Anti-Oct6抗体(ab272925)
Key features and details
- Rabbit polyclonal to Oct6
- Suitable for: IHC-Fr, WB, ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-Oct6抗体
参阅全部 Oct6 一抗 -
描述
兔多克隆抗体to Oct6 -
宿主
Rabbit -
经测试应用
适用于: IHC-Fr, WB, ICC/IF, IHC-Pmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Recombinant fragment within Mouse Oct6. The exact sequence is proprietary. Center region.
Database link: P21952 -
阳性对照
- WB: Mouse fetal brain tissue extract. ICC/IF: A549 cells. IHC-P: Mouse E10.5 embryo tissue. IHC-Fr: Mouse cerebral cortex tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab272925于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-Fr |
1/100 - 1/1000.
|
|
| WB |
1/500 - 1/3000.
|
|
| ICC/IF |
1/100 - 1/1000.
|
|
| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
IHC-Fr
1/100 - 1/1000. |
|
WB
1/500 - 1/3000. |
|
ICC/IF
1/100 - 1/1000. |
|
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Thought to be involved in early embryogenesis and neurogenesis. -
组织特异性
Expressed in embryonal stem cells and in the developing brain. -
序列相似性
Belongs to the POU transcription factor family. Class-3 subfamily.
Contains 1 homeobox DNA-binding domain.
Contains 1 POU-specific domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 5453 Human
- Entrez Gene: 18991 Mouse
- Omim: 602479 Human
- SwissProt: Q03052 Human
- SwissProt: P21952 Mouse
- Unigene: 1837 Human
- Unigene: 297371 Mouse
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别名
- class 3 antibody
- Oct-6 antibody
- OCT6 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct6 antibody (ab272925)
Paraffin-embedded mouse E10.5 embryo tissue stained for Oct6 using ab272925 at 1/500 dilution (green) in immunohistochemical analysis. Antigen retrieval using citrate buffer, pH 6, 15 minutes. Red: SOX2. Blue: DAPI.
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Immunohistochemistry (Frozen sections) - Anti-Oct6 antibody (ab272925)Frozen mouse cerebral cortex tissue stained for Oct6 using ab272925 at 1/250 dilution (green) in immunohistochemical analysis. Blue: DAPI.
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Western blot - Anti-Oct6 antibody (ab272925)Anti-Oct6 antibody (ab272925) at 1/500 dilution + Mouse fetal brain tissue extract at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody
Developed using the ECL technique.10% SDS-PAGE
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Immunocytochemistry/ Immunofluorescence - Anti-Oct6 antibody (ab272925)4% PFA-fixed A549 (Human lung carcinoma cell line) cells stained for Oct6 using ab272925 at 1/500 dilution in ICC/IF (green). Red: Phalloidin.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct6 antibody (ab272925)Paraffin-embedded mouse E10.5 embryo tissue stained for Oct6 using ab272925 at 1/250 dilution (green) in immunohistochemical analysis. Antigen retrieval using citrate buffer, pH 6, 15 minutes.
Red: Beta tubulin.
Blue: DAPI.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab272925 被引用在 5 文献中.
- Ermakova VV et al. Regulatory Elements Outside Established Pou5f1 Gene Boundaries Are Required for Multilineage Differentiation of Embryonic Stem Cells. Int J Mol Sci 24:N/A (2023). PubMed: 37895112
- Conforti P et al. In vitro-derived medium spiny neurons recapitulate human striatal development and complexity at single-cell resolution. Cell Rep Methods 2:100367 (2022). PubMed: 36590694
- Kastriti ME et al. Schwann cell precursors represent a neural crest-like state with biased multipotency. EMBO J 41:e108780 (2022). PubMed: 35815410
- Bocchi VD et al. The coding and long noncoding single-cell atlas of the developing human fetal striatum. Science 372:N/A (2021). PubMed: 33958447
- Ciarpella F et al. Murine cerebral organoids develop network of functional neurons and hippocampal brain region identity. iScience 24:103438 (2021). PubMed: 34901791