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Anti-OATP1B1 + OATP1B3抗体[MDQ] (ab15442)

Reviews (2)Q&A (4)References (3)
Immunocytochemistry/ Immunofluorescence - Anti-OATP1B1 + OATP1B3 antibody [MDQ] (ab15442)

    Key features and details

    • Mouse monoclonal [MDQ] to OATP1B1 + SLCO1B3/OATP1B3
    • Suitable for: ICC/IF
    • Reacts with: Human
    • Isotype: IgG1

    概述

    • 产品名称

      Anti-OATP1B1 + OATP1B3抗体[MDQ]

    • 描述

      小鼠单克隆抗体[MDQ] to OATP1B1 + SLCO1B3/OATP1B3

    • 宿主

      Mouse

    • 特异性

      ab15442 will also cross react with OATP8.

    • 经测试应用

      适用于: ICC/IFmore details

    • 种属反应性

      与反应: Human
      不与反应: Mouse, Rat, Dog

    • 免疫原

      Synthetic peptide corresponding to Human OATP1B1 aa 1-100.
      Database link: Q9Y6L6

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • 阳性对照

      • Human liver tissue slides.

    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab15442于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    ICC/IF
    1/20.
    说明
    ICC/IF
    1/20.

    靶标

    图片

    • Immunocytochemistry/ Immunofluorescence - Anti-OATP1B1 + OATP1B3 antibody [MDQ] (ab15442)
      Immunocytochemistry/ Immunofluorescence - Anti-OATP1B1 + OATP1B3 antibody [MDQ] (ab15442)

      Immunocytochemistry/Immunofluorescence analysis of HepG2 cells labeling OATP1B1 + OATP1B3 (green) with ab15542 at 1/20. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control.

    文献 (3)

    发表研究结果有使用 ab15442？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab15442 被引用在 3 文献中.

    • Imada S  et al. Central bisectionectomy for hepatocellular carcinoma in a patient with indocyanine green excretory defect associated with reduced expression of the liver transporter. Surg Case Rep 2:89 (2016). PubMed: 27589984
    • Peng JF  et al. Role of miR-511 in the Regulation of OATP1B1 Expression by Free Fatty Acid. Biomol Ther (Seoul) 23:400-6 (2015). WB . PubMed: 26336578
    • Sticova E  et al. Down-regulation of OATP1B proteins correlates with hyperbilirubinemia in advanced cholestasis. Int J Clin Exp Pathol 8:5252-62 (2015). PubMed: 26191226

    客户评价及客户问答

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    1-6 of 6 Abreviews or Q&A

    Western blot abreview for Anti-OATP2 antibody [MDQ]

     Poor
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (human HEK293 cells over-expressing)
    Loading amount
    150 µg
    Specification
    human HEK293 cells over-expressing
    Gel Running Conditions
    Reduced Denaturing (denature at 70C for 10mins, 7.5% gel)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Sep 08 2011

    Western blot abreview for Anti-OATP1B1 antibody [MDQ]

     Average
    Abreviews
    Application
    Western blot
    Sample
    Human Cell lysate - other (transiently transfected HeLa cells)
    Loading amount
    100000 cells
    Specification
    transiently transfected HeLa cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Dec 19 2006

    Question

    I’ve looked on your website but havent found MSDSs for these. Could I get electronic copies of the following?

    Read More
    Answer



    Any products that require an MSDS form will have a link on the product's online datasheet to the data. It will be a PDF document link labeled 'SDS'. Please see attached image for example of what to look for on the datasheet. If no 'SDS' link is available than the product does not contain any components that require an SDS.

    Read More

    Question

    BATCH NUMBER -- NOT SPECIFIED -- ORDER NUMBER 173888 DESCRIPTION OF THE PROBLEM no band. but other lot well work. SAMPLE human, HEK293-OATP1B3 transfected cells. PRIMARY ANTIBODY Mouse monoclonal [MDQ] to OATP2 (ab15442), Lot No.205852., Abcam 1/200 dilution overnight incubate DETECTION METHOD WesternBreeze chemiluminescent western blot immunodetection kit POSITIVE AND NEGATIVE CONTROLS USED positive control not work ANTIBODY STORAGE CONDITIONS 4 degree for 2 weeks SAMPLE PREPARATION used ProteoExtract, Native membrane protein extraction kit(Calbiochem) AMOUNT OF PROTEIN LOADED 20mg protein/lane ELECTROPHORESIS/GEL CONDITIONS NuPAGE Bis-Tris-Gel, reducing gel, 10%(Invitrogen) TRANSFER AND BLOCKING CONDITIONS WesternBreeze chemiluminescent western blot immunodetection kit SECONDARY ANTIBODY WesternBreeze chemiluminescent western blot immunodetection kit HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 15 DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? No. only antibody new one

    Read More
    Answer

    Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. I have read through your technical questionaire and I have a few comments. OATP2 antibody [MDQ] (ab15442) has been tested for its applications of immunofluorescence, immunohistochemistry (Frozen sections) and immunoprecipitation. To our knowledge we are not aware of how this antibody behaves in western blotting. Therefore unfortunately we cannot guarantee it for this purpose. I am sorry to hear that you have observed differences between the batches of antibody that you have used. This is a monoclonal antibody and I would not expect different antibody preparations to have significant variation in their quality. However, occasional batch to batch variation does exist. Therefore given this I would like to recommend that you perform the optimisation to your protocol; Firstly I would like to suggest that you verify the integrity of your sample and preparation by performing a loading control immunolabelling using an antibody that targets a housekeeping protein such as GAPDH or alpha tubulin. Following on I would like to suggest that you significantly reduce the dilution of the antibody to ~1:20 in view of the antibody dilutions recommended for immunofluorescence and perform an overnight incubation at 4oC using BSA as a blocking agent. This serves to improve the sensitivity of the antibody.

    Read More

    Question

    How exactly were these antibodies tested in IP, IHC, and IF?

    Read More
    Answer

    Thank you for your enquiry. Both antibodies were tested in IP using human liver samples (~20-50 ug) and about 1 ug/ml of antibody used. For IF, human liver and transfected HEK293 whole cell lysates were used. Ab15442 was used at a dilution of 1:20. The fixative was 4% PFA for ~10-15 minutes. Ab15441 was used at 1:100 in IF. If you have any additional questions, please contact us again.

    Read More

    Question

    customer has blasted the peptide seq on the datasheet and thinks it is actualy the sequence for OATP-C and doesnt correspond to OATP8 at all, I checked swissProt and I concur, unless i'm missing something.

    Read More
    Answer

    Thank you for bringing this to our attention. The immunogen sequence is from OATP2, however this product will also recognise OATP8. The data sheet has now been updated to reflect this. Please let me know if you spot any further problems. I apologize for any inconvenience that this may have caused.

    Read More

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