AB157090

Anti-NUP205抗体

Name: NUP205抗体 (ab157090)| Abcam中文官网
Brand: Abcam Limited
SKU: AB157090
Price: 4606 CNY
Availability: InStock

Anti-NUP205 antibody

Be the first to review this product! Submit a review

(4 Publications)

Rabbit Polyclonal NUP205 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human NUP205 aa 1750-1800.

查看别名

C7orf14, KIAA0225, NUP205, Nuclear pore complex protein Nup205, 205 kDa nucleoporin, Nucleoporin Nup205

2 Images

Unknown

Immunoprecipitation - Anti-NUP205 antibody (AB157090)

ab157090 at 1 µg/ml detecting NUP205 in 293T whole cell lysate by WB following IP.

Lane 1 : IP with an antibody which recognizes NUP205
Lane 2 : ab157090 at 6 µg/mg of lysate
Lane 3 : Control IgG.

In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.

Detection : Chemiluminescence with an exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-NUP205 antibody (ab157090)

Predicted band size: 228 kDa

false

Unknown

Western blot - Anti-NUP205 antibody (AB157090)

All lanes:

Western blot - Anti-NUP205 antibody (ab157090) at 0.1 µg/mL

Lane 1:

293T whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 50 µg

Lane 4:

Jurkat whole cell lysate at 50 µg

Predicted band size: 228 kDa

true

Exposure time: 3min

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Human

应用

WB, IP

applications

免疫原

Synthetic Peptide within Human NUP205 aa 1750-1800. The exact immunogen used to generate this antibody is proprietary information.

Q92621

style

left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Primates": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

style

left-column

性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

纯化说明

ab157090 was affinity purified using an epitope specific to NUP205 immobilized on solid support.

存储溶液

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

运输条件

Blue Ice

储存信息

Avoid freeze / thaw cycle|Do Not Freeze

style

left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The protein NUP205 also known as nucleoporin 205 kDa is an important structural component of the nuclear pore complex (NPC). It has a molecular mass of approximately 205 kDa. NUP205 is expressed ubiquitously in eukaryotic cells as part of the nuclear envelope where it is an essential link in regulating the transport of molecules between the nucleus and the cytoplasm. The expression patterns show its presence across different cell types indicating its fundamental role in cellular function.

Biological function summary

NUP205 is an essential component of the nuclear pore complex contributing to nuclear-cytoplasmic transport. The protein works in concert with other nucleoporins to form the scaffold of the NPC facilitating the bidirectional movement of proteins and RNA. NUP205 as part of this large multiprotein complex plays a significant role in maintaining cellular compartmentalization and influencing gene expression patterns by controlling the transit of regulatory molecules in and out of the nucleus.

Pathways

NUP205 interacts significantly within the nuclear transport pathway. It engages intimately in the regulation of protein and RNA shuttling which influences gene expression and cell cycle processes. NUP205 associates with other nucleoporins such as NUP214 and NUP62 participating in processes like signal transduction and RNA processing. Its functionality supports broader cellular pathways by acting as a gatekeeper ensuring essential factors access or exit the nucleus as needed.

NUP205 has connections to various pathological conditions stemming from disrupted nucleocytoplasmic transport. It is implicated in acute myeloid leukemia (AML) where aberrant expression or mutations of NUP205 can disrupt normal cellular transport functions possibly in association with NUP214. Additionally defects in NUP205 function have been linked to certain neurodegenerative conditions highlighting its role in maintaining neuronal cell homeostasis. Understanding NUP205's molecular behavior offers potential insights into the pathogenesis of these diseases and targets for therapeutic intervention.

style

left-column

style

left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

靶点信息

Plays a role in the nuclear pore complex (NPC) assembly and/or maintenance (PubMed : 9348540). May anchor NUP62 and other nucleoporins, but not NUP153 and TPR, to the NPC (PubMed : 15229283). In association with TMEM209, may be involved in nuclear transport of various nuclear proteins in addition to MYC (PubMed : 22719065).

See full target information NUP205

style

left-column

文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Discover oncology 15:338 PubMed39115575

2024

A metabolic reprogramming-related gene signature correlates with prognosis and proliferation of BLCA.

Applications

Unspecified application

Species

Unspecified reactive species

Yaoxin Wu,Yi Luo,Tinghao Li

Nature 613:575-581 PubMed36599981

2023

A quantitative map of nuclear pore assembly reveals two distinct mechanisms.

Applications

Unspecified application

Species

Unspecified reactive species

Shotaro Otsuka,Jeremy O B Tempkin,Wanlu Zhang,Antonio Z Politi,Arina Rybina,M Julius Hossain,Moritz Kueblbeck,Andrea Callegari,Birgit Koch,Natalia Rosalia Morero,Andrej Sali,Jan Ellenberg

eLife 7: PubMed30084827

2018

Nuclear pore heterogeneity influences HIV-1 infection and the antiviral activity of MX2.

Applications

Unspecified application

Species

Unspecified reactive species

Melissa Kane,Stephanie V Rebensburg,Matthew A Takata,Trinity M Zang,Masahiro Yamashita,Mamuka Kvaratskhelia,Paul D Bieniasz

Cell 173:958-971.e17 PubMed29628143

2018

Stress Granule Assembly Disrupts Nucleocytoplasmic Transport.

Applications

Unspecified application

Species

Unspecified reactive species

Ke Zhang,J Gavin Daigle,Kathleen M Cunningham,Alyssa N Coyne,Kai Ruan,Jonathan C Grima,Kelly E Bowen,Harsh Wadhwa,Peiguo Yang,Frank Rigo,J Paul Taylor,Aaron D Gitler,Jeffrey D Rothstein,Thomas E Lloyd

View all publications

style

left-column

style

left-column

style

right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NUP205抗体

Anti-NUP205 antibody

Immunoprecipitation - Anti-NUP205 antibody (AB157090)

Western blot - Anti-NUP205 antibody (AB157090)