Anti-Optineurin抗体(ab23666)
Key features and details
- Rabbit polyclonal to Optineurin
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Optineurin抗体
参阅全部 Optineurin 一抗 -
描述
兔多克隆抗体to Optineurin -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Cow, Pig, Cynomolgus monkey, Macaque monkey
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免疫原
Synthetic peptide. This information is considered to be commercially sensitive.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Constituents: 66% Sodium chloride, 25% Tris -
Concentration information loading... -
纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab23666于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (4) |
1/200. Detects a band of approximately 75 kDa (predicted molecular weight: 74 kDa).
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| 说明 |
|---|
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WB
1/200. Detects a band of approximately 75 kDa (predicted molecular weight: 74 kDa). |
靶标
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功能
Plays an important role in the maintenance of the Golgi complex, in membrane trafficking, in exocytosis, through its interaction with myosin VI and Rab8. Links myosin VI to the Golgi complex and plays an important role in Golgi ribbon formation. Negatively regulates the induction of IFNB in response to RNA virus infection. Plays a neuroprotective role in the eye and optic nerve. Probably part of the TNF-alpha signaling pathway that can shift the equilibrium toward induction of cell death. May act by regulating membrane trafficking and cellular morphogenesis via a complex that contains Rab8 and hungtingtin (HD). May constitute a cellular target for adenovirus E3 14.7, an inhibitor of TNF-alpha functions, thereby affecting cell death. -
组织特异性
Present in acqueous humor of the eye (at protein level). Highly expressed in trabecular meshwork. Expressed nonpigmented ciliary epithelium, retina, brain, adrenal cortex, fetus, lymphocyte, fibroblast, skeletal muscle, heart, liver, brain and placenta. -
疾病相关
Defects in OPTN are the cause of primary open angle glaucoma type 1E (GLC1E) [MIM:137760]. Primary open angle glaucoma (POAG) is characterized by a specific pattern of optic nerve and visual field defects. The angle of the anterior chamber of the eye is open, and usually the intraocular pressure is increased. The disease is asymptomatic until the late stages, by which time significant and irreversible optic nerve damage has already taken place.
Defects in OPTN are a cause of susceptibility to normal pressure glaucoma (NPG) [MIM:606657].
Defects in OPTN are the cause of amyotrophic lateral sclerosis type 12 (ALS12) [MIM:613435]. It is a neurodegenerative disorder affecting upper motor neurons in the brain and lower motor neurons in the brain stem and spinal cord, resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of the cases. -
结构域
Ubiquitin-binding motif (UBAN) is essential for its inhibitory function, subcellular localization and interaction with TBK1. -
翻译后修饰
Phosphorylated. Phosphorylation is induced by phorbol esters and decreases its half-time. -
细胞定位
Cytoplasm > perinuclear region. Golgi apparatus. Golgi apparatus > trans-Golgi network. Found in the perinuclear region and associates with the Golgi apparatus. Colocalizes with MYO6 and RAB8 at the Golgi complex and in vesicular structures close to the plasma membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 10133 Human
- Entrez Gene: 71648 Mouse
- Entrez Gene: 397011 Pig
- Entrez Gene: 246294 Rat
- Omim: 602432 Human
- SwissProt: Q95KA2 Cynomolgus monkey
- SwissProt: Q96CV9 Human
- SwissProt: Q8K3K8 Mouse
see all -
别名
- 14.7K interacting protein antibody
- Ag9 C5 antibody
- ALS12 antibody
see all
图片
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Western blot - Anti-Optineurin antibody (ab23666)Anti-Optineurin antibody (ab23666) at 1 µg + Human skeletal muscle tissue lysate - total protein at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 74 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Additional bands at: 24 kDa, 43 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute
数据表及文件
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Datasheet download
文献 (48)
ab23666 被引用在 48 文献中.
- Kataura T et al. NDP52 acts as a redox sensor in PINK1/Parkin-mediated mitophagy. EMBO J 42:e111372 (2023). PubMed: 36514953
- Robinson CA et al. Kaposi's sarcoma-associated herpesvirus (KSHV) utilizes the NDP52/CALCOCO2 selective autophagy receptor to disassemble processing bodies. PLoS Pathog 19:e1011080 (2023). PubMed: 36634147
- Goldsmith J et al. Brain-derived autophagosome profiling reveals the engulfment of nucleoid-enriched mitochondrial fragments by basal autophagy in neurons. Neuron 110:967-976.e8 (2022). PubMed: 35051374
- Lee YJ et al. Chemical modulation of SQSTM1/p62-mediated xenophagy that targets a broad range of pathogenic bacteria. Autophagy 18:2926-2945 (2022). PubMed: 35316156
- Moharir SC & Swarup G Optineurin deficiency induces patchy hair loss but it is not sufficient to cause amyotrophic lateral sclerosis in mice. Biochim Biophys Acta Mol Basis Dis 1868:166470 (2022). PubMed: 35750266
