Anti-OPN3抗体(ab75285)
Key features and details
- Rabbit polyclonal to OPN3
- Suitable for: ELISA, WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-OPN3抗体
参阅全部 OPN3 一抗 -
描述
兔多克隆抗体to OPN3 -
宿主
Rabbit -
经测试应用
适用于: ELISA, WB, ICC/IFmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide corresponding to Human OPN3 (internal sequence).
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阳性对照
- WB: Extracts from mouse brain cells. ICC/IF: MCF-7 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
The antibody was affinity purified from rabbit antiserum by affinity chromatography -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab75285于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
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| WB |
1/500 - 1/1000. Predicted molecular weight: 45 kDa.
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| ICC/IF |
1/500 - 1/1000.
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| 说明 |
|---|
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ELISA
Use at an assay dependent concentration. |
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WB
1/500 - 1/1000. Predicted molecular weight: 45 kDa. |
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ICC/IF
1/500 - 1/1000. |
靶标
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功能
May play a role in encephalic photoreception. -
组织特异性
Strongly expressed in brain. Highly expressed in the preoptic area and paraventricular nucleus of the hypothalamus. Shows highly patterned expression in other regions of the brain, being enriched in selected regions of the cerebral cortex, cerebellar Purkinje cells, a subset of striatal neurons, selected thalamic nuclei, and a subset of interneurons in the ventral horn of the spinal cord. -
序列相似性
Belongs to the G-protein coupled receptor 1 family. Opsin subfamily. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 23596 Human
- Entrez Gene: 13603 Mouse
- Omim: 606695 Human
- SwissProt: Q9H1Y3 Human
- SwissProt: Q9WUK7 Mouse
- Unigene: 409081 Human
- Unigene: 32744 Mouse
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别名
- ECPN antibody
- Encephalopsin antibody
- ERO antibody
see all
图片
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Western blot - Anti-OPN3 antibody (ab75285)All lanes : Anti-OPN3 antibody (ab75285) at 1/500 dilution
Lane 1 : Extracts from mouse brain cells
Lane 2 : Extracts from mouse brain cells with immunising peptide at 10 µg
Lysates/proteins at 10 µg per lane.
Predicted band size: 45 kDa
Observed band size: 45 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-OPN3 antibody (ab75285)ab75285 staining OPN3 in MCF-7 cells by Immunofluorescence. Image on right when blocking peptide added.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (12)
ab75285 被引用在 12 文献中.
- Chen H et al. MiR-22-3p Suppresses Vascular Remodeling and Oxidative Stress by Targeting CHD9 during the Development of Hypertension. J Vasc Res 58:180-190 (2021). PubMed: 33794525
- Konishi H et al. A tumor-specific modulation of heterogeneous ribonucleoprotein A0 promotes excessive mitosis and growth in colorectal cancer cells. Cell Death Dis 11:245 (2020). PubMed: 32303675
- Xia T et al. miR-29b promotes the osteogenic differentiation of mesenchymal stem cells derived from human adipose tissue via the PTEN/AKT/ß-catenin signaling pathway. Int J Mol Med 46:709-717 (2020). PubMed: 32468003
- Yu W et al. Different effects of total flavonoids from Arachniodes exilis on human umbilical cord mesenchymal stem cells in vitro. Medicine (Baltimore) 99:e20628 (2020). PubMed: 32569193
- Che M et al. Long noncoding RNA HCG18 inhibits the differentiation of human bone marrow-derived mesenchymal stem cells in osteoporosis by targeting miR-30a-5p/NOTCH1 axis. Mol Med 26:106 (2020). PubMed: 33176682