Anti-OPA1抗体[1E81D9] (ab119685)
![Flow Cytometry - Anti-OPA1 antibody [1E81D9] (ab119685)](https://www.abcam.cn/ps/products/119/ab119685/Images/ab119685-204494-anti-opa1-antibody-1e81d9-flow-cytometry.jpg "Flow Cytometry - Anti-OPA1 antibody [1E81D9] (ab119685)")
Key features and details
- Mouse monoclonal [1E81D9] to OPA1
- Suitable for: WB, Flow Cyt
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
概述
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产品名称
Anti-OPA1抗体[1E81D9]
参阅全部 OPA1 一抗 -
描述
小鼠单克隆抗体[1E81D9] to OPA1 -
宿主
Mouse -
经测试应用
适用于: WB, Flow Cytmore details
不适用于: ELISA or IP -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant fragment. This information is considered to be commercially sensitive.
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常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: 99% HEPES buffered saline -
Concentration information loading... -
纯度
Ammonium Sulphate Precipitation -
纯化说明
Purity is near homogeneity as judged by SDS-PAGE. ab119685 was produced in vitro using hybridomas grown in serum-free medium, and then concentrated by ammonium sulfate precipitation. -
克隆
单克隆 -
克隆编号
1E81D9 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab119685于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
Use a concentration of 1 µg/ml. Predicted molecular weight: 112 kDa.
|
| Flow Cyt |
Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| 说明 |
|---|
|
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 112 kDa. |
|
Flow Cyt
Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
应用说明
Is unsuitable for ELISA or IP.
靶标
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功能
Dynamin-related GTPase required for mitochondrial fusion and regulation of apoptosis. May form a diffusion barrier for proteins stored in mitochondrial cristae. Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into the mitochondrial intermembrane space. -
组织特异性
Highly expressed in retina. Also expressed in brain, testis, heart and skeletal muscle. Isoform 1 expressed in retina, skeletal muscle, heart, lung, ovary, colon, thyroid gland, leukocytes and fetal brain. Isoform 2 expressed in colon, liver, kidney, thyroid gland and leukocytes. Low levels of all isoforms expressed in a variety of tissues. -
疾病相关
Defects in OPA1 are a cause of optic atrophy type 1 (OPA1) [MIM:165500]. OPA1 is a dominantly inherited optic neuropathy occurring in 1 in 50,000 individuals that features progressive loss in visual acuity leading, in many cases, to legal blindness.
Defects in OPA1 are the cause of optic atrophy 1 with deafness (OPA1D) [MIM:125250]. Some individuals with mutations in OPA1 manifest also ophthalmoplegia and myopathy. -
序列相似性
Belongs to the dynamin family. -
翻译后修饰
PARL-dependent proteolytic processing releases an antiapoptotic soluble form not required for mitochondrial fusion. -
细胞定位
Mitochondrion inner membrane. Mitochondrion intermembrane space. - Information by UniProt
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数据库链接
- Entrez Gene: 4976 Human
- Entrez Gene: 74143 Mouse
- Entrez Gene: 171116 Rat
- Omim: 605290 Human
- SwissProt: O60313 Human
- SwissProt: P58281 Mouse
- SwissProt: Q2TA68 Rat
- Unigene: 594504 Human
see all -
别名
- Dynamin like 120 kDa protein antibody
- Dynamin like 120 kDa protein, mitochondrial antibody
- Dynamin-like 120 kDa protein, form S1 antibody
see all
图片
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Flow Cytometry - Anti-OPA1 antibody [1E81D9] (ab119685)Overlay histogram showing SH-SY5Y cells stained with ab119685 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab119685, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
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![Western blot - Anti-OPA1 antibody [1E81D9] (ab119685)](https://www.abcam.cn/ps/products/119/ab119685/Images/ab119685-132834-anti-opa1-antibody-1e81d9-western-blot.jpg)
Western blot - Anti-OPA1 antibody [1E81D9] (ab119685)All lanes : Anti-OPA1 antibody [1E81D9] (ab119685) at 1 µg/ml
Lane 1 : whole cell lysates from HeLa cells(human)
Lane 2 : whole cell lysates from H4IIE cells(rat)
Lane 3 : whole cell lysates from MEF cells(mouse)
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP goat anti-mouse at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 112 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (13)
ab119685 被引用在 13 文献中.
- Stevanović-Silva J et al. Gestational Exercise Antagonises the Impact of Maternal High-Fat High-Sucrose Diet on Liver Mitochondrial Alterations and Quality Control Signalling in Male Offspring. Int J Environ Res Public Health 20:N/A (2023). PubMed: 36674144
- Venediktova N et al. Effect of Thyroxine on the Structural and Dynamic Features of Cardiac Mitochondria and Mitophagy in Rats. Cells 12:N/A (2023). PubMed: 36766738
- Yuan L et al. Matrine alleviates cisplatin-induced acute kidney injury by inhibiting mitochondrial dysfunction and inflammation via SIRT3/OPA1 pathway. J Cell Mol Med 26:3702-3715 (2022). PubMed: 35650472
- Lv XH et al. Anti-diabetic drug canagliflozin hinders skeletal muscle regeneration in mice. Acta Pharmacol Sin 43:2651-2665 (2022). PubMed: 35217814
- Wu P et al. Ginsenoside Rg3 alleviates septic liver injury by regulating the lncRNA TUG1/miR-200c-3p/SIRT1 axis. J Inflamm (Lond) 18:31 (2021). PubMed: 34930287