重组Anti-Oct-2抗体[EP543Y] (ab33919)
![Western blot - Anti-Oct-2 antibody [EP543Y] (ab33919)](https://www.abcam.cn/ps/products/33/ab33919/Images/ab33919-553682-anti-oct-2-antibody-ep543Y-western-blot-human-raji-daudi.jpg "Western blot - Anti-Oct-2 antibody [EP543Y] (ab33919)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP543Y] to Oct-2
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Oct-2抗体[EP543Y]
参阅全部 Oct-2 一抗 -
描述
兔单克隆抗体[EP543Y] to Oct-2 -
宿主
Rabbit -
特异性
This antibody may detect the isoform 2.
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经测试应用
适用于: WBmore details
不适用于: ChIP or Flow Cyt -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Raji and Daudi whole cell lysates.
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常规说明
This product has switched from a hybridoma to recombinant production method on 13th September 2024.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP543Y -
同种型
IgG -
研究领域
相关产品
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab33919于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/10000. Predicted molecular weight: 51 kDa.
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| 说明 |
|---|
|
WB
1/10000. Predicted molecular weight: 51 kDa. |
靶标
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功能
Transcription factor that specifically binds to the octamer motif (5'-ATTTGCAT-3'). Regulates transcription in a number of tissues in addition to activating immunoglobulin gene expression. Modulates transcription transactivation by NR3C1, AR and PGR. Isoform 5 activates the U2 small nuclear RNA (snRNA) promoter. -
组织特异性
Isoform 3 is B-cell specific. Isoform 5 is expressed in B-cells and the immunoglobulin-expressing T-cell line MOLT-4, but not in the T-cell line BW5147. -
序列相似性
Belongs to the POU transcription factor family. Class-2 subfamily.
Contains 1 homeobox DNA-binding domain.
Contains 1 POU-specific domain. -
细胞定位
Cytoplasm. Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 5452 Human
- Omim: 164176 Human
- SwissProt: P09086 Human
- Unigene: 147381 Human
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别名
- class 2 antibody
- Lymphoid restricted immunoglobulin octamer binding protein antibody
- Lymphoid-restricted immunoglobulin octamer-binding protein NF-A2 antibody
see all
图片
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Western blot - Anti-Oct-2 antibody [EP543Y] (ab33919)All lanes : Anti-Oct-2 antibody [EP543Y] (ab33919) at 1/10000 dilution
Lane 1 : Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate
Lane 2 : Daudi (Human Burkitt's lymphoma lymphoblast) whole cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 51 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Exposure time: 10 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab33919 被引用在 3 文献中.
- Wei L et al. Functional role of PLCE1 intronic insertion variant associated with susceptibility to esophageal squamous cell carcinoma. Carcinogenesis 39:191-201 (2018). PubMed: 29106514
- Oguri T et al. Organic cation transporter 6 directly confers resistance to anticancer platinum drugs. Biomed Rep 5:639-643 (2016). WB ; Human . PubMed: 27882231
- Winiarska M et al. Prenyltransferases Regulate CD20 Protein Levels and Influence Anti-CD20 Monoclonal Antibody-mediated Activation of Complement-dependent Cytotoxicity. J Biol Chem 287:31983-93 (2012). ChIP . PubMed: 22843692