Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online

Anti-Nurr1抗体[N1404] (ab41917)

Reviews (9)Q&A (7)References (25)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nurr1 antibody [N1404] (ab41917)

    Key features and details

    • Mouse monoclonal [N1404] to Nurr1
    • Suitable for: IHC-P
    • Reacts with: Rat
    • Isotype: IgG2a

    概述

    • 产品名称

      Anti-Nurr1抗体[N1404]
      参阅全部 Nurr1 一抗

    • 描述

      小鼠单克隆抗体[N1404] to Nurr1

    • 宿主

      Mouse

    • 经测试应用

      适用于: IHC-Pmore details

    • 种属反应性

      与反应: Rat

    • 免疫原

      Recombinant fragment: CVQAQYGSSP QGASPASQSY SYHSSGEYSS DFLTPEFVKF SMDLTNTEIT ATTSLPSFST FMDNYSTGYD VKPPCLYQMP LSGQQSSIKV EDIQMHN, corresponding to amino acids 2-99 of Human Nurr1

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • 阳性对照

      • Rat cerebral nerve cell.

    • 常规说明

      This product was changed from ascites to tissue culture supernatant on 3rd April 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab41917于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    IHC-P (6)
    Use at an assay dependent concentration.
    说明
    IHC-P
    Use at an assay dependent concentration.

    靶标

    • 功能

      Transcriptional regulator which is important for the differentiation and maintenance of meso-diencephalic dopaminergic (mdDA) neurons during development. It is crucial for expression of a set of genes such as SLC6A3, SLC18A2, TH and DRD2 which are essential for development of mdDA neurons.

    • 组织特异性

      Expressed in a number of cell lines of T-cell, B-cell and fibroblast origin. Strong expression in brain tissue.

    • 序列相似性

      Belongs to the nuclear hormone receptor family. NR4 subfamily.
      Contains 1 nuclear receptor DNA-binding domain.

    • 发展阶段

      Rapidly and only very transiently expressed after cell activation, during the G0-G1 transition of the cell cycle.

    • 细胞定位

      Nucleus.
    • Information by UniProt

    • 数据库链接

      • 别名

        • HZF 3 antibody
        • HZF3 antibody
        • Immediate-early response protein NOT antibody
        • Intermediate early receptor protein antibody
        • NGFI B/nur77 beta type transcription factor homolog antibody
        • NOT antibody
        • Nr4a2 antibody
        • NR4A2_HUMAN antibody
        • nuclear receptor of T cells antibody
        • nuclear receptor related 1 antibody
        • Nuclear receptor subfamily 4 group A member 2 antibody
        • Nur related protein 1 homolog antibody
        • nur related protein-1, human homolog of antibody
        • Nurr 1 antibody
        • Orphan nuclear receptor NR4A2 antibody
        • Orphan nuclear receptor NURR1 antibody
        • RNR 1 antibody
        • RNR1 antibody
        • T cell nuclear receptor NOT antibody
        • T-cell nuclear receptor NOT antibody
        • TINUR antibody
        • Transcriptionally inducible nuclear receptor antibody
        • Transcriptionally inducible nuclear receptor related antibody
        • Transcriptionally inducible nuclear receptor related 1 antibody
        • Transcriptionally-inducible nuclear receptor antibody
        see all

      图片

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nurr1 antibody [N1404] (ab41917)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nurr1 antibody [N1404] (ab41917)

        ab41917 staining Nurr1 rat cerebral nerve cell paraffin section (10 ug/mL) by immunohistochemistry, formalin-fixed paraffin embedded sections.

        This image was generated using the ascites version of the product.

      文献 (25)

      发表研究结果有使用 ab41917？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab41917 被引用在 25 文献中.

      • Yu S  et al. BMS-470539 Attenuates Oxidative Stress and Neuronal Apoptosis via MC1R/cAMP/PKA/Nurr1 Signaling Pathway in a Neonatal Hypoxic-Ischemic Rat Model. Oxid Med Cell Longev 2022:4054938 (2022). PubMed: 35140838
      • Xu 许潇依 X  et al. CREB inactivation by HDAC1/PP1γ contributes to dopaminergic neurodegeneration in Parkinson's disease. J Neurosci 42:4594-604 (2022). PubMed: 35501151
      • Zarei M  et al. Nuclear Receptor 4A2 (NR4A2/NURR1) Regulates Autophagy and Chemoresistance in Pancreatic Ductal Adenocarcinoma. Cancer Res Commun 1:65-78 (2021). PubMed: 35582016
      • Beiki R  et al. Synergistic Effects of Combined Nurr1 Overexpression and Natural Inducers on the More Efficient Production of Dopaminergic Neuron-Like Cells From Stem Cells. Front Cell Neurosci 15:803272 (2021). PubMed: 35087379
      • Rey F  et al. Mitochondrial Metabolism as Target of the Neuroprotective Role of Erythropoietin in Parkinson's Disease. Antioxidants (Basel) 10:N/A (2021). PubMed: 33467745
      View all Publications for this product

      客户评价及客户问答

      Show All 评价 Q&A
      提交评价 提交问题

      1-10 of 16 Abreviews or Q&A

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Nurr1 antibody [N1404]

       Good
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Human Tissue sections (colon - submucosal vessels)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: citrate buffer
      Specification
      colon - submucosal vessels
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
      Fixative
      Formaldehyde
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 Jun 30 2023

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Nurr1 antibody [N1404]

       Average
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Mouse Tissue sections (embryonic mouse midbrain)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: 1x DAKO antigen retrieval buffer
      Permeabilization
      Yes - 0.3% Triton X-100 in PBS for 5 minutes
      Specification
      embryonic mouse midbrain
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 21°C
      Fixative
      Paraformaldehyde
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      DR. Laura Lahti

      Verified customer

      提交于 Dec 04 2021

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Nurr1 antibody [N1404]

       Excellent
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Mouse Tissue sections (Brain)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
      Permeabilization
      No
      Specification
      Brain
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
      Fixative
      Paraformaldehyde
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 Aug 12 2020

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Nurr1 antibody [N1404]

       Excellent
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Human Tissue sections (Brain)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
      Permeabilization
      No
      Specification
      Brain
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
      Fixative
      Paraformaldehyde
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 Aug 12 2020

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Nurr1 antibody [N1404] - ChIP Grade

       Inconclusive
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Mouse Tissue sections (Brain)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Citric acid
      Permeabilization
      No
      Specification
      Brain
      Blocking step
      BSA as blocking agent for 10 minute(s) · Concentration: 1%
      Fixative
      Formaldehyde
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      MR. Carl Hobbs

      Verified customer

      提交于 Jan 04 2016

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Nurr1 antibody [N1404] - ChIP Grade

       Inconclusive
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Rat Tissue sections (Brain)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Citric acid
      Permeabilization
      No
      Specification
      Brain
      Blocking step
      BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
      Fixative
      Formaldehyde
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      MR. Carl Hobbs

      Verified customer

      提交于 Jan 04 2016

      ChIP abreview for Anti-Nurr1 antibody [N1404]

       Good
      Abreviews
      abreview image
      Application
      ChIP
      Detection step
      Real-time PCR
      Sample
      Mouse Cell lysate - whole cell (mouse microglia cells)
      Specification
      mouse microglia cells
      Type
      Cross-linking (X-ChIP)
      Duration of cross-linking step: 10 minute(s) and 0 second(s)
      Specification of the cross-linking agent: 1% formaldehyde
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 Sep 09 2013

      Western blot abreview for Anti-Nurr1 antibody [N1404]

       Good
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (macrophage)
      Loading amount
      200 µg
      Specification
      macrophage
      Treatment
      20uM compound X and IP perfomred
      Gel Running Conditions
      Reduced Denaturing (10%)
      Blocking step
      Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 26°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 Sep 13 2012

      Immunoprecipitation abreview for Anti-Nurr1 antibody [N1404]

       Excellent
      Abreviews
      abreview image
      Application
      Immunoprecipitation
      Sample
      Mouse Cell lysate - whole cell (RAW cell (mouse macrophage))
      Total protein in input
      200 µg
      Specification
      RAW cell (mouse macrophage)
      Immuno-precipitation step
      Protein A/G
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 Sep 07 2012

      Question

      Product code: 41917
      Lot number: GR16596-11
      Inquiry: Hello, We ordered Nurr1 antibody (Anti-Nurr1 antibody [N1404] (ab41917), Lot GR16596-11 and we have tried it in different conditions with no positive result. These were the conditions: IHC-paraffin PFA fixed samples, ethanol grade dehydration, rotihistol, paraffin and embedding. 10 μM sections Deparaffination in rotihistol followed by ethanol grades All washes in PBS Antigen retrieval with citric acid and incubation in microwave. Endogenous peroxidases inactivation by incubating samples in 3% H2O2 for 30 min. Blocking: 10% normal goat serum, 0,1 % triton, 1% BSA in PBS or 0,1 % triton, 1% BSA in PBS, or 1 % triton, 1% BSA in PBS 1-2 hours Primary antibody: 10% normal goat serum, 0,1 % triton, 1% BSA in PBS or 0,1 % triton, 1% BSA in PBS overnight 4°C 1/100 or 1/400 Secondary antibody: Biotinilated goat anti-mouse secondary antibody for 2 hours room temperature 1/600 (standardized for other stainings) Amplification: VECTASTAIN® ABC Kit Detection with DAB Peroxidase Substrate Kit, 3,3’-diaminobenzidine (Vector laboratoires) Immunofluorescence in cryosections: 4% PFA fixed samples, 30% sucrose in PBS overnight, then embedding in OCT. 10 μM sections All washes in PBS Blocking: 10% normal goat serum, 0,1 % triton, 1% BSA in PBS or 0,1 % triton, 1% BSA in PBS 1-2 hours Primary antibody: 10% normal goat serum, 0,1 % triton, 1% BSA in PBS or 0,1 % triton, 1% BSA in PBS overnight 4°C 1/100 or 1/400 Secondary antibody: Goat anti-mouse secondary antibody labelled with Cy3 or Cy2 for 2 hours room temperature 1:200 In both situations we obtained non specific staining, specially in blood vessels. Specifical signal was not found. We used midbrain tissue for which signal should be present in dopaminergic neurons. Stainings were performed at the same with other antibodies for which we obtained specific signal.

      Read More
      Answer

      Thank you for your email. I am sorry to hear that you have been experiencing problems with this antibody.
      I have read the details you have kindly provided and have following further questions for better understanding of the problem;
      - For how long the sections were heated in microwave?
      - Could you provide the image?
      - Have you done any sort of troubleshooting?
      - Which other antibody has been used? Is it against Nurr1 or other proteins?
      - What is the purchase order number?
      The protein Nurr1 has been shown to be observed in T-cells as well as B cells. http://www.uniprot.org/uniprot/P43354. Could you please explain why it should not stain blood vessels.
      Thank you very much for your cooperation. I will look forward to hearing from you soon.

      Read More

      1-10 of 16 Abreviews or Q&A

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com