Anti-OGT / O-Linked N-Acetylglucosamine Transferase抗体(ab59135)
Key features and details
- Goat polyclonal to OGT / O-Linked N-Acetylglucosamine Transferase
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgG
概述
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产品名称
Anti-OGT / O-Linked N-Acetylglucosamine Transferase抗体
参阅全部 OGT / O-Linked N-Acetylglucosamine Transferase 一抗 -
描述
山羊多克隆抗体to OGT / O-Linked N-Acetylglucosamine Transferase -
宿主
Goat -
特异性
ab59135 is expected to recognize both reported isoforms of O-Linked N-Acetylglucosamine -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Rat
预测可用于: Mouse, Cow, Dog, Pig, Xenopus laevis, Zebrafish
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免疫原
Synthetic peptide:
C-YEHPKDLKLSDGR
by a Cysteine residue linker, corresponding to amino acids 543-555 of Human O-Linked N-Acetylglucosamine -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab59135于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 0.05 - 0.2 µg/ml. Detects a band of approximately 110 kDa.
A 1 hour primary incubation is recommended for this product. |
| 说明 |
|---|
|
WB
Use a concentration of 0.05 - 0.2 µg/ml. Detects a band of approximately 110 kDa. A 1 hour primary incubation is recommended for this product. |
靶标
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功能
Addition of nucleotide-activated sugars directly onto the polypeptide through O-glycosidic linkage with the hydroxyl of serine or threonine. Mediates the O-glycosylation of MLL5 and HCFC1. Promotes proteolytic maturation of HCFC1. -
组织特异性
Highly expressed in pancreas and to a lesser extent in skeletal muscle, heart, brain and placenta. Present in trace amounts in lung and liver. -
通路
Protein modification; protein glycosylation. -
序列相似性
Belongs to the O-GlcNAc transferase family.
Contains 13 TPR repeats. -
结构域
The TPR repeat domain mediates recognition of protein substrates. -
翻译后修饰
Ubiquitinated, leading to its proteasomal degradation. -
细胞定位
Cytoplasm. Nucleus. Mostly in the nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 532053 Cow
- Entrez Gene: 108155 Mouse
- Entrez Gene: 664652 Pig
- Entrez Gene: 26295 Rat
- Entrez Gene: 337685 Zebrafish
- SwissProt: Q8CGY8 Mouse
- SwissProt: Q27HV0 Pig
- SwissProt: P56558 Rat
see all -
别名
- FLJ23071 antibody
- GlcNAc transferase antibody
- HRNT1 antibody
see all
图片
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Western blot - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab59135)Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab59135) at 0.05 µg/ml + Rat pancreas lysate in RIPA buffer at 35 µg
Observed band size: 110 kDa why is the actual band size different from the predicted?
Additional bands at: 60 kDa. We are unsure as to the identity of these extra bands.An additional band of unknown identity was also consistently observed at 60kDa. This band was successfully blocked by incubation with the immunizing peptide. Primary incubation was 1 hour. Detected by chemiluminescence.
Preliminary testing was unsuccessful on Mouse Brain for this particular batch.
数据表及文件
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Datasheet download
文献 (3)
ab59135 被引用在 3 文献中.
- Moura CS et al. Bioactivity of food peptides: biological response of rats to bovine milk whey peptides following acute exercise. Food Nutr Res 61:1290740 (2017). WB ; Rat . PubMed: 28326005
- Moura CS et al. Whey protein hydrolysate enhances HSP90 but does not alter HSP60 and HSP25 in skeletal muscle of rats. PLoS One 9:e83437 (2014). WB ; Rat . PubMed: 24465380
- Andrés-Bergós J et al. The increase in o-linked N-acetylglucosamine protein modification stimulates chondrogenic differentiation both in vitro and in vivo. J Biol Chem 287:33615-28 (2012). WB . PubMed: 22859309
