AB179894

Anti-NUP98抗体[13C2]

Name: NUP98抗体[13C2] (ab179894)| Abcam中文官网
Brand: Abcam Limited
SKU: AB179894
Price: 9609 CNY
Availability: InStock

Anti-NUP98 antibody [13C2]

Be the first to review this product! Submit a review

(1 Publication)

Mouse Monoclonal NUP98 antibody. Suitable for WB, ICC/IF and reacts with Schizosaccharomyces pombe, Saccharomyces cerevisiae, Tetrahymena, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human NUP98 aa 600-700.

查看别名

ADAR2, NUP98, Nuclear pore complex protein Nup98-Nup96

8 Images

Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [13C2] (AB179894)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [13C2] (AB179894)

Immunofluorescent analysis of HeLa cells labeling NUP98 with ab179894 at 0.5 μg/ml in PBS (top panel). DAPI staining (middle panel). 4 μg/ml of Alexa488-labeled anti-mouse IgG was used as secondary antibody. The color image represents merged images of ab179894 (green) with DAPI (magenta).

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [13C2] (AB179894)

Immunofluorescent analysis of S. pombe cells labeling NUP98 with ab179894 (top panel). DAPI (middle panel). The color image represents merged images of ab179894 (green) with DAPI (magenta). Dotted lines represent the outlines of cells.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [13C2] (AB179894)

Immunofluorescent analysis of Tetrahymena thermophila cells labeling NUP98 with ab179894 at 0.5 μg/ml (top panel). DAPI (middle panel). The color image represents merged images of ab179894 (green) with DAPI (magenta).4 μg/ml of Alexa488-labelled anti-mouse IgG was used as secondary antibody. Dotted lines represent the outlines of cells. The insert is a magnified image showing the position of the micronucleus.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [13C2] (AB179894)

Immunofluorescent analysis of S. cerevisiae cells labeling NUP98 with ab179894 at 1/100 dilution (top panel). DAPI (middle panel). The color image represents a merged image of ab179894 (green) with DAPI (magenta). Dotted lines represent the outlines of cells.

Supplier Data

Western blot - Anti-NUP98 antibody [13C2] (AB179894)

ab179894 crossreacts with multiple nucleoporins of S. cerevisiae.

Asterisks represent uncharacterized proteins.

All lanes:

Supernatant of hybridoma culture medium of ab179894 at 1/10 dilution

All lanes:

S. cerevisiae cell extract

Predicted band size: 198 kDa

false

Supplier Data

Western blot - Anti-NUP98 antibody [13C2] (AB179894)

All lanes:

Western blot - Anti-NUP98 antibody [13C2] (ab179894) at 0.4 µg/mL

All lanes:

HeLa cell extract

Secondary

All lanes:

HRP-labeled anti-mouse IgG at 0.4 µg/mL

Predicted band size: 198 kDa

true

Supplier Data

Western blot - Anti-NUP98 antibody [13C2] (AB179894)

Open arrowheads represent the positions of MacNup98A. The 125-kDa band corresponds to GFP-MacNup98A. Diamonds and asterisks represent uncharacterized proteins.

All lanes:

Western blot - Anti-NUP98 antibody [13C2] (ab179894)

Lane 1:

Tetrahymena thermophila wild type cell extract

Lane 2:

Tetrahymena thermophila cell extract ectopically-expressing GFP-MacNup98A (in addition to endogenous MacNup98A)

Predicted band size: 198 kDa

false

Supplier Data

Western blot - Anti-NUP98 antibody [13C2] (AB179894)

All lanes:

Western blot - Anti-NUP98 antibody [13C2] (ab179894)

Lane 1:

S. pombe cell extract

Lane 2:

NUP98-GFP-expressing S. pombe cell extract

Predicted band size: 198 kDa

false

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

13C2

亚型

IgG1

轻链类型

kappa

不含载体蛋白

反应种属

Human, Saccharomyces cerevisiae, Tetrahymena, Schizosaccharomyces pombe

应用

ICC/IF, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

Synthetic Peptide within Human NUP98 aa 1-50. Database link P52948

style

left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.5 µg/mL", "ICCIF-species-notes": "" }, "Saccharomyces cerevisiae": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.5 µg/mL", "ICCIF-species-notes": "" }, "Schizosaccharomyces pombe": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.5 µg/mL", "ICCIF-species-notes": "" }, "Tetrahymena": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.5 µg/mL", "ICCIF-species-notes": "" } } }

style

left-column

style

left-column

性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein G

纯化说明

ab179894 is filter-sterilized.

存储溶液

pH: 6 - 8.5 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

style

left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The NUP98 protein also known as nucleoporin 98 plays an important role in cellular mechanics. It forms a part of the nuclear pore complex important for regulating nucleocytoplasmic transport. With an approximate mass of 98 kDa NUP98 expresses mainly in the nucleus across various cell types. Alternative splicing of the NUP98 gene can lead to different isoforms. In some research contexts NUP98 is also referred to by related terms such as NUP96. Its expression appears widespread in many tissues replacing or complementing other nucleoporins.

Biological function summary

The NUP98 protein contributes to nuclear-cytoplasmic transport processes as part of the nuclear pore complex. In association with other nucleoporins NUP98 acts in cargo transport between the nucleus and cytoplasm facilitating the movement of RNA and proteins. It also participates in the assembly and disassembly cycles of the nuclear pore complex (NPC). Known interactions include those with components like the 13C2 complex and other mRNA export factors. NUP98's function impacts NPC architecture and its transport efficiency.

Pathways

NUP98 has essential roles beyond mechanical transport. It integrates into the mRNA export pathway collaborating with proteins like CRM1 (exportin-1) to regulate mRNA export. Additionally it plays a role in gene expression regulation by binding to gene promoters involving pathways linked to protein synthesis and cell cycle regulation. Connections with histone modifications suggest its involvement in chromatin remodeling linking it further to the regulation of gene expression pathways.

NUP98 associations are significant. It is implicated in certain hematological malignancies like acute myeloid leukemia (AML) where it forms fusion proteins with partners such as HOXA9 and NSD1. Genetic rearrangements involving NUP98 contribute to oncogenesis altering normal cell function. Other conditions including dysregulation in autoimmune diseases suggest potential links to aberrant signaling pathways. Understanding these disease links involves studying interactions with proteins like MLL another significant player in leukemia pathogenesis.

style

left-column

style

left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

靶点信息

Plays a role in the nuclear pore complex (NPC) assembly and/or maintenance. NUP98 and NUP96 are involved in the bidirectional transport across the NPC (PubMed : 33097660). May anchor NUP153 and TPR to the NPC. In cooperation with DHX9, plays a role in transcription and alternative splicing activation of a subset of genes (PubMed : 28221134). Involved in the localization of DHX9 in discrete intranuclear foci (GLFG-body) (PubMed : 28221134).. (Microbial infection) Interacts with HIV-1 capsid protein P24 and nucleocapsid protein P7 and may thereby promote the integration of the virus in the host nucleus (in vitro) (PubMed : 23523133). Binding affinity to HIV-1 CA-NC complexes bearing the capsid change Asn-74-Asp is reduced (in vitro) (PubMed : 23523133).

See full target information NUP98

style

left-column

文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Monoclonal antibodies in immunodiagnosis and immunotherapy 32:81-90 PubMed23607342

2013

Monoclonal antibodies recognize gly-leu-phe-gly repeat of nucleoporin nup98 of tetrahymena, yeasts, and humans.

Applications

Unspecified application

Species

Unspecified reactive species

Masaaki Iwamoto,Haruhiko Asakawa,Chizuru Ohtsuki,Hiroko Osakada,Takako Koujin,Yasushi Hiraoka,Tokuko Haraguchi

View all publications

style

left-column

style

left-column

style

right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NUP98抗体[13C2]

Anti-NUP98 antibody [13C2]

Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [13C2] (AB179894)

Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [13C2] (AB179894)

Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [13C2] (AB179894)

Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [13C2] (AB179894)

Western blot - Anti-NUP98 antibody [13C2] (AB179894)

Western blot - Anti-NUP98 antibody [13C2] (AB179894)

Western blot - Anti-NUP98 antibody [13C2] (AB179894)

Western blot - Anti-NUP98 antibody [13C2] (AB179894)

关键信息

宿主种属

克隆

克隆号

亚型

轻链类型

不含载体蛋白

反应种属

应用

免疫原