AB181039

Anti-OCRL抗体[EP10256]

Anti-OCRL antibody [EP10256]

RabMAb
KO Validated
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(1 Publication)

Rabbit Monoclonal OCRL antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

查看别名

OCRL1, OCRL, Inositol polyphosphate 5-phosphatase OCRL, Inositol polyphosphate 5-phosphatase OCRL-1, Lowe oculocerebrorenal syndrome protein, OCRL-1

3 Images

Flow Cytometry (Intracellular) - Anti-OCRL antibody [EP10256] (AB181039)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-OCRL antibody [EP10256] (AB181039)

Intracellular flow cytometric analysis of permeabilized SH-SY5Y cells using ab181039 at a 1/10 dilution (red) or a rabbit IgG (negative) (green).

Supplier Data

Western blot - Anti-OCRL antibody [EP10256] (AB181039)

All lanes:

Western blot - Anti-OCRL antibody [EP10256] (ab181039) at 1000 µg

Lane 1:

293T cell lysates at 10 µg

Lane 2:

SH-SY5Y cell lysates at 10 µg

Lane 3:

HeLa cell lysates at 10 µg

Lane 4:

JAR cell lysates at 10 µg

Predicted band size: 104 kDa

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Lab

Western blot - Anti-OCRL antibody [EP10256] (AB181039)

Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : INPP5F knockout HAP1 cell lysate (20 μg)
Lane 3 : SH-SY5Y cell lysate (20 μg)
Lane 4 : HeLa cell lysate (20 μg)
Lanes 1 - 4 : Merged signal (red and green). Green - ab181039 observed at 105 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab181039 was shown to recognize INPP5F when INPP5F knockout samples were used, along with additional cross-reactive bands. Wild-type and INPP5F knockout samples were subjected to SDS-PAGE. ab181039 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-OCRL antibody [EP10256] (ab181039)

Predicted band size: 104 kDa

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不同偶联物与剂型 (1)

Carrier free

Anti-OCRL antibody [EP10256] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EP10256

亚型

IgG

不含载体蛋白

反应种属

Human

应用

WB, Flow Cyt (Intra)

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." } } }

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产品详情

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

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性能和储存信息

形式

Liquid

纯度

Tissue culture supernatant

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The OCRL protein also known as oculocerebrorenal syndrome of Lowe protein is a phosphatidylinositol 45-bisphosphate 5-phosphatase involved in cellular membrane trafficking. It has a molecular mass of approximately 105 kDa. Localization of OCRL occurs in several cellular compartments including the endosomes and the Golgi apparatus. This protein is widely expressed in tissues such as the brain kidney and eyes. Scientists often study OCRL to better understand its role in cellular functions and associated disorders.

Biological function summary

OCRL participates in the regulation of phosphoinositide metabolism by dephosphorylating phosphatidylinositol 45-bisphosphate an important signaling lipid. OCRL is a member of the inositol polyphosphate 5-phosphatase family and associates with clathrin-mediated endocytosis complexes. The protein ensures proper vesicular trafficking and maintenance of cell membrane dynamics impacting processes like endocytosis and ciliogenesis. Researchers focus on understanding OCRL's interactions and dynamics to comprehend its contribution to these processes.

Pathways

OCRL plays a significant role in the phosphoinositide signaling pathway and is integral to cellular trafficking pathways. In the phosphoinositide signaling pathway OCRL modulates the availability of phosphatidylinositol 45-bisphosphate interacting with proteins like APPL1 and Rab GTPases. In cellular trafficking pathways OCRL coordinates with proteins such as clathrin and dynamin to regulate endocytosis and endosomal function. Understanding OCRL's placement within these pathways provides insight into its impact on cellular homeostasis.

OCRL mutations are linked to Lowe syndrome and Dent disease both of which involve renal and ocular complications. Lowe syndrome characterized by congenital cataracts intellectual disabilities and renal Fanconi syndrome directly associates with OCRL dysfunction. The OCRL protein interacts with clathrin and Rab GTPases contributing to the pathogenesis of these disorders by disrupting normal vesicle trafficking processes. Research on OCRL-related mechanisms in these diseases seeks to unravel potential therapeutic targets and interventions.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Catalyzes the hydrolysis of the 5-position phosphate of phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) and phosphatidylinositol-3,4,5-bisphosphate (PtdIns(3,4,5)P3), with the greatest catalytic activity towards PtdIns(4,5)P2 (PubMed : 10764818, PubMed : 15474001, PubMed : 7761412, PubMed : 9430698). Able also to hydrolyze the 5-phosphate of inositol 1,4,5-trisphosphate and of inositol 1,3,4,5-tetrakisphosphate (PubMed : 25869668, PubMed : 7761412). Regulates traffic in the endosomal pathway by regulating the specific pool of phosphatidylinositol 4,5-bisphosphate that is associated with endosomes (PubMed : 21971085). Involved in primary cilia assembly (PubMed : 22228094, PubMed : 22543976). Acts as a regulator of phagocytosis, hydrolyzing PtdIns(4,5)P2 to promote phagosome closure, through attenuation of PI3K signaling (PubMed : 22072788).

See full target information OCRL

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of cell biology 216:3745-3765 PubMed28923975

2017

Control of actin polymerization via the coincidence of phosphoinositides and high membrane curvature.

Applications

Unspecified application

Species

Unspecified reactive species

Frederic Daste,Astrid Walrant,Mikkel R Holst,Jonathan R Gadsby,Julia Mason,Ji-Eun Lee,Daniel Brook,Marcel Mettlen,Elin Larsson,Steven F Lee,Richard Lundmark,Jennifer L Gallop

View all publications

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Anti-OCRL抗体[EP10256]