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重组RabMAb

重组Anti-Olig1抗体[EPR6790] (ab124908)

Submit a review Q&A (1)References (2)
Western blot - Anti-Olig1 antibody [EPR6790] (ab124908)
  • Flow Cytometry (Intracellular) - Anti-Olig1 antibody [EPR6790] (ab124908)
  • OI-RD Scanning - Anti-Olig1 antibody [EPR6790] (ab124908)
  • Anti-Olig1 antibody [EPR6790] (ab124908)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6790] to Olig1
  • Suitable for: Flow Cyt (Intra), WB
  • Reacts with: Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-Olig1抗体[EPR6790]
    参阅全部 Olig1 一抗

  • 描述

    兔单克隆抗体[EPR6790] to Olig1

  • 宿主

    Rabbit

  • 经测试应用

    适用于: Flow Cyt (Intra), WBmore details
    不适用于: ICC/IF,IHC-P or IP

  • 种属反应性

    与反应: Rat, Human
    预测可用于: Mouse

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • MOLT4, SHSY-5Y, 293T and C6 cell lysates; 293T cells

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab124908于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.  
WB
1/1000 - 1/10000. Detects a band of approximately 35 kDa (predicted molecular weight: 27 kDa).
说明
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.  
WB
1/1000 - 1/10000. Detects a band of approximately 35 kDa (predicted molecular weight: 27 kDa).
应用说明
Is unsuitable for ICC/IF,IHC-P or IP.

靶标

  • 功能

    Promotes formation and maturation of oligodendrocytes, especially within the brain. Cooperates with OLIG2 to establish the pMN domain of the embryonic neural tube.

  • 组织特异性

    Expressed in the brain, in oligodendrocytes. Strongly expressed in oligodendrogliomas, while expression is weak to moderate in astrocytomas. Expression in glioblastomas is highly variable.

  • 序列相似性

    Contains 1 basic helix-loop-helix (bHLH) domain.

  • 细胞定位

    Nucleus.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • Basic domain helix loop helix protein class B 6 antibody
    • Basic domain helix loop helix protein class B6 antibody
    • BHLH B6 antibody
    • BHLHB 6 antibody
    • bHLHb6 antibody
    • bHLHe21 antibody
    • Class B basic helix-loop-helix protein 6 antibody
    • Class E basic helix-loop-helix protein 21 antibody
    • Olig 1 antibody
    • Olig1 antibody
    • OLIG1_HUMAN antibody
    • Oligo 1 antibody
    • Oligo1 antibody
    • Oligodendrocyte lineage transcription factor 1 antibody
    • Oligodendrocyte specific bHLH transcription factor 1 antibody
    • Oligodendrocyte transcription factor 1 antibody
    see all

图片

  • Western blot - Anti-Olig1 antibody [EPR6790] (ab124908)
    Western blot - Anti-Olig1 antibody [EPR6790] (ab124908)
    All lanes : Anti-Olig1 antibody [EPR6790] (ab124908) at 1/1000 dilution

    Lane 1 : MOLT4 cell lysate
    Lane 2 : SH-SY5Y cell lysate
    Lane 3 : 293T (Human embryonic kidney epithelial cell) cell lysate
    Lane 4 : C6 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 27 kDa
    Observed band size: 35 kDa why is the actual band size different from the predicted?

  • Flow Cytometry (Intracellular) - Anti-Olig1 antibody [EPR6790] (ab124908)
    Flow Cytometry (Intracellular) - Anti-Olig1 antibody [EPR6790] (ab124908)

    Intracellular flow cytometric analysis of permeabilized 293T cells using ab124908 at 1/10 dilution (red) or a rabbit IgG negative control (green).

     

  • OI-RD Scanning - Anti-Olig1 antibody [EPR6790] (ab124908)
    OI-RD Scanning - Anti-Olig1 antibody [EPR6790] (ab124908)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-Olig1 antibody [EPR6790] (ab124908)
    Anti-Olig1 antibody [EPR6790] (ab124908)

文献 (2)

发表研究结果有使用 ab124908？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab124908 被引用在 2 文献中.

  • Jiang H  et al. Amelioration of experimental autoimmune encephalomyelitis through transplantation of placental derived mesenchymal stem cells. Sci Rep 7:41837 (2017). PubMed: 28186117
  • Cheng T  et al. Effect of OLIG1 on the development of oligodendrocytes and myelination in a neonatal rat PVL model induced by hypoxia-ischemia. Mol Med Rep 11:2379-86 (2015). WB, IHC ; Rat . PubMed: 25435330

客户评价及客户问答

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Question

From the image on your datasheet, the OLIG1 antibodydetects a band at approximately 35 kDa. However, the molecular weight of OLIG1 is found to be only 27 kDa. Would you have any information regarding the difference in molecular weight?

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Answer



Not sure exactly why Olig1 runs a bit slower than would be suggested based on theoretical mass alone. It doesn't appear to be post translationally modified. But another Olig1 antibody in our catalog, ab97507 https://www.abcam.com/Olig1-antibody-ab97507.html) also runs at the same molecular mass.

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