AB273435

Anti-SARS-CoV-2 orf7a蛋白抗体[3C9]

Name: ORF7a Protein抗体[3C9] (ab273435)| Abcam中文官网
Brand: Abcam Limited
SKU: AB273435
Price: 4863 CNY
Availability: InStock

Anti-SARS-CoV-2 orf7a antibody [3C9]

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Mouse Monoclonal NS7A antibody. Carrier free. Suitable for WB, ICC/IF and reacts with SARS-CoV-2 samples.

查看别名

ORF7a protein, Accessory protein 7a, Protein U122, Protein X4, 7a

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-SARS-CoV-2 orf7a antibody [3C9] (AB273435)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SARS-CoV-2 orf7a antibody [3C9] (AB273435)

Immunofluorescence analysis of Vero E6 (african green monkey kidney epithelial cell line) cells labelling Protein 7a (green) using ab273435. Red : MitoTracker. Left Panel is merged images.

Lab

Western blot - Anti-SARS-CoV-2 orf7a antibody [3C9] (AB273435)

False colour image of Western blot : Anti-ORF7a Protein antibody [3C9] - Coronavirus staining at 1/1000 dilution, shown in green; Rabbit anti-alpha Tubulin antibody [EP1332Y] (ab52866) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab273435 was shown to bind specifically to ORF7a Protein. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4 Â°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L (IRDye^Â® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye^Â® 680RD) preabsorbed (ab216777) at 1/20000 dilution.

All lanes:

Western blot - Anti-SARS-CoV-2 orf7a antibody [3C9] (ab273435) at 1/1000 dilution

All lanes:

Recombinant SARS-CoV2 ORF7a Fc chimera protein cell lysate at 0.5 µg

Observed band size: 40 kDa

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Supplier Data

Western blot - Anti-SARS-CoV-2 orf7a antibody [3C9] (AB273435)

All lanes:

Western blot - Anti-SARS-CoV-2 orf7a antibody [3C9] (ab273435) at 1/500 dilution

Lane 1:

SARS-CoV-infected Vero E6 (african green monkey kidney epithelial cell line) whole cell lysate

Lane 2:

8 hours after SARS-CoV-infected Vero E6 whole cell lysate

Lane 3:

16 hours after SARS-CoV-infected Vero E6 whole cell lysate

Lane 4:

24 hours after SARS-CoV-infected Vero E6 whole cell lysate

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Supplier Data

Western blot - Anti-SARS-CoV-2 orf7a antibody [3C9] (AB273435)

All lanes:

Western blot - Anti-SARS-CoV-2 orf7a antibody [3C9] (ab273435) at 1/1000 dilution

Lane 1:

Mock, Caco2 (human colorectal adenocarcinoma cell line) whole cell lysate

Lane 2:

SARS-CoV infected, Caco2 whole cell lysate

Lane 3:

SARS-CoV-2, Caco2 whole cell lysate

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不同偶联物与剂型 (1)

Unconjugated

Anti-ATP-binding cassette sub-family A member 3 antibody [3C9]

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

3C9

亚型

IgG1

不含载体蛋白

Yes

反应种属

SARS-CoV-2

应用

WB, ICC/IF

applications

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "SARS-CoV-2": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein G

存储溶液

pH: 7.4 Constituents: PBS

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The ORF7a protein also known as accessory protein 7a acts as an important virulence factor in SARS-CoV-2 the virus causing COVID-19. The protein has a molecular mass of approximately 12 kDa and is expressed in the endoplasmic reticulum. It extends into the lumen where it can bind to proteins and other molecules. ORF7a is known to interact with different host proteins manipulating cellular machinery to aid viral replication and pathogenesis.

Biological function summary

Functions of the ORF7a protein extend to immune system inhibition. It can interfere with type I interferon signaling reducing the host's antiviral response. As part of viral assembly ORF7a contributes to the efficient packaging and release of viral particles. It does not form a complex with other viral proteins but acts independently to execute its functions within the infected host cell.

Pathways

Roles of the ORF7a protein integrate into immune escape and virion production processes. It participates in immune evasion pathways by inhibiting signaling pathways that interfere with interferon production like the JAK-STAT pathway. Additionally it has associations with other SARS-CoV-2 proteins like ORF3b which jointly compromise host cell functions and enhance virus survival.

The ORF7a protein has strong connections to the manifestation of COVID-19-related symptoms. The inhibition of interferon signaling contributes to respiratory distress observed in severe cases of COVID-19. Studies also indicate links to the inflammatory responses involving other viral proteins such as the N protein which together can exacerbate the severity of SARS-CoV-2 infections.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Plays a role as antagonist of host tetherin (BST2), disrupting its antiviral effect. Acts by binding to BST2 thereby interfering with its glycosylation. May suppress small interfering RNA (siRNA). May bind to host ITGAL, thereby playing a role in attachment or modulation of leukocytes.

See full target information 7a

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Anti-SARS-CoV-2 orf7a蛋白抗体[3C9]