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AB322970

Anti-OAS2 antibody [EPR26734-22] - BSA and Azide free (Detector)

Anti-OAS2 antibody [EPR26734-22] - BSA and Azide free (Detector)

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Rabbit Recombinant Monoclonal OAS2 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

查看别名

2'-5'-oligoadenylate synthase 2, (2-5')oligo(A) synthase 2, 2-5A synthase 2, p69 OAS / p71 OAS, p69OAS / p71OAS, OAS2

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Sandwich ELISA - Anti-OAS2 antibody [EPR26734-22] - BSA and Azide free (Detector) (AB322970)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-OAS2 antibody [EPR26734-22] - BSA and Azide free (Detector) (AB322970)

Sandwich ELISA of ab322968 with the capture antibody ab322969 dilution at 2 ug/mL and detector antibody ab322970 dilution at 0.5 ug/mL.

Sandwich ELISA - Anti-OAS2 antibody [EPR26734-22] - BSA and Azide free (Detector) (AB322970)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-OAS2 antibody [EPR26734-22] - BSA and Azide free (Detector) (AB322970)

Interpolated concentration of native OAS2 was measured at different sample concentrations. Undiluted samples are as follows : stimulated HeLa cell extract (treated with 10 ng/mL IFN-alpha for 16 hours) (62.5 µg/mL) and mock unstimulated HeLa cell extract (62.5 µg/mL). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in antibody diluent CP2 (ab319981). Interpolated concentrations of OAS2 in mock unstimulated HeLa cell extract were below the detectable range of the assay. Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR26734-22

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

sELISA

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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产品详情

ab322970 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human OAS2. The recommended pair for sandwich ELISA is:

Capture: ab322969, Human OAS2 Capture Antibody (unconjugated)

Detector: ab322970, Human OAS2 Detector Antibody (unconjugated)

The reference range value is 0.31-20 ng/mL.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.2 - 7.4 Constituents: PBS
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Interferon-induced, dsRNA-activated antiviral enzyme which plays a critical role in cellular innate antiviral response (PubMed : 10464285, PubMed : 9880569). Activated by detection of double stranded RNA (dsRNA) : polymerizes higher oligomers of 2'-5'-oligoadenylates (2-5A) from ATP which then bind to the inactive monomeric form of ribonuclease L (RNASEL) leading to its dimerization and subsequent activation (PubMed : 10464285, PubMed : 11682059, PubMed : 9880569). Activation of RNASEL leads to degradation of cellular as well as viral RNA, resulting in the inhibition of protein synthesis, thus terminating viral replication (PubMed : 10464285, PubMed : 9880569). Can mediate the antiviral effect via the classical RNASEL-dependent pathway or an alternative antiviral pathway independent of RNASEL (PubMed : 21142819). In addition, it may also play a role in other cellular processes such as apoptosis, cell growth, differentiation and gene regulation (PubMed : 21142819). May act as a negative regulator of lactation, stopping lactation in virally infected mammary gland lobules, thereby preventing transmission of viruses to neonates (By similarity). Non-infected lobules would not be affected, allowing efficient pup feeding during infection (By similarity).
See full target information OAS2
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