Anti-Nup153抗体(ab84872)
Key features and details
- Rabbit polyclonal to Nup153
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-Nup153抗体
参阅全部 Nup153 一抗 -
描述
兔多克隆抗体to Nup153 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WBmore details -
种属反应性
与反应: Human
预测可用于: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
-
免疫原
Synthetic peptide corresponding to a region between residue 50 and 100 of human Nup153 (NP_005115.2).
-
常规说明
Concentration is optimized for IHC and not determined
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Purified using an epitope specific to NUP153 immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab84872于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | (3) |
1/100 - 1/500.
Formaldehyde fixed cells. Permeabilization with TritonX-100 is recommended. Acetone fixation is not recommended. |
| WB | (3) |
Use at an assay dependent concentration. Predicted molecular weight: 154 kDa. PubMed: 21858095
|
| 说明 |
|---|
|
ICC/IF
1/100 - 1/500. Formaldehyde fixed cells. Permeabilization with TritonX-100 is recommended. Acetone fixation is not recommended. |
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 154 kDa. PubMed: 21858095 |
靶标
-
功能
Possible DNA-binding subunit of the nuclear pore complex (NPC). The repeat-containing domain may be involved in anchoring components of the pore complex to the pore membrane. -
序列相似性
Contains 4 RanBP2-type zinc fingers. -
结构域
Contains F-X-F-G repeats. -
细胞定位
Nucleus > nuclear pore complex. Located to the terminal ring structure of the nucleoplasmic cage. - Information by UniProt
-
数据库链接
- Entrez Gene: 9972 Human
- Omim: 603948 Human
- SwissProt: P49790 Human
- Unigene: 601591 Human
- Unigene: 718703 Human
-
别名
- 153 kDa nucleoporin antibody
- HNUP153 antibody
- N153 antibody
see all
图片
-
Immunocytochemistry/ Immunofluorescence - Anti-Nup153 antibody (ab84872)Staining of human NUP153 in NBF fixed, asynchronous HeLa cells by Immunocytochemistry, using ab84872 at a dilution of 1/250. Detection: Red fluorescent goat anti-rabbit IgG used at a dilution of 1/100.
-
Immunocytochemistry/ Immunofluorescence - Anti-Nup153 antibody (ab84872)Staining of human NUP153 in NBF fixed asynchronous HeLa cells by Immunocytochemistry, using ab84872 at a dilution of 1/250. Detection: Red-fluorescent goat anti-rabbit IgG used at a dilution of 1/100.
数据表及文件
-
SDS download
-
Datasheet download
文献 (21)
ab84872 被引用在 21 文献中.
- Golchoubian B et al. Reticulon-like REEP4 at the inner nuclear membrane promotes nuclear pore complex formation. J Cell Biol 221:N/A (2022). PubMed: 34874453
- Damstra HGJ et al. Visualizing cellular and tissue ultrastructure using Ten-fold Robust Expansion Microscopy (TREx). Elife 11:N/A (2022). PubMed: 35179128
- Lv J et al. Gasdermin E mediates resistance of pancreatic adenocarcinoma to enzymatic digestion through a YBX1-mucin pathway. Nat Cell Biol 24:364-372 (2022). PubMed: 35292781
- Gleixner AM et al. NUP62 localizes to ALS/FTLD pathological assemblies and contributes to TDP-43 insolubility. Nat Commun 13:3380 (2022). PubMed: 35697676
- Guedán A et al. HIV-1 requires capsid remodelling at the nuclear pore for nuclear entry and integration. PLoS Pathog 17:e1009484 (2021). PubMed: 34543344