重组Anti-Orai2抗体[EPR10043(2)] - C-terminal (ab180146)
![Flow Cytometry (Intracellular) - Anti-Orai2 antibody [EPR10043(2)] - C-terminal (ab180146)](https://www.abcam.cn/ps/products/180/ab180146/Images/ab180146-210037-anti-orai2-antibody-epr100432-c-terminal-flow-cytometry.jpg "Flow Cytometry (Intracellular) - Anti-Orai2 antibody [EPR10043(2)] - C-terminal (ab180146)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10043(2)] to Orai2 - C-terminal
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Orai2抗体[EPR10043(2)] - C-terminal
参阅全部 Orai2 一抗 -
描述
兔单克隆抗体[EPR10043(2)] to Orai2 - C-terminal -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- HeLa and MDA-MB-435 cell lysates; HeLa cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR10043(2) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab180146于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
1/200.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB | (1) |
1/5000 - 1/10000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).
|
| 说明 |
|---|
|
Flow Cyt (Intra)
1/200. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/5000 - 1/10000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa). |
靶标
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功能
Ca(2+) release-activated Ca(2+)-like (CRAC-like) channel subunit which mediates Ca(2+) influx and increase in Ca(2+)-selective current by synergy with the Ca(2+) sensor, STIM1. -
序列相似性
Belongs to the Orai family. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 80228 Human
- Entrez Gene: 269717 Mouse
- Entrez Gene: 304592 Rat
- Omim: 610929 Human
- SwissProt: Q96SN7 Human
- SwissProt: Q8BH10 Mouse
- Unigene: 363308 Human
- Unigene: 308396 Mouse
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别名
- C7orf19 antibody
- CAP binding protein complex interacting protein 2 antibody
- CAP-binding protein complex-interacting protein 2 antibody
see all
图片
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Flow Cytometry (Intracellular) - Anti-Orai2 antibody [EPR10043(2)] - C-terminal (ab180146)
Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling Orai2 with ab180146 at 1/200 dilution (red). Secondary antibody Goat anti rabbit IgG (FITC) at 1/75 dilution. Isotype control Rabbit IgG (green).
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![Western blot - Anti-Orai2 antibody [EPR10043(2)] - C-terminal (ab180146)](https://www.abcam.cn/ps/products/180/ab180146/Images/ab180146-210036-anti-orai2-antibody-epr100432-c-terminal-western-blot.jpg)
Western blot - Anti-Orai2 antibody [EPR10043(2)] - C-terminal (ab180146)All lanes : Anti-Orai2 antibody [EPR10043(2)] - C-terminal (ab180146) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : MDA-MB-435 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 29 kDa -
![Anti-Orai2 antibody [EPR10043(2)] - C-terminal (ab180146)](https://www.abcam.cn/ps/products/180/ab180146/Images/ab180146-3-benefits-of-recombinant-antibodies.png)
Anti-Orai2 antibody [EPR10043(2)] - C-terminal (ab180146)
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (5)
ab180146 被引用在 5 文献中.
- Tanwar J et al. MITF is a novel transcriptional regulator of the calcium sensor STIM1: Significance in physiological melanogenesis. J Biol Chem 298:102681 (2022). PubMed: 36356899
- Li X et al. CRAC Channel Controls the Differentiation of Pathogenic B Cells in Lupus Nephritis. Front Immunol 12:779560 (2021). PubMed: 34745151
- Arora S et al. Orai3 Regulates Pancreatic Cancer Metastasis by Encoding a Functional Store Operated Calcium Entry Channel. Cancers (Basel) 13:N/A (2021). PubMed: 34885048
- Nascimento Da Conceicao V et al. Resolving macrophage polarization through distinct Ca2+ entry channel that maintains intracellular signaling and mitochondrial bioenergetics. iScience 24:103339 (2021). PubMed: 34816101
- Moon H et al. Crbn modulates calcium influx by regulating Orai1 during efferocytosis. Nat Commun 11:5489 (2020). PubMed: 33127885