重组Anti-OAS1抗体[EPR24824-162] (ab272492)
![Western blot - Anti-OAS1 antibody [EPR24824-162] (ab272492)](https://www.abcam.cn/ps/products/272/ab272492/Images/ab272492-488276-anti-oas1-antibody-epr24824-162-western-blot-human.jpg "Western blot - Anti-OAS1 antibody [EPR24824-162] (ab272492)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR24824-162] to OAS1
- Suitable for: Flow Cyt (Intra), WB, IP
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-OAS1抗体[EPR24824-162]
参阅全部 OAS1 一抗 -
描述
兔单克隆抗体[EPR24824-162] to OAS1 -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WB, IPmore details
不适用于: ICC/IF or IHC-P -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: HeLa treated with 10mg/ml IFN alpha 1 for 16 hours, NCI-H460, Daudi whole cell lysate. Flow Cyt: Daudi cells. IP: NCI-H460 cell. WB-2 : Wild-type HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate, Wild-type HeLa treated with 1000 U/ml IFN beta for 28 hours, whole cell lysate, OAS1 knockout HeLa whole cell lysate and OAS1 knockout HeLa treated with 1000 U/ml IFN beta for 28 hours, whole cell lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR24824-162 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab272492于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
1/50.
|
|
| WB |
1/1000. Predicted molecular weight: 46 kDa.
|
|
| IP |
1/30.
|
| 说明 |
|---|
|
Flow Cyt (Intra)
1/50. |
|
WB
1/1000. Predicted molecular weight: 46 kDa. |
|
IP
1/30. |
靶标
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功能
May play a role in mediating resistance to virus infection, control of cell growth, differentiation, and apoptosis. -
序列相似性
Belongs to the 2-5A synthase family. -
细胞定位
Mitochondrion. Nucleus. Microsome. Endoplasmic reticulum. Associated with different subcellular fractions such as mitochondrial, nuclear, and rough/smooth microsomal fractions. - Information by UniProt
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数据库链接
- Entrez Gene: 4938 Human
- Omim: 164350 Human
- SwissProt: P00973 Human
- Unigene: 524760 Human
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别名
- (2 5')oligo(A) synthetase 1 antibody
- (2-5'')oligo(A) synthase 1 antibody
- 2 5 Oligoadenylate Synthetase 1 antibody
see all
图片
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Western blot - Anti-OAS1 antibody [EPR24824-162] (ab272492)All lanes : Anti-OAS1 antibody [EPR24824-162] (ab272492) at 1/1000 dilution
Lane 1 : Wild-type HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : Wild-type HeLa treated with 1000 U/ml IFN beta for 28 hours, whole cell lysate
Lane 3 : OAS1 knockout HeLa whole cell lysate
Lane 4 : OAS1 knockout HeLa treated with 1000 U/ml IFN beta for 28 hours, whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 46 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?Blocking buffer and concentration : Intercept® (TBS) Blocking Buffer diluted with an equal volume of 0.1% TBS
Diluting buffer and concentration : Intercept® (TBS) Blocking Buffer diluted with an equal volume of 0.1% TBSTLysates/proteins at 20 µg per lane.
Performed under reducing conditions.
False colour image of Western blot: Anti-OAS1antibody [EPR24824-162] (ab272492) staining at 1/1000 dilution, shown in green; Mouse anti-alpha Tubulin antibody [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red.
In Western blot, ab272492 was shown to bind specifically to OAS1. A band was observed at 42 kDa in wild-type HeLa cell lysates with no signal observed at this size in OAS1 knockout cell lysates (ab259009) (knockout cell line ab265578). To generate this image, wild-type and OAS1 knockout HeLa cell lysates were analyzed. First, samples were run on an SDS-PAGE gel then transferred onto an immobilon-FL PVDF membrane. Membranes were blocked in Intercept® (TBS) Blocking Buffer diluted with an equal volume of 0.1% TBS before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/10000 dilution. -
![Western blot - Anti-OAS1 antibody [EPR24824-162] (ab272492)](https://www.abcam.cn/ps/products/272/ab272492/Images/ab272492-476138-anti-oas1-antibody-epr24824162-western-blot-cervix.jpg)
Western blot - Anti-OAS1 antibody [EPR24824-162] (ab272492)All lanes : Anti-OAS1 antibody [EPR24824-162] (ab272492) at 1/1000 dilution
Lane 1 : Untreated HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : HeLa treated with /ml IFN alpha 1 for 16 hours, whole cell lysate 20
Lane 3 : NCI-H460 (human Large Cell Lu Cancer epithelial cell) whole cell lysate 20
Lane 4 : Daudi (human Burkitts lymphoma lymphoblast) whole cell lysate
Lane 5 : LNCaP (human prostate carcinoma epithelial cell) whole cell lysate
Lane 6 : 293T (human embryonic kidney epithelial cell) whole cell lysate
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 46 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBSTThe molecular weight observed is consistent with what has been described in the literature (PMID: 25205466).
Negative control: LNCaP, 293T (PMID: 26997919).
Exposure time: Lane 1-2: 3 minutes Lane 3-6: 37 seconds
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![Immunoprecipitation - Anti-OAS1 antibody [EPR24824-162] (ab272492)](https://www.abcam.cn/ps/products/272/ab272492/Images/ab272492-2-anti-oas1-antibody-epr24824162-immunoprecipitation-ncih460.jpg)
Immunoprecipitation - Anti-OAS1 antibody [EPR24824-162] (ab272492)OAS1 was immunoprecipitated from 0.35 mg NCI-H460 (human Large Cell Lung Cancer epithelial cell) whole cell lysate 10 ug with ab272492 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab272492 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: NCI-H460 (human Large Cell Lung Cancer epithelial cell) whole cell lysate 10 ug
Lane 2: ab272492 IP in NCI-H460 whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab272492 in NCI-H460 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 32 seconds
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![Flow Cytometry (Intracellular) - Anti-OAS1 antibody [EPR24824-162] (ab272492)](https://www.abcam.cn/ps/products/272/ab272492/Images/ab272492-1-anti-oas1-antibody-epr24824162-flow-cytometry-293t.jpg)
Flow Cytometry (Intracellular) - Anti-OAS1 antibody [EPR24824-162] (ab272492)Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized 293T (Human embryonic kidney epithelial cell, left) / Daudi (Human Burkitt's lymphoma lymphoblast, right) cells labelling OAS1 with ab272492 at 1/50 dilution (1ug)/ red (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody. Negative control: 293T(PMID: 19923450).
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (1)
ab272492 被引用在 1 文献中.
- Lee SH et al. The Expression of ephrinA1/ephA2 Receptor Increases in Chronic Rhinosinusitis and ephrinA1/ephA2 Signaling Affects Rhinovirus-Induced Innate Immunity in Human Sinonasal Epithelial Cells. Front Immunol 12:793517 (2021). PubMed: 34975898