Anti-Orai1抗体[266.1] (ab175040)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Orai1 antibody [266.1] (ab175040)](https://www.abcam.cn/ps/products/175/ab175040/Images/ab175040-333645-anti-orai1-antibody-2661-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Orai1 antibody [266.1] (ab175040)")
Key features and details
- Mouse monoclonal [266.1] to Orai1
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
概述
-
产品名称
Anti-Orai1抗体[266.1]
参阅全部 Orai1 一抗 -
描述
小鼠单克隆抗体[266.1] to Orai1 -
宿主
Mouse -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Rat, Human
预测可用于: Mouse, Cynomolgus monkey
-
免疫原
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
-
阳性对照
- This antibody gave a positive signal in human and rat kidney, and human and rat testis
-
常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading... -
纯度
Protein G purified -
克隆
单克隆 -
克隆编号
266.1 -
同种型
IgG -
研究领域
相关产品
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab175040于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
Use a concentration of 5 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 35 kDa).
|
| IHC-P |
Use a concentration of 0.5 µg/ml.
|
| 说明 |
|---|
|
WB
Use a concentration of 5 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 35 kDa). |
|
IHC-P
Use a concentration of 0.5 µg/ml. |
靶标
-
功能
Ca(2+) release-activated Ca(2+) (CRAC) channel subunit which mediates Ca(2+) influx following depletion of intracellular Ca(2+) stores and channel activation by the Ca(2+) sensor, STIM1. CRAC channels are the main pathway for Ca(2+) influx in T-cells and promote the immune response to pathogens by activating the transcription factor NFAT. -
疾病相关
Defects in ORAI1 are the cause of immune dysfunction with T-cell inactivation due to calcium entry defect type 1 (IDTICED1) [MIM:612782]. IDTICED1 is an immune disorder characterized by recurrent infections, impaired T-cell activation and proliferative response, decreased T-cell production of cytokines, and normal lymphocytes counts and serum immunoglobulin levels. In surviving patients ectodermal dysplasia with anhydrosis and non-progressive myopathy may be observed. -
序列相似性
Belongs to the Orai family. -
细胞定位
Cell membrane. - Information by UniProt
-
数据库链接
- Entrez Gene: 84876 Human
- Entrez Gene: 109305 Mouse
- Entrez Gene: 304496 Rat
- Omim: 610277 Human
- SwissProt: Q96D31 Human
- SwissProt: Q8BWG9 Mouse
- SwissProt: Q5M848 Rat
- Unigene: 55148 Human
see all -
别名
- Calcium release activated calcium channel protein 1 antibody
- Calcium release activated calcium modulator 1 antibody
- Calcium release-activated calcium channel protein 1 antibody
see all
图片
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Orai1 antibody [266.1] (ab175040)IHC image of Orai1 staining in human kidney formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab175040, 0.5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
-
![Western blot - Anti-Orai1 antibody [266.1] (ab175040)](https://www.abcam.cn/ps/products/175/ab175040/Images/ab175040-333644-anti-orai1-antibody-2661-western-blot.jpg)
Western blot - Anti-Orai1 antibody [266.1] (ab175040)All lanes : Anti-Orai1 antibody [266.1] (ab175040) at 5 µg/ml
Lane 1 : Human kidney tissue lysate - total protein (ab30203)
Lane 2 : Rat Kidney Tissue Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 35 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?
Additional bands at: 15 kDa, 32 kDa, 39 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes -
![Western blot - Anti-Orai1 antibody [266.1] (ab175040)](https://www.abcam.cn/ps/products/175/ab175040/Images/ab175040-333643-anti-orai1-antibody-2661-western-blot.jpg)
Western blot - Anti-Orai1 antibody [266.1] (ab175040)Anti-Orai1 antibody [266.1] (ab175040) at 5 µg/ml + Testis (Rat) Tissue Lysate (ab4040) at 30 µg
Secondary
Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 35 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?
Additional bands at: 32 kDa, 39 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
数据表及文件
-
SDS download
-
Datasheet download
文献 (9)
ab175040 被引用在 9 文献中.
- Novakovic MM et al. Astrocyte reactivity and inflammation-induced depression-like behaviors are regulated by Orai1 calcium channels. Nat Commun 14:5500 (2023). PubMed: 37679321
- Wang WA & Demaurex N The mammalian trafficking chaperone protein UNC93B1 maintains the ER calcium sensor STIM1 in a dimeric state primed for translocation to the ER cortex. J Biol Chem 298:101607 (2022). PubMed: 35065962
- Li M et al. Intracellular Ca2+ Signaling and Calcium Release-Activated Calcium Modulator ORAI1 Are Associated With CD4+ T Lymphocytes in Dairy Cows. Front Immunol 13:835936 (2022). PubMed: 35663942
- Chen X et al. Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels. J Extracell Vesicles 11:e12279 (2022). PubMed: 36482876
- Galeano-Otero I et al. SARAF and Orai1 Contribute to Endothelial Cell Activation and Angiogenesis. Front Cell Dev Biol 9:639952 (2021). PubMed: 33748129
- Hogea A et al. Junctophilin-4 facilitates inflammatory signalling at plasma membrane-endoplasmic reticulum junctions in sensory neurons. J Physiol 599:2103-2123 (2021). PubMed: 33569781
- Zhou Z et al. Store-Operated Calcium Channels Contribute to Remifentanil-Induced Postoperative Hyperalgesia via Phosphorylation of CaMKIIα in Rats. J Pain Res 14:3289-3299 (2021). PubMed: 34703304
- Maneshi MM et al. Orai1 Channels Are Essential for Amplification of Glutamate-Evoked Ca2+ Signals in Dendritic Spines to Regulate Working and Associative Memory. Cell Rep 33:108464 (2020). IHC-P ; Mouse . PubMed: 33264616
- Guzman R et al. Expression of ORAI1, a Plasma Membrane Resident Subunit of the CRAC Channel, in Rodent and Non-rodent Species. J Histochem Cytochem : (2014). PubMed: 25249026