重组Anti-OGT / O-Linked N-Acetylglucosamine Transferase抗体[EPR12713] (ab177941)
![Flow Cytometry (Intracellular) - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab177941)](https://www.abcam.cn/ps/products/177/ab177941/Images/ab177941-506131-anti-ogt-o-linked-n-acetylglucosamine-transferase-antibody-epr12713-flowcytometry-k562-human.jpg "Flow Cytometry (Intracellular) - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab177941)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12713] to OGT / O-Linked N-Acetylglucosamine Transferase
- Suitable for: Flow Cyt (Intra), WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
-
产品名称
Anti-OGT / O-Linked N-Acetylglucosamine Transferase抗体[EPR12713]
参阅全部 OGT / O-Linked N-Acetylglucosamine Transferase 一抗 -
描述
兔单克隆抗体[EPR12713] to OGT / O-Linked N-Acetylglucosamine Transferase -
宿主
Rabbit -
特异性
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
-
经测试应用
适用于: Flow Cyt (Intra), WB, ICC/IF, IHC-Pmore details
不适用于: IP -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
阳性对照
- WB: A549, HeLa, and 293T cell lysates, Mouse heart and pancreas, Rat heart. IHC-P: human breast carcinoma tissue and lung carcinoma tissue. Flow Cyt (intra): K562 cells. ICC/IF: A549 cells.
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR12713 -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
- Alexa Fluor® 647 Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab198532)
- Alexa Fluor® 555 Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab215809)
- Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] - BSA and Azide free (ab236014)
- Alexa Fluor® 568 Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab312586)
- Alexa Fluor® 750 Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab321209)
-
Isotype control
-
Positive Controls
-
Recombinant Protein
-
Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab177941于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB | (2) |
1/1000 - 1/5000. Detects a band of approximately 110 kDa (predicted molecular weight: 116 kDa).
|
| ICC/IF |
1/50 - 1/100.
|
|
| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. |
| 说明 |
|---|
|
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/1000 - 1/5000. Detects a band of approximately 110 kDa (predicted molecular weight: 116 kDa). |
|
ICC/IF
1/50 - 1/100. |
|
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. |
靶标
-
功能
Addition of nucleotide-activated sugars directly onto the polypeptide through O-glycosidic linkage with the hydroxyl of serine or threonine. Mediates the O-glycosylation of MLL5 and HCFC1. Promotes proteolytic maturation of HCFC1. -
组织特异性
Highly expressed in pancreas and to a lesser extent in skeletal muscle, heart, brain and placenta. Present in trace amounts in lung and liver. -
通路
Protein modification; protein glycosylation. -
序列相似性
Belongs to the O-GlcNAc transferase family.
Contains 13 TPR repeats. -
结构域
The TPR repeat domain mediates recognition of protein substrates. -
翻译后修饰
Ubiquitinated, leading to its proteasomal degradation. -
细胞定位
Cytoplasm. Nucleus. Mostly in the nucleus. - Information by UniProt
-
数据库链接
- Entrez Gene: 8473 Human
- Entrez Gene: 108155 Mouse
- Entrez Gene: 26295 Rat
- Omim: 300255 Human
- SwissProt: O15294 Human
- SwissProt: Q8CGY8 Mouse
- SwissProt: P56558 Rat
- Unigene: 405410 Human
see all -
别名
- FLJ23071 antibody
- GlcNAc transferase antibody
- HRNT1 antibody
see all
图片
-
Flow Cytometry (Intracellular) - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab177941)
Intracellular Flow Cytometry analysis of K-562 (Human chronic myelogenous leukemia lymphoblast) cells labeling OGT / O-Linked N-Acetylglucosamine Transferase with purified ab177941 at 1:40 dilution (10 μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as a isotype control. Cell without incubation with primary antibody and secondary antibody (Blue) were used as unlabeled control.
-
![Immunocytochemistry/ Immunofluorescence - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab177941)](https://www.abcam.cn/ps/products/177/ab177941/Images/ab177941-506130-anti-ogt-o-linked-n-acetylglucosamine-transferase-antibody-epr12713-immunocytochemistry-a549-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab177941)Immunocytochemistry/ Immunofluorescence analysis of A549 (Human lung carcinoma epithelial cell) cells labeling OGT / O-Linked N-Acetylglucosamine Transferase using ab177941. The cells were fixed with 4% paraformaldehyde then permeabilized with 0.1% Triton X-100. The cells were then incubated with ab177941 at 1:100 dilution followed by a further incubation with a Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI. Cells were counterstained using ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1:200 dilution (shown in red). Secondary antibody only control: PBS instead of the primary antibody.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab177941)](https://www.abcam.cn/ps/products/177/ab177941/Images/ab177941-506129-anti-ogt-o-linked-n-acetylglucosamine-transferase-antibody-epr12713-immunohistochemistry-lung-carcinoma-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab177941)Immunohistochemical analysis of Paraffin-embedded sections human lung carcinoma tissue labelling OGT / O-Linked N-Acetylglucosamine Transferase with ab177941 at 1:2000 dilution (0.2 μg/ml), followed by a ready to use secondary Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Staining on human lung carcinoma tissue is observed. Counter stained with Haematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0).
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab177941)](https://www.abcam.cn/ps/products/177/ab177941/Images/ab177941-506128-anti-ogt-o-linked-n-acetylglucosamine-transferase-antibody-epr12713-immunohistochemistry-breast-carcinoma-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab177941)Immunohistochemical analysis of Paraffin-embedded sections human breast carcinoma tissue labelling OGT / O-Linked N-Acetylglucosamine Transferase with ab177941 at 1:2000 dilution (0.2 μg/ml), followed by a ready to use secondary Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Staining on human breast carcinoma tissue is observed. Counter stained with Haematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0).
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
-
![Western blot - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab177941)](https://www.abcam.cn/ps/products/177/ab177941/Images/ab177941-506127-anti-ogt-o-linked-n-acetylglucosamine-transferase-antibody-epr12713-westernblot-human-mouse-rat.jpg)
Western blot - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab177941)All lanes : Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab177941) at 1/10000 dilution
Lane 1 : A549 (Human lung carcinoma epithelial cell) whole cell lysate
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 3 : 293T (Human embryonic kidney epithelial cell) whole cell lysate
Lane 4 : Mouse heart
Lane 5 : Mouse pancreas
Lane 6 : Rat heart
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 116 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
ab181602 was used as GAPDH loading control.
-
![Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab177941)](https://www.abcam.cn/ps/products/177/ab177941/Images/ab177941-6-benefits-of-recombinant-antibodies.png)
Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab177941)
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (26)
ab177941 被引用在 26 文献中.
- Zhang C et al. CircVPRBP inhibits nodal metastasis of cervical cancer by impeding RACK1 O-GlcNAcylation and stability. Oncogene 42:793-807 (2023). PubMed: 36658304
- Lu P et al. Cryo-EM structure of human O-GlcNAcylation enzyme pair OGT-OGA complex. Nat Commun 14:6952 (2023). PubMed: 37907462
- Mathew MP et al. Nutrient-responsive O-GlcNAcylation dynamically modulates the secretion of glycan-binding protein galectin 3. J Biol Chem 298:101743 (2022). PubMed: 35183508
- Li C et al. Hypertonic stress modulates eNOS function through O-GlcNAc modification at Thr-866. Sci Rep 11:11272 (2021). PubMed: 34050207
- Wu J et al. Melatonin reduces proliferation and promotes apoptosis of bladder cancer cells by suppressing O-GlcNAcylation of cyclin-dependent-like kinase 5. J Pineal Res 71:e12765 (2021). PubMed: 34487576