重组Anti-NUP98抗体[EPR6678] -核Pore Marker (ab124980)
![Western blot - Anti-NUP98 antibody [EPR6678] - Nuclear Pore Marker (ab124980)](https://www.abcam.cn/ps/products/124/ab124980/Images/ab124980-139075-anti-nup98-antibody-epr6678-nuclear-pore-marker-western-blot.jpg "Western blot - Anti-NUP98 antibody [EPR6678] - Nuclear Pore Marker (ab124980)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6678] to NUP98 - Nuclear Pore Marker
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-NUP98抗体[EPR6678] -核Pore Marker
参阅全部 NUP98 一抗 -
描述
兔单克隆抗体[EPR6678] to NUP98 -核Pore Marker -
宿主
Rabbit -
特异性
ab124980 recognises Nuclear pore complex protein Nup96 of Nuclear pore complex protein Nup98-Nup96. -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details
不适用于: Flow Cyt or IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human NUP98 aa 1600-1700. The exact sequence is proprietary. The immunogen sequence is within Nuclear pore complex protein Nup96 of the Nuclear pore complex protein Nup98-Nup96.
Database link: P52948 -
阳性对照
- HeLa, SH-SY5Y, and Caco-2 cell lysates and Human colon tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
纯化说明
Not purified; provided in cell supernatant and storage buffer. -
克隆
单克隆 -
克隆编号
EPR6678 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab124980于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 188 kDa.
|
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| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Antigen retrieval is recommended. |
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| ICC/IF |
1/50 - 1/100.
|
| 说明 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 188 kDa. |
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval is recommended. |
|
ICC/IF
1/50 - 1/100. |
靶标
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功能
Nup98 and Nup96 play a role in the bidirectional transport across the nucleoporin complex (NPC). The repeat domain in Nup98 has a direct role in the transport. -
疾病相关
Note=A chromosomal aberration involving NUP98 is found in a form of acute myeloid leukemia. Translocation t(7;11)(p15;p15) with HOXA9. Translocation t(11;17)(p15;p13) with PHF23.
Note=A chromosomal aberration involving NUP98 is found in childhood acute myeloid leukemia. Translocation t(5;11)(q35;p15.5) with NSD1. Translocation t(8;11)(p11.2;p15) with WHSC1L1.
Note=A chromosomal aberration involving NUP98 is found in a form of therapy-related myelodysplastic syndrome. Translocation t(11;20)(p15;q11) with TOP1.
Note=A chromosomal aberration involving NUP98 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(3;11)(q12.2;p15.4) with LNP1.
Note=A chromosomal aberration involving NUP98 is associated with pediatric acute myeloid leukemia (AML) with intermediate characteristics between M2-M3 French-American-British (FAB) subtypes. Translocation t(9;11)(p22;p15) with PSIP1/LEDGF. The chimeric transcript is an in-frame fusion of NUP98 exon 8 to PSIP1/LEDGF exon 4. -
序列相似性
Belongs to the nucleoporin GLFG family.
Contains 1 peptidase S59 domain. -
结构域
Contains G-L-F-G repeats. -
翻译后修饰
Isoform 1 to isoform 4 are autoproteolytically cleaved to yield Nup98 and Nup96 or Nup98 only, respectively. Cleaved Nup98 is necessary for the targeting of Nup98 to the nuclear pore and the interaction with Nup96. -
细胞定位
Nucleus > nuclear pore complex. Nucleus membrane. Nup96 is localized to the nucleoplasmic side of the nuclear pore complex, at or near the nucleoplasmic basket. - Information by UniProt
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数据库链接
- Entrez Gene: 4928 Human
- Omim: 601021 Human
- SwissProt: P52948 Human
- Unigene: 524750 Human
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别名
- 96 kDa nucleoporin antibody
- 98 kDa nucleoporin antibody
- ADAR2 antibody
see all
图片
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Western blot - Anti-NUP98 antibody [EPR6678] - Nuclear Pore Marker (ab124980)All lanes : Anti-NUP98 antibody [EPR6678] - Nuclear Pore Marker (ab124980) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : SH-SY5Y cell lysate
Lane 3 : Caco-2 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 188 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [EPR6678] - Nuclear Pore Marker (ab124980)](https://www.abcam.cn/ps/products/124/ab124980/Images/ab124980-354633-AntiNUP98-antibody-EPR6678--Nuclear-Pore-Marker-immunocytochemistry.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [EPR6678] - Nuclear Pore Marker (ab124980)ab124980 staining NUP98 in SH-SY5Y, human neuroblastoma epithelial cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde, permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody (1/500). Ab150077, an Alexa Fluor® 594-conjugated Goat anti rabbit (1:1000) (ab150077) was used as the secondary antibody. Counterstained with ab195889, an Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (1/100). Nuclear counterstained with DAPI.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUP98 antibody [EPR6678] - Nuclear Pore Marker (ab124980)](https://www.abcam.cn/ps/products/124/ab124980/Images/ab124980-139074-anti-nup98-antibody-epr6678-nuclear-pore-marker-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUP98 antibody [EPR6678] - Nuclear Pore Marker (ab124980)ab124980, at 1/50 to 1/100, staining NUP98 in Human colon tissue by immunohistochemistry [Paraffin Embedded Tissues (IHC-P)].
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-NUP98 antibody [EPR6678] - Nuclear Pore Marker (ab124980)](https://www.abcam.cn/ps/products/124/ab124980/Images/ab124980-4-benefits-of-recombinant-antibodies.png)
Anti-NUP98 antibody [EPR6678] - Nuclear Pore Marker (ab124980)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab124980 被引用在 1 文献中.
- Light WH et al. A conserved role for human Nup98 in altering chromatin structure and promoting epigenetic transcriptional memory. PLoS Biol 11:e1001524 (2013). PubMed: 23555195