Anti-OGT / O-Linked N-Acetylglucosamine Transferase抗体(ab96718)
Key features and details
- Rabbit polyclonal to OGT / O-Linked N-Acetylglucosamine Transferase
- Suitable for: ICC/IF, WB, IHC-P, IP
- Reacts with: Mouse, Rat, Human, Zebrafish
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-OGT / O-Linked N-Acetylglucosamine Transferase抗体
参阅全部 OGT / O-Linked N-Acetylglucosamine Transferase 一抗 -
描述
兔多克隆抗体to OGT / O-Linked N-Acetylglucosamine Transferase -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WB, IHC-P, IPmore details -
种属反应性
与反应: Mouse, Rat, Human, Zebrafish
预测可用于: Cow, Pig
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免疫原
Recombinant fragment corresponding to Human OGT/ O-Linked N-Acetylglucosamine Transferase aa 213-462.
Database link: O15294 -
阳性对照
- WB: HeLa, PC-12 cells. Mouse brain tissue extracts. ICC/IF: MCF7, DU145, HeLa cells. IHC-P: Cal27 Xenograft. Rat hind brain. IP: A431 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab96718于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use at an assay dependent concentration.
|
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| WB | (8) |
1/500 - 1/3000. Predicted molecular weight: 117 kDa.
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| IHC-P | (1) |
1/100 - 1/1000.
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| IP |
1/100 - 1/500.
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| 说明 |
|---|
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ICC/IF
Use at an assay dependent concentration. |
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WB
1/500 - 1/3000. Predicted molecular weight: 117 kDa. |
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IHC-P
1/100 - 1/1000. |
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IP
1/100 - 1/500. |
靶标
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功能
Addition of nucleotide-activated sugars directly onto the polypeptide through O-glycosidic linkage with the hydroxyl of serine or threonine. Mediates the O-glycosylation of MLL5 and HCFC1. Promotes proteolytic maturation of HCFC1. -
组织特异性
Highly expressed in pancreas and to a lesser extent in skeletal muscle, heart, brain and placenta. Present in trace amounts in lung and liver. -
通路
Protein modification; protein glycosylation. -
序列相似性
Belongs to the O-GlcNAc transferase family.
Contains 13 TPR repeats. -
结构域
The TPR repeat domain mediates recognition of protein substrates. -
翻译后修饰
Ubiquitinated, leading to its proteasomal degradation. -
细胞定位
Cytoplasm. Nucleus. Mostly in the nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 532053 Cow
- Entrez Gene: 8473 Human
- Entrez Gene: 108155 Mouse
- Entrez Gene: 664652 Pig
- Entrez Gene: 26295 Rat
- Entrez Gene: 337685 Zebrafish
- Omim: 300255 Human
- SwissProt: O15294 Human
see all -
别名
- FLJ23071 antibody
- GlcNAc transferase antibody
- HRNT1 antibody
see all
图片
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Western blot - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab96718)Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab96718) at 1/1000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 117 kDa
7.5% SDS PAGE -
Western blot - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab96718)All lanes : Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab96718) at 1 µg/ml
Lane 1 : Marker
Lane 2 : Zebrafish brain homogenate at 20 µg
Lane 3 : Zebrafish heart homogenate at 20 µg
Lane 4 : Zebrafish liver homogenate at 20 µg
Lane 5 : Zebrafish skeletal muscle homogenate at 20 µg
Lane 6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg
Secondary
All lanes : Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 117 kDa
Observed band size: 117 kDa
Exposure time: 5 minutes -
Immunocytochemistry/ Immunofluorescence - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab96718)HeLa cells stained for O-GlcNAc (green) using ab96718 at 1/200 dilution in ICC/IF.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab96718)Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft, using ab96718 at 1/100 dilution. -
Immunoprecipitation - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab96718)Immunoprecipitation analysis of OGT/O-linked N-Acetylglucosamine Transferase protein from A431 whole cell extracts using ab96718 (5µg). Western blot analysis was performed using ab96718 and an Easyblot anti-rabbit IgG was used as the secondary antibody.
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Immunocytochemistry/ Immunofluorescence - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab96718)Ab96718 staining OGT / O-Linked N-Acetylglucosamine Transferase in MCF7 cells by ICC/IF (Immunocytochemistry/Immunofluorescence). MFC7 cells were fixed with paraformaldehyde at room temperature for 15 minutes. Samples were incubated with primary antibody at a 1:500 dilution.
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Western blot - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab96718)Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab96718) at 1/1000 dilution + PC-12 whole cell extract at 30 µg
Predicted band size: 117 kDa5% SDS-PAGE
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Immunocytochemistry/ Immunofluorescence - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab96718)ICC/IF image of ab96718 stained DU145 cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab96718, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab96718)Paraffin embedded rat hind brain tissue stained for O-GlcNAc using ab96718 at 1/500 in immunohistochemical analysis.
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Western blot - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab96718)Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab96718) at 1/2000 dilution + Mouse brain tissue extract at 50 µg
Predicted band size: 117 kDa7.5% SDS-PAGE
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (36)
ab96718 被引用在 36 文献中.
- Yu F et al. Dynamic O-GlcNAcylation coordinates ferritinophagy and mitophagy to activate ferroptosis. Cell Discov 8:40 (2022). PubMed: 35504898
- Lee HW et al. Targeted Inhibition of O-Linked β-N-Acetylglucosamine Transferase as a Promising Therapeutic Strategy to Restore Chemosensitivity and Attenuate Aggressive Tumor Traits in Chemoresistant Urothelial Carcinoma of the Bladder. Biomedicines 10:N/A (2022). PubMed: 35625898
- Wang TF et al. Disruption of O-GlcNAcylation Homeostasis Induced Ovarian Granulosa Cell Injury in Bovine. Int J Mol Sci 23:N/A (2022). PubMed: 35887161
- Xiong X et al. Intestinal Epithelial STAT6 Activation Rescues the Defective Anti-Helminth Responses Caused by Ogt Deletion. Int J Mol Sci 23:N/A (2022). PubMed: 36232438
- Cao Y et al. O-GlcNAcylation of SIRT1 Protects against Cold Stress-Induced Skeletal Muscle Damage via Amelioration of Mitochondrial Homeostasis. Int J Mol Sci 23:N/A (2022). PubMed: 36498847