JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB137092

重组Anti-NUP50抗体[EPR9526]

Anti-NUP50 antibody [EPR9526]

4

(1 Review)

|

(6 Publications)

Rabbit Recombinant Monoclonal NUP50 antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 6 publications.

查看别名

NPAP60L, PRO1146, NUP50, Nuclear pore complex protein Nup50, 50 kDa nucleoporin, Nuclear pore-associated protein 60 kDa-like, Nucleoporin Nup50

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-NUP50 antibody [EPR9526] (AB137092)
  • ICC/IF

AbReview32462****

Immunocytochemistry/ Immunofluorescence - Anti-NUP50 antibody [EPR9526] (AB137092)

ab137092 (1/200) staining NUP50 in HeLa Cells (green). Cells were fixed in methanol and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.

This image is courtesy of an abreview submitted by Kirk McManus, Univ. of Manitoba/Cancer Care MICB, Canada

Immunocytochemistry/ Immunofluorescence - Anti-NUP50 antibody [EPR9526] (AB137092)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-NUP50 antibody [EPR9526] (AB137092)

Immunofluorescent analysis of HeLa cells labelling NUP50 with ab137092 at 1/250 dilution.

Western blot - Anti-NUP50 antibody [EPR9526] (AB137092)
  • WB

Unknown

Western blot - Anti-NUP50 antibody [EPR9526] (AB137092)

All lanes:

Western blot - Anti-NUP50 antibody [EPR9526] (ab137092) at 1/1000 dilution

Lane 1:

Jurkat cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

293T cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 50 kDa

false

不同偶联物与剂型 (2)

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR9526

亚型

IgG

不含载体蛋白

No

反应种属

Human

应用

ICC/IF, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column

产品详情

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
style
left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Nucleoporin 50 also known as NUP50 is a nucleoporin protein that plays an essential role in the nuclear pore complex. With a molecular mass of approximately 50 kDa it is primarily found in the nuclear envelope. NUP50 functions mechanically as part of the assembly and regulation of nuclear transport facilitating the translocation of macromolecules between the nucleus and cytoplasm.
Biological function summary

This protein serves an integral role in nucleocytoplasmic transport functioning as a component of the nuclear pore complex. NUP50 interacts with karyopherins which are transport receptors to regulate the directionality and specificity of molecular trafficking between the nucleus and cytoplasm. It also helps disassemble importin-cargo complexes within the nucleus important for the recycling of importins.

Pathways

NUP50 participates in the nuclear transport pathways that are vital for gene expression and cell cycle regulation. It associates closely with the other nucleoporins and transport protein receptors such as Importin-β within these pathways. NUP50 also contributes to DNA replication and repair pathways by facilitating the import of necessary proteins into the nucleus.

NUP50 is associated with certain cancers and neurodegenerative disorders. Its altered expression can impact the normal functioning of the nuclear transport system leading to these pathological conditions. Dysregulation or mutations of NUP50 can interact with other nucleoporins like NUP98 connecting it to disorders involving abnormal cellular proliferation and differentiation.
style
left-column
style
left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

靶点信息

Component of the nuclear pore complex that has a direct role in nuclear protein import (PubMed : 20016008). Actively displaces NLSs from importin-alpha, and facilitates disassembly of the importin-alpha : beta-cargo complex and importin recycling (PubMed : 20016008). Interacts with regulatory proteins of cell cycle progression including CDKN1B (By similarity). This interaction is required for correct intracellular transport and degradation of CDKN1B (By similarity).
See full target information NUP50
style
left-column

文献 (6)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:342 PubMed36670122

2023

Integrative genetic analysis illuminates ALS heritability and identifies risk genes.

Applications

Unspecified application

Species

Unspecified reactive species

Salim Megat,Natalia Mora,Jason Sanogo,Olga Roman,Alberto Catanese,Najwa Ouali Alami,Axel Freischmidt,Xhuljana Mingaj,Hortense De Calbiac,François Muratet,Sylvie Dirrig-Grosch,Stéphane Dieterle,Nick Van Bakel,Kathrin Müller,Kirsten Sieverding,Jochen Weishaupt,Peter Munch Andersen,Markus Weber,Christoph Neuwirth,Markus Margelisch,Andreas Sommacal,Kristel R Van Eijk,Jan H Veldink,Géraldine Lautrette,Philippe Couratier,Agnès Camuzat,Isabelle Le Ber,Maurizio Grassano,Adriano Chio,Tobias Boeckers,Albert C Ludolph,Francesco Roselli,Deniz Yilmazer-Hanke,Stéphanie Millecamps,Edor Kabashi,Erik Storkebaum,Chantal Sellier,Luc Dupuis

Cell reports 40:111106 PubMed35858577

2022

Nuclear RNA binding regulates TDP-43 nuclear localization and passive nuclear export.

Applications

Unspecified application

Species

Unspecified reactive species

Lauren Duan,Benjamin L Zaepfel,Vasilisa Aksenova,Mary Dasso,Jeffrey D Rothstein,Petr Kalab,Lindsey R Hayes

The EMBO journal 40:e108788 PubMed34725842

2021

The nucleoporin Nup50 activates the Ran guanine nucleotide exchange factor RCC1 to promote NPC assembly at the end of mitosis.

Applications

Unspecified application

Species

Unspecified reactive species

Guillaume Holzer,Paola De Magistris,Cathrin Gramminger,Ruchika Sachdev,Adriana Magalska,Allana Schooley,Anja Scheufen,Birgitt Lennartz,Marianna Tatarek-Nossol,Hongqi Lue,Monika I Linder,Ulrike Kutay,Christian Preisinger,Daniel Moreno-Andres,Wolfram Antonin

Neuron 107:1124-1140.e11 PubMed32673563

2020

GC Repeat RNA Initiates a POM121-Mediated Reduction in Specific Nucleoporins in C9orf72 ALS/FTD.

Applications

Unspecified application

Species

Unspecified reactive species

Alyssa N Coyne,Benjamin L Zaepfel,Lindsey Hayes,Boris Fitchman,Yuval Salzberg,En-Ching Luo,Kelly Bowen,Hannah Trost,Stefan Aigner,Frank Rigo,Gene W Yeo,Amnon Harel,Clive N Svendsen,Dhruv Sareen,Jeffrey D Rothstein

Cell 173:958-971.e17 PubMed29628143

2018

Stress Granule Assembly Disrupts Nucleocytoplasmic Transport.

Applications

Unspecified application

Species

Unspecified reactive species

Ke Zhang,J Gavin Daigle,Kathleen M Cunningham,Alyssa N Coyne,Kai Ruan,Jonathan C Grima,Kelly E Bowen,Harsh Wadhwa,Peiguo Yang,Frank Rigo,J Paul Taylor,Aaron D Gitler,Jeffrey D Rothstein,Thomas E Lloyd

Developmental cell 43:141-156.e7 PubMed29065306

2017

Mitotic Disassembly of Nuclear Pore Complexes Involves CDK1- and PLK1-Mediated Phosphorylation of Key Interconnecting Nucleoporins.

Applications

Unspecified application

Species

Unspecified reactive species

Monika I Linder,Mario Köhler,Paul Boersema,Marion Weberruss,Cornelia Wandke,Joseph Marino,Caroline Ashiono,Paola Picotti,Wolfram Antonin,Ulrike Kutay
View all publications
style
left-column
style
left-column
style
right-column

替代产品

Primary Antibodies

AB85915

Anti-NUP50 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUP50 antibody (AB85915)

  • 0
  • 0
Primary Antibodies

AB151567

Anti-NUP50 antibody [EPR9525]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-NUP50 antibody [EPR9525] (AB151567)

  • 0
  • 0
Primary Antibodies

AB240121

Anti-NUP50 antibody [EPR9525] - BSA and Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUP50 antibody [EPR9525] - BSA and Azide free (AB240121)

  • 0
  • 0

搭配使用产品

Primary Antibodies

AB24700

Anti-Nup153 antibody [QE5]

Immunocytochemistry/ Immunofluorescence - Anti-Nup153 antibody [QE5] (AB24700)

  • 4
  • 5
Primary Antibodies

AB24609

Anti-Nuclear Pore Complex Proteins antibody [Mab414]

Immunocytochemistry/ Immunofluorescence - Anti-Nuclear Pore Complex Proteins antibody [Mab414] (AB24609)

  • 4
  • 12
Primary Antibodies

AB70497

Anti-NUP214 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUP214 antibody (AB70497)

  • 4
  • 4
Primary Antibodies

AB8227

Anti-beta Actin antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Western blot - Anti-beta Actin antibody - Loading Control (AB8227)

  • 5
  • 104

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com