AB315460

重组Anti-NUT抗体[EPR28221-46]

Anti-NUT antibody [EPR28221-46]

BOND RX™ Validated
20ul selling size
RabMAb
Recombinant
了解详情

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Anti-NUT antibody [EPR28221-46] is a Rabbit Monoclonal antibody that is used in NUT IHC-P. Suitable for Human samples.

The Nuclear Protein in Testis (NUT/ NUTM1) is specifically expressed in testes. NUT plays an important role in proliferation by regulating expression of TERT, a Telomerase reverse transcriptase implicated in the aging process and tumor formation.

查看别名

C15orf55, NUT, NUTM1, NUT family member 1, Nuclear protein in testis

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUT antibody [EPR28221-46] (AB315460)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUT antibody [EPR28221-46] (AB315460)

Immunohistochemical analysis of paraffin-embedded Panel A HEK-293T cells transfected with a human BRD4-NUT expression vector containing a his tag. Panel B HEK-293T cells transfected with empty vector containing a his tag tissue labeling NUT with ab315460 at 1/5000 (0.103 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Positive staining on (A) HEK-293T transfected with a human BRD4-NUT expression vector, no staining on (B) HEK-293T transfected with empty vector. The section was incubated with ab315460 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUT antibody [EPR28221-46] (AB315460)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling NUT with ab315460 at 1/5000 (0.103 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Negative control : no staining on human liver. The section was incubated with ab315460 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUT antibody [EPR28221-46] (AB315460)

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling NUT with ab315460 at 1/5000 (0.103 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Negative control : no staining on human cerebrum. The section was incubated with ab315460 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUT antibody [EPR28221-46] (AB315460)

Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling NUT with ab315460 at 1/5000 (0.103 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Positive staining on human testis. The section was incubated with ab315460 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUT antibody [EPR28221-46] (AB315460)

Immunohistochemical analysis of paraffin-embedded Tissue Microarray tissue labeling NUT with ab315460, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Table of the human normal TMA tissue depending on the staining. The section was incubated with ab315460 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

不同偶联物与剂型 (1)

Carrier free

Anti-NUT antibody [EPR28221-46] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR28221-46

亚型

IgG

不含载体蛋白

反应种属

Human

应用

IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/5000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Transfected cell line - Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/5000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Conditional Ambient

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The NUT protein known also as Nuclear Protein in Testis is a highly specialized protein with a molecular weight of approximately 112 kDa. Its expression is mostly restricted to post-meiotic germ cells in the testes but aberrant expression occurs in some cancer types. NUT is mainly localized in the nucleus and exhibits involvement in transcriptional regulation due to its structure and function in gene expression processes. It engages in forming fusion proteins notably through translocations which has particular relevance in oncogenesis.

Biological function summary

NUT plays an important role in the regulation of chromatin structure and gene expression. It usually forms a part of the large complex involving histone acetyltransferase which contributes to chromatin remodeling. The protein's interaction with various transcriptional coactivators suggests its function in facilitating access to DNA for transcription machinery. In normal cellular function NUT is limited in expression and activity asserting its importance in specific developmental processes.

Pathways

NUT engages in significant modulation of chromatin remodeling and transcriptional pathways including the BRD4-NUT fusion pathway in cancer biology. This pathway involves the BRD4 protein which forms a fusion with NUT through chromosomal translocations altering normal epigenetic regulation and driving abnormally high transcription of growth-promoting genes. The BRD-NUT fusion therefore creates a powerful oncogenic driver within the transcriptional machinery and chromatin modification pathways.

Aberrations in NUT are closely linked to aggressive forms of cancer such as NUT midline carcinoma. This cancer type arises from the NUT fusion with other proteins like BRD4 or BRD3 resulting in altered gene expression leading to unchecked cell proliferation. The disorders related to NUT reexpression highlight its pathological importance and targeting the BRD-NUT fusion protein has become a focus for therapeutic intervention in treating related malignancies.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Plays a role in the regulation of proliferation. Regulates TERT expression by modulating SP1 binding to TERT promoter binding sites.

See full target information NUTM1

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Abcam Product Promise

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重组Anti-NUT抗体[EPR28221-46]