Anti-OPA1抗体(ab42364)
Key features and details
- Rabbit polyclonal to OPA1
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-OPA1抗体
参阅全部 OPA1 一抗 -
描述
兔多克隆抗体to OPA1 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Chicken, Orangutan
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免疫原
Synthetic peptide within Rat OPA1 aa 900 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
(Peptide available asab42363) -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab42364于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (5) |
Use a concentration of 1 µg/ml. Detects a band of approximately 92,86 kDa (predicted molecular weight: 111 kDa).
Abcam recommends using milk as the blocking agent. |
| IP |
Use at an assay dependent concentration.
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| 说明 |
|---|
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 92,86 kDa (predicted molecular weight: 111 kDa). Abcam recommends using milk as the blocking agent. |
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IP
Use at an assay dependent concentration. |
靶标
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功能
Dynamin-related GTPase required for mitochondrial fusion and regulation of apoptosis. May form a diffusion barrier for proteins stored in mitochondrial cristae. Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into the mitochondrial intermembrane space. -
组织特异性
Highly expressed in retina. Also expressed in brain, testis, heart and skeletal muscle. Isoform 1 expressed in retina, skeletal muscle, heart, lung, ovary, colon, thyroid gland, leukocytes and fetal brain. Isoform 2 expressed in colon, liver, kidney, thyroid gland and leukocytes. Low levels of all isoforms expressed in a variety of tissues. -
疾病相关
Defects in OPA1 are a cause of optic atrophy type 1 (OPA1) [MIM:165500]. OPA1 is a dominantly inherited optic neuropathy occurring in 1 in 50,000 individuals that features progressive loss in visual acuity leading, in many cases, to legal blindness.
Defects in OPA1 are the cause of optic atrophy 1 with deafness (OPA1D) [MIM:125250]. Some individuals with mutations in OPA1 manifest also ophthalmoplegia and myopathy. -
序列相似性
Belongs to the dynamin family. -
翻译后修饰
PARL-dependent proteolytic processing releases an antiapoptotic soluble form not required for mitochondrial fusion. -
细胞定位
Mitochondrion inner membrane. Mitochondrion intermembrane space. - Information by UniProt
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数据库链接
- Entrez Gene: 424900 Chicken
- Entrez Gene: 4976 Human
- Entrez Gene: 74143 Mouse
- Entrez Gene: 171116 Rat
- Omim: 605290 Human
- SwissProt: O60313 Human
- SwissProt: P58281 Mouse
- SwissProt: Q2TA68 Rat
see all -
别名
- Dynamin like 120 kDa protein antibody
- Dynamin like 120 kDa protein, mitochondrial antibody
- Dynamin-like 120 kDa protein, form S1 antibody
see all
图片
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Western blot - Anti-OPA1 antibody (ab42364)All lanes : Anti-OPA1 antibody (ab42364) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Brain (Mouse) Tissue Lysate
Lane 3 : Brain (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 111 kDa
Observed band size: 92 kDa why is the actual band size different from the predicted?
Additional bands at: 100 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutesAbcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
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Western blot - Anti-OPA1 antibody (ab42364)Anti-OPA1 antibody (ab42364) at 1 µg/ml + Brain (Rat) Tissue Lysate - normal tissue at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 111 kDa
Observed band size: 92 kDa why is the actual band size different from the predicted?
Additional bands at: 86 kDa (possible isoform)
Although the predicted band size is ~111 kDa based on Swiss-prot data, bands of 92 kDa and 86 kDa has been previously observed. Investigative Ophthalmology & Visual Science, November 2005, Vol. 46, No. 11 -
Immunoprecipitation - Anti-OPA1 antibody (ab42364)OPA1 was immunoprecipitated using 0.5mg Rat Brain whole tissue lysate, 5µg of Rabbit polyclonal to OPA1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, rat brain whole tissue lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab42364.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 92kDa: OPA1; non specific - 100kDa: We are unsure as to the identity of this extra band.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (125)
ab42364 被引用在 125 文献中.
- Zhang W et al. Astragaloside IV Alleviates Infarction Induced Cardiomyocyte Injury by Improving Mitochondrial Morphology and Function. Front Cardiovasc Med 9:810541 (2022). PubMed: 35265681
- Yu Y et al. Exercise-Generated β-Aminoisobutyric Acid (BAIBA) Reduces Cardiomyocyte Metabolic Stress and Apoptosis Caused by Mitochondrial Dysfunction Through the miR-208b/AMPK Pathway. Front Cardiovasc Med 9:803510 (2022). PubMed: 35282369
- Li L et al. Mitochondrial dynamics and biogenesis indicators may serve as potential biomarkers for diagnosis of myasthenia gravis. Exp Ther Med 23:307 (2022). PubMed: 35340870
- Kim YS et al. Tomatidine-stimulated maturation of human embryonic stem cell-derived cardiomyocytes for modeling mitochondrial dysfunction. Exp Mol Med 54:493-502 (2022). PubMed: 35379934
- Li X et al. Ischemia-induced cleavage of OPA1 at S1 site aggravates mitochondrial fragmentation and reperfusion injury in neurons. Cell Death Dis 13:321 (2022). PubMed: 35395832