重组Anti-Olfactory Marker蛋白/OMP抗体[EPR19190] (ab183947)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19190] to Olfactory Marker Protein/OMP
- Suitable for: IHC-Fr, IHC-P, WB, IP
- Reacts with: Mouse, Rat
Related conjugates and formulations
概述
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产品名称
Anti-Olfactory Marker蛋白/OMP抗体[EPR19190]
参阅全部 Olfactory Marker Protein/OMP 一抗 -
描述
兔单克隆抗体[EPR19190] to Olfactory Marker蛋白/OMP -
宿主
Rabbit -
经测试应用
适用于: IHC-Fr, IHC-P, WB, IPmore details -
种属反应性
与反应: Mouse, Rat -
免疫原
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Mouse and Rat olfactory bulb lysates. IHC-P: Mouse olfactory bulb tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR19190 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab183947于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-Fr |
1/500.
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IHC-P | (1) |
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Suitable for use in mouse tissue only. |
WB | (1) |
1/2000. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa).
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IP |
1/40.
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说明 |
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IHC-Fr
1/500. |
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. Suitable for use in mouse tissue only. |
WB
1/2000. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa). |
IP
1/40. |
靶标
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相关性
Olfactory marker protein (OMP) is an abundant, 19kDa, cytosolic protein that is almost exclusively expressed in mature, functioning, olfactory neurons but not in the neural precursor basal cells. The OMP gene structure and protein sequence are highly conserved between mouse, rat and human. Its tissue specific expression in the receptor cells together with the results of the mouse knockout studies show that OMP represents a novel modulatory component of the odor detection/signal transduction cascade. -
细胞定位
Cytoplasmic -
数据库链接
- Entrez Gene: 18378 Mouse
- Entrez Gene: 24612 Rat
- SwissProt: Q64288 Mouse
- SwissProt: P08523 Rat
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别名
- Olfactory marker protein antibody
- Olfactory neuronal specific protein antibody
- OMP antibody
图片
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All lanes : Anti-Olfactory Marker Protein/OMP antibody [EPR19190] (ab183947) at 1/2000 dilution
Lane 1 : Mouse olfactory bulb lysate
Lane 2 : Mouse brain lysate
Lane 3 : Mouse heart lysate
Lane 4 : Mouse kidney lysate
Lane 5 : Mouse spleen lysate
Lane 6 : Neuro-2a (Mouse neuroblastoma cell line) lysate
Lane 7 : Rat olfactory bulb lysate
Lane 8 : Rat kidney lysate
Lane 9 : C6 (Rat glial tumor cell line) lysate
Lane 10 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) lysate
Lane 11 : PC-12 (Rat adrenal gland pheochromocytoma cell line) lysate
Lane 12 : NIH/3T3 (Mouse embryo fibroblast cell line) lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 19 kDa
Observed band size: 19 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
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Olfactory Marker Protein/OMP was immunoprecipitated from 1mg of Mouse olfactory bulb whole cell lysate with ab183947 at 1/40 dilution. Western blot was performed using ab183947 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.
Lane 1(input): Mouse olfactory bulb whole cell lysate 10μg
Lane 2: Mouse olfactory bulb whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab183947 in Mouse olfactory bulb whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
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Immunohistochemical analysis of paraffin-embedded Mouse olfactory bulb tissue labeling Olfactory Marker Protein/OMP with ab183947 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasm staining on neurons of mouse olfactory bulb is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Olfactory Marker Protein/OMP with ab183947 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Negative staining on mouse liver is observed.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemistry (Frozen sections) analysis of mouse olfactory bulb tissue labelling Olfactory Marker Protein/OMP with ab183947 at a dilution of 1/500. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG was used as the secondary antibody (1/1000). Staining is visible in the cytoplasm of neurons of the mouse olfactory bulb.
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Immunohistochemistry (Frozen sections) analysis of rat olfactory bulb tissue labelling Olfactory Marker Protein/OMP with ab183947 at a dilution of 1/500. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG was used as the secondary antibody (1/1000). Staining is visible in the cytoplasm of neurons of the rat olfactory bulb.
实验方案
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (20)
ab183947 被引用在 20 文献中.
- Zhang C et al. Odor Enrichment Attenuates the Anesthesia/Surgery-induced Cognitive Impairment. Ann Surg 277:e1387-e1396 (2023). PubMed: 35848747
- Zhou J et al. Zika virus leads to olfactory disorders in mice by targeting olfactory ensheathing cells. EBioMedicine 89:104457 (2023). PubMed: 36739631
- Duan Y et al. Long-Term Flexible Neural Interface for Synchronous Recording of Cross-Regional Sensory Processing along the Olfactory Pathway. Small 19:e2205768 (2023). PubMed: 37035943
- Lu X et al. DMBT1 Alleviates Nasal Airway Inflammatory Response in the LPS-Induced Nasal Polyp Model. Int Arch Allergy Immunol 184:808-813 (2023). PubMed: 37232026
- Fan Y et al. Mapping transgene insertion sites reveals the α-Cre transgene expression in both developing retina and olfactory neurons. Commun Biol 5:411 (2022). PubMed: 35505181