC antibody [CL4046] (AB262865)

Paraffin-embedded human duodenum tissue stained for Ornithine Carbamoyltransferase/OTC using ab262865 at 1/5000 dilution in immunohistochemical analysis.

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

CL4046

亚型

IgG1

不含载体蛋白

No

反应种属

Human

应用

IHC-P, ICC/IF, WB

applications

免疫原

Recombinant Fragment Protein within Human Ornithine transcarbamylase, mitochondrial aa 150-300. The exact immunogen used to generate this antibody is proprietary information.

P00480

表位

Binds to an epitope located within the peptide sequence GYEPDASVTKLAEQY as determined by overlapping synthetic peptides.

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/5000 - 1/10000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-4 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100</p>" } } }

性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Ornithine Carbamoyltransferase (OTC) also known as ornithine aminotransferase is an enzyme critical in the urea cycle. The OTC protein facilitates the conversion of ornithine and carbamoyl phosphate into citrulline a process essential for nitrogen excretion. The human OTC protein has a mass of approximately 36 kilodaltons and is primarily expressed in the liver mitochondria. Protein assays and procurement efforts often focus on obtaining accurate measurements and ensuring high-purity OTC peptides enabling proper functional studies.
Biological function summary

Ornithine carbamoyltransferase plays a significant role in detoxifying ammonia by forming citrulline an important urea cycle intermediate. The protein functions as a homotrimer meaning three identical subunits form an active enzyme complex. This multimeric state facilitates efficient substrate processing allowing effective handling of nitrogen waste. The integral role of OTC in the urea cycle highlights its importance in maintaining systemic nitrogen balance.

Pathways

OTC is a central component of the urea cycle and influences nitrogen metabolism pathways. These pathways include the conversion of ammonia to urea an important detoxifying process for safe excretion by the kidneys. OTC closely interacts with enzymes such as argininosuccinate synthetase highlighting the coordinated action required to advance urea formation. Understanding OTC's participation within these pathways helps identify its regulatory points and the potential impact of genetic variations.

Mutations in the OTC gene can lead to ornithine transcarbamylase deficiency a disorder affecting ammonia detoxification. This X-linked genetic condition disrupts normal nitrogen metabolism causing ammonia accumulation particularly harmful to the central nervous system. OTC deficiency shares a functional relationship with other urea cycle disorders such as citrullinemia due to its contribution to the same biochemical pathway. Researchers focus on identifying OTC protein mutations to improve diagnosis and treatment of these conditions.

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

靶点信息

Catalyzes the second step of the urea cycle, the condensation of carbamoyl phosphate with L-ornithine to form L-citrulline (PubMed : 2556444, PubMed : 6372096, PubMed : 8112735). The urea cycle ensures the detoxification of ammonia by converting it to urea for excretion (PubMed : 2556444).
See full target information Ornithine transcarbamylase, mitochondrial

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