Anti-OBSL1抗体(ab224588)
Key features and details
- Rabbit polyclonal to OBSL1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-OBSL1抗体
参阅全部 OBSL1 一抗 -
描述
兔多克隆抗体to OBSL1 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein corresponding to Human OBSL1 aa 1-61.
Database link: Q6P710 -
阳性对照
- WB: HepG2, MCF7 and A549 cell lysate. IHC: Human placenta tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
纯度
Protein G purified -
克隆
多克隆 -
同种型
IgG
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab224588于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/5000. Detects a band of approximately 207 kDa (predicted molecular weight: 207 kDa).
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| IHC-P |
1/20 - 1/200.
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| 说明 |
|---|
|
WB
1/500 - 1/5000. Detects a band of approximately 207 kDa (predicted molecular weight: 207 kDa). |
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IHC-P
1/20 - 1/200. |
靶标
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组织特异性
Widely expressed, with predominant levels found in the heart. -
疾病相关
Defects in OBSL1 are the cause of 3M syndrome type 2 (3M2) [MIM:612921]. An autosomal recessive disorder characterized by severe pre- and postnatal growth retardation, facial dysmorphism, large head circumference, and normal intelligence and endocrine function. Skeletal changes include long slender tubular bones and tall vertebral bodies. -
序列相似性
Contains 1 fibronectin type-III domain.
Contains 11 Ig-like (immunoglobulin-like) domains. - Information by UniProt
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数据库链接
- Entrez Gene: 23363 Human
- Omim: 610991 Human
- SwissProt: O75147 Human
- Unigene: 526594 Human
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别名
- KIAA0657 antibody
- Obscurin-like protein 1 antibody
- OBSL1 antibody
- OBSL1_HUMAN antibody
图片
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Western blot - Anti-OBSL1 antibody (ab224588)All lanes : Anti-OBSL1 antibody (ab224588) at 1/500 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Lane 2 : MCF7 (human breast adenocarcinoma cell line) cell lysate
Lane 3 : A549 (human lung carcinoma cell line) cell lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 207 kDa
Observed band size: 207 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OBSL1 antibody (ab224588)Paraffin-embedded human placenta tissue stained for OBSL1 using ab224588 at 1/100 dilution in immunohistochemical analysis.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab224588 尚未被引用在任何文献中。