AB251035

重组Anti-OLF1抗体[EPR15972] - BSA and Azide free

Anti-OLF1 antibody [EPR15972] - BSA and Azide free

RabMAb
Recombinant
了解详情

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(1 Publication)

Rabbit Recombinant Monoclonal OLF1 antibody. Carrier free. Suitable for WB and reacts with Human, Rat, Mouse samples. Cited in 1 publication.

查看别名

OLF1, OR5I1, Olfactory receptor 5I1, Olfactory receptor OR11-159, Olfactory receptor-like protein OLF1

2 Images

Supplier Data

Western blot - Anti-OLF1 antibody [EPR15972] - BSA and Azide free (AB251035)

This data was developed using ab190792, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-OLF1 antibody [EPR15972] (<a href='/products/primary-antibodies/olf1-antibody-epr15972-ab190792'>ab190792</a>) at 1/10000 dilution

Lane 1:

Human fetal brain lysate at 20 µg

Lane 2:

Human fetal heart lysate at 20 µg

Lane 3:

HepG2 cell lysate at 20 µg

Lane 4:

Jurkat cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 36 kDa

Observed band size: 36 kDa

false

Supplier Data

Western blot - Anti-OLF1 antibody [EPR15972] - BSA and Azide free (AB251035)

This data was developed using ab190792, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-OLF1 antibody [EPR15972] (<a href='/products/primary-antibodies/olf1-antibody-epr15972-ab190792'>ab190792</a>) at 1/1000 dilution

Lane 1:

Mouse brain lysate at 10 µg

Lane 2:

Mouse heart lysate at 10 µg

Lane 3:

Mouse kidney lysate at 10 µg

Lane 4:

Mouse spleen lysate at 10 µg

Lane 5:

Rat brain lysate at 10 µg

Lane 6:

Rat heart lysate at 10 µg

Lane 7:

Rat kidney lysate at 10 µg

Lane 8:

Rat spleen lysate at 10 µg

Lane 9:

C6 cell lysate at 10 µg

Lane 10:

Raw264.7 cell lysate at 10 µg

Lane 11:

PC12 cell lysate at 10 µg

Lane 12:

NIH3T3 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 36 kDa

Observed band size: 36 kDa

false

不同偶联物与剂型 (1)

Unconjugated

Anti-OLF1 antibody [EPR15972]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR15972

亚型

IgG

不含载体蛋白

Yes

反应种属

Mouse, Rat, Human

应用

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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产品详情

ab251035 is the carrier-free version of ab190792.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式

Liquid

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

储存信息

Do Not Freeze

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

OLF1 also known as OLF-1-like protein or Olfactory neuron specific factor 1 functions mainly as a transcription factor. It weighs approximately 34 kDa and is primarily expressed in neurons and tissues related to the olfactory system. OLF1 binds specific DNA sequences to regulate the transcription of genes involved in olfactory receptor expression. It plays a critical role in the development and function of the olfactory system by influencing gene networks specific to olfactory receptors.

Biological function summary

OLF1 impacts the expression of olfactory receptor genes by interacting with other transcription factors within the nucleus. It functions as part of larger transcriptional complexes to modulate olfactory receptor gene expression. Through these activities OLF1 contributes significantly to the regulation of sensory perception in mammals. Its precise role in olfactory development and sensory neuron identity highlights its importance in neural biology.

Pathways

OLF1 is integrated into the olfactory signaling pathway. It cooperates with proteins like O/E-1 and O/E-3 to regulate olfactory receptor gene clusters. This regulation is important for odor detection and signal transduction in the olfactory pathway. The olfactory signaling pathway involves multiple stages including ligand binding and signal transduction in which OLF1 contributes through its transcriptional regulatory functions.

Alterations in OLF1 activity have been linked to anosmia a condition characterized by the loss of smell. Researchers also explore its involvement in neurodegenerative disorders where olfactory deficits are early symptoms such as Parkinson’s disease. Examining OLF1 interactions with proteins such as alpha-synuclein provides insights into its potential role in the pathogenesis of these conditions.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Odorant receptor.

See full target information OR5I1

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 21:454-462 PubMed31746378

2019

Blueberry anthocyanin‑enriched extract ameliorates transverse aortic constriction‑induced myocardial dysfunction via the DDAH1/ADMA/NO signaling pathway in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Weiqing Hu,Wenyue Wang,Qing Ma,Tao Liu,Jiefeng Zhang,Jicun Zhang

View all publications

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详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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重组Anti-OLF1抗体[EPR15972] - BSA and Azide free