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Anti-O-Linked N-Acetylglucosamine抗体[RL2] (ab2739)

  • Datasheet
  • SDS
Reviews (17)Q&A (10)References (136)

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Immunocytochemistry/ Immunofluorescence - Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739)
  • Western blot - Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739)
  • Western blot - Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739)
  • Western blot - Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739)

Key features and details

  • Mouse monoclonal [RL2] to O-Linked N-Acetylglucosamine
  • Suitable for: ICC/IF, WB
  • Reacts with: Rat, Human
  • Isotype: IgG1

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 HRP

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概述

  • 产品名称

    Anti-O-Linked N-Acetylglucosamine抗体[RL2]
    参阅全部 O-Linked N-Acetylglucosamine 一抗
  • 描述

    小鼠单克隆抗体[RL2] to O-Linked N-Acetylglucosamine
  • 宿主

    Mouse
  • 经测试应用

    适用于: ICC/IF, WBmore details
  • 种属反应性

    与反应: Rat, Human
  • 免疫原

    Tissue, cells or virus corresponding to O-Linked N-Acetylglucosamine. Specifically, isolated rat liver nuclear envelopes, which contain 8 O-Linked glycoproteins in the nuclear pore complex

  • 阳性对照

    • ICC-IF: MCF7 cells. WB: Jurkat cells treated with 50 uM PugNAc; SH-SY5Y) whole cell lysate - treated with 50µM z-Pugnac; Rat Liver Nuclear Envelope lysate.
  • 常规说明

    This antibody clone [RL2] is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 6.97% L-Arginine
  • Concentration information loading...
  • 纯度

    Protein G purified
  • 克隆

    单克隆
  • 克隆编号

    RL2
  • 同种型

    IgG1
  • 轻链类型

    kappa
  • 研究领域

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Other
    • Signal Transduction
    • Protein Trafficking
    • Nuclear Import / Export
    • Cell Biology
    • Other Antibodies
    • Other Antibodies

相关产品

  • Alternative Versions

    • Alexa Fluor® 488 Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab201993)
    • Alexa Fluor® 647 Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab201994)
    • HRP Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab201995)
    • Anti-O-Linked N-Acetylglucosamine antibody [RL2] - BSA and Azide free (ab264496)
  • ChIP Related Products

    • ChIP Kit (ab500)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Related Products

    • Tunicamycin, Protein glycosylation inhibitor (ab120296)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab2739于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF (2)
Use a concentration of 5 - 10 µg/ml.
WB (15)
Use a concentration of 1 µg/ml.
说明
ICC/IF
Use a concentration of 5 - 10 µg/ml.
WB
Use a concentration of 1 µg/ml.

靶标

  • 相关性

    Many cellular proteins, including nuclear pore, oncogene, cytoskeletal, heat shock, viral and transcription regulatory proteins contain single O-linked N-acetylglucosamine (O-GlcNAc) residues attached to serine or threonine residues. It has been observed that O-GlcNAc glycosylated proteins tend to be under phosphorylated relative to unglycosylated proteins and that O-GlcNAc bearing proteins tend to be found in multimeric complexes. This has led to the suggestion that O-GlcNAc glycosylation may obscure phosphorylation sites and acts as a signaling mechanism or mediator of signaling.

图片

  • Immunocytochemistry/ Immunofluorescence - Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739)
    Immunocytochemistry/ Immunofluorescence - Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739)

    ab2739 stained in MCF7 cells. Cells were fixed with 4% paraformaldehyde (10min) at room temperature and incubated with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% triton for 1h at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab2739 at 5µg/ml and ab6046 (Rabbit polyclonal to beta Tubulin - Loading Control) at 1/1000 dilution overnight at +4°C. The secondary antibodies were ab150080 (pseudo-colored red) and ab150117 (colored green) used at 1 ug/ml for 1hour at room temperature. DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • Western blot - Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739)
    Western blot - Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739)
    All lanes : Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739) at 1 µg/ml

    Lanes 1 & 3 : Jurkat cells treated with 0 uM PugNAc
    Lane 2 : Jurkat cells treated with 50 uM PugNAc (3 hours)
    Lane 4 : Jurkat cells treated with 4 mM glucosamine and 50 uM PugNAc (3 hours)

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time: 12 minutes


    Jurkat cells were treated with either 50 uM PugNAc (ab144670) or 4 mM glucosamine + 50 uM PugNAc (ab144670) for three hours prior to harvest to stimulate O-linked glycosylation. The expected increase in glycosylation is observed in the treated lanes 2 & 4. 

  • Western blot - Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739)
    Western blot - Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739)This image is courtesy of an anonymous Abreview
    All lanes : Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739) at 1/3000 dilution

    Lane 1 : Human neuroblastoma (SH-SY5Y) whole cell lysate - treated with 50µM z-Pugnac for 24 hours
    Lane 2 : Human neuroblastoma (SH-SY5Y) whole cell lysate - untreated

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP-conjugated horse anti-mouse IgG polyclonal

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time: 30 seconds

    See Abreview

  • Western blot - Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739)
    Western blot - Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739)
    Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739) at 1 µg/ml + Rat Liver Nuclear Envelope at 10 µg

    Secondary
    Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time: 1 minute


    The antibody was tested against the immunogen (isolated rat liver nuclear envelopes, which contain 8 O-linked glycoproteins in the nuclear pore complex).

实验方案

  • ChIP protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (136)

发表研究结果有使用 ab2739?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab2739 被引用在 136 文献中.

  • Erber LN  et al. Iron Deficiency Reprograms Phosphorylation Signaling and Reduces O-GlcNAc Pathways in Neuronal Cells. Nutrients 13:N/A (2021). PubMed: 33430126
  • Verathamjamras C  et al. Aberrant RL2 O-GlcNAc antibody reactivity against serum-IgA1 of patients with colorectal cancer. Glycoconj J 38:55-65 (2021). PubMed: 33608772
  • Shin EM  et al. GREB1: An evolutionarily conserved protein with a glycosyltransferase domain links ERa glycosylation and stability to cancer. Sci Adv 7:N/A (2021). PubMed: 33731348
  • Zhang X  et al. Endogenous glutamate determines ferroptosis sensitivity via ADCY10-dependent YAP suppression in lung adenocarcinoma. Theranostics 11:5650-5674 (2021). PubMed: 33897873
  • Ou W  et al. Hypoxic acclimation improves cardiac redox homeostasis and protects heart against ischemia-reperfusion injury through upregulation of O-GlcNAcylation. Redox Biol 43:101994 (2021). PubMed: 33964586
View all Publications for this product

客户评价及客户问答

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1-10 of 27 Abreviews or Q&A

Western blot abreview for Anti-O-Linked N-Acetylglucosamine antibody [RL2]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Liver)
Gel Running Conditions
Non-reduced Denaturing (10% gel)
Loading amount
30 µg
Specification
Liver
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Apr 19 2021

Western blot abreview for Anti-O-Linked N-Acetylglucosamine antibody [RL2]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Tissue lysate - whole (293T)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
293T
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Mr. Yuanxin Yang

Verified customer

提交于 Feb 02 2021

Western blot abreview for Anti-O-Linked N-Acetylglucosamine antibody [RL2]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (RAW264.7)
Gel Running Conditions
Reduced Denaturing
Loading amount
5 µg
Specification
RAW264.7
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Oct 30 2020

Western blot abreview for Anti-O-Linked N-Acetylglucosamine antibody [RL2]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Liver)
Gel Running Conditions
Non-reduced Denaturing (10% gel)
Loading amount
30 µg
Specification
Liver
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Nov 30 2019

Immunocytochemistry/ Immunofluorescence abreview for Anti-O-Linked N-Acetylglucosamine antibody [RL2]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Liver)
Permeabilization
No
Specification
Liver
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Nov 30 2019

Western blot abreview for Anti-O-Linked N-Acetylglucosamine antibody [RL2]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa)
Gel Running Conditions
Reduced Denaturing (4-20% BioRad gel)
Loading amount
22 µg
Treatment
25 uM OSMI-1, 50 uM OSMI-1, 1 uM Thiamet G, 10 uM Thiamet G, 100 uM Thiamet G
Specification
HeLa
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Oct 31 2019

Western blot abreview for Anti-O-Linked N-Acetylglucosamine antibody [RL2]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (293T)
Gel Running Conditions
Reduced Denaturing (7.5% gel)
Loading amount
100000 cells
Treatment
1. 1.5g/L Glucose 2. 4.5 g/L Glucose for 24hrs
Specification
293T
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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Verified customer

提交于 Aug 26 2019

Immunocytochemistry/ Immunofluorescence abreview for Anti-O-Linked N-Acetylglucosamine antibody [RL2]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (U2OS cells)
Permeabilization
Yes - NP40
Specification
U2OS cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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Verified customer

提交于 Sep 07 2018

Western blot abreview for Anti-O-Linked N-Acetylglucosamine antibody [RL2]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Vascular smooth muscle cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
25 µg
Treatment
100 uM H2O2 for 2 hours
Specification
Vascular smooth muscle cells
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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Verified customer

提交于 Feb 12 2018

Western blot abreview for Anti-O-Linked N-Acetylglucosamine antibody [RL2]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (mast cell type)
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Loading amount
10 µg
Specification
mast cell type
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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提交于 Jun 01 2016

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